scholarly journals CO2 and HCO3- Permeability of the Rat Liver Mitochondrial Membrane

2016 ◽  
Vol 39 (5) ◽  
pp. 2014-2024 ◽  
Author(s):  
Mariela Arias-Hidalgo ◽  
Jan Hegermann ◽  
Georgios Tsiavaliaris ◽  
Fabrizio Carta ◽  
Claudiu T. Supuran ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mitochondrial Membrane ◽  
Gas Permeability ◽  
Alternative Pathway ◽  
Biological Membrane ◽  
Red Cell ◽  
Inner Mitochondrial Membrane ◽  
Red Cell Membrane ◽  
The Matrix ◽  
Rat Livers

Background/Aims: Across the mitochondrial membrane an exceptionally intense exchange of O2 and CO2 occurs. We have asked, 1) whether the CO2 permeability, PM,CO2, of this membrane is also exceptionally high, and 2) whether the mitochondrial membrane is sufficiently permeable to HCO3- to make passage of this ion an alternative pathway for exit of metabolically produced CO2. Methods: The two permeabilities were measured using the previously published mass spectrometric 18O exchange technique to study suspensions of mitochondria freshly isolated from rat livers. The mitochondria were functionally and morphologically in excellent condition. Results: The intramitochondrial CA activity was exclusively localized in the matrix. PM,CO2 of the inner mitochondrial membrane was 0.33 (SD ± 0.03) cm/s, which is the highest value reported for any biological membrane, even two times higher than PM,CO2 of the red cell membrane. PM,HCO3- was 2· 10-6 (SD ± 2· 10-6) cm/s and thus extremely low, almost 3 orders of magnitude lower than PM,HCO3- of the red cell membrane. Conclusion: The inner mitochondrial membrane is almost impermeable to HCO3- but extremely permeable to CO2. Since gas channels are absent, this membrane constitutes a unique example of a membrane of very high gas permeability due to its extremely low content of cholesterol.

CELL MEMBRANE CONSTITUENTS CONCERNED WITH TRANSPORT MECHANISMS

1964 ◽  
Vol 42 (6) ◽  
pp. 971-988 ◽  
Author(s):  
L. S. Wolfe
Keyword(s):  
Cell Membrane ◽  
Biological Membrane ◽  
Transport Processes ◽  
Neuronal Membrane ◽  
Red Cell ◽  
Transport Mechanisms ◽  
Red Cell Membrane ◽  
Membrane Atpase ◽  
Entire Cell ◽  
And Control

The biological membrane is a multiphasic, polyionic, regionally differentiated structure, the constituents of which are closely linked to the physiological and metabolic processes of the entire cell. Knowledge of the types of molecules, their orientation, and the relative importance of them for transport processes is still very fragmentary. The information at present available on the composition of the protoplast membrane, the red cell membrane, and the neuronal membrane is brought together and discussed in terms of the possible role in transport processes. A labile phosphate attached to protein or specific phosphatides or shared between them as a lipo-phosphoprotein complex is suggested as the intermediate in the active transport of sodium. The rapid phosphorylation of these constituents by ATP through the activity of membrane ATPase and their subsequent dephosphorylation could lead to rhythmic transitions in the configuration of membrane proteins and control active cation transport.

Ca2+ transport and permeability in inside-out red cell membrane vesicles after freezing

Cryobiology ◽  
1986 ◽  
Vol 23 (2) ◽  
pp. 134-140 ◽  
Author(s):  
A. Rubinacci ◽  
B. Fuller ◽  
F. Wuytack ◽  
W. De Loecker
Keyword(s):  
Cell Membrane ◽  
Membrane Vesicles ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Inside Out

scholarly journals Mechanical Properties of the Red Cell Membrane

1964 ◽  
Vol 4 (2) ◽  
pp. 115-135 ◽  
Author(s):  
R.P. Rand ◽  
A.C. Burton
Keyword(s):  
Mechanical Properties ◽  
Cell Membrane ◽  
Red Cell ◽  
Red Cell Membrane

scholarly journals Red Cell Membrane Permeability Deduced from Bulk Diffusion Coefficients

1974 ◽  
Vol 64 (6) ◽  
pp. 706-729 ◽  
Author(s):  
W. R. Redwood ◽  
E. Rall ◽  
W. Perl
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Diffusion Coefficients ◽  
Bulk Diffusion ◽  
Red Cells ◽  
Cell Membrane Permeability ◽  
Red Cell ◽  
Cell Column ◽  
Red Cell Membrane ◽  
One Dimensional

The permeability coefficients of dog red cell membrane to tritiated water and to a series of[14C]amides have been deduced from bulk diffusion measurements through a "tissue" composed of packed red cells. Red cells were packed by centrifugation inside polyethylene tubing. The red cell column was pulsed at one end with radiolabeled solute and diffusion was allowed to proceed for several hours. The distribution of radioactivity along the red cell column was measured by sequential slicing and counting, and the diffusion coefficient was determined by a simple plotting technique, assuming a one-dimensional diffusional model. In order to derive the red cell membrane permeability coefficient from the bulk diffusion coefficient, the red cells were assumed to be packed in a regular manner approximating closely spaced parallelopipeds. The local steady-state diffusional flux was idealized as a one-dimensional intracellular pathway in parallel with a one-dimensional extracellular pathway with solute exchange occurring within the series pathway and between the pathways. The diffusion coefficients in the intracellular and extracellular pathways were estimated from bulk diffusion measurements through concentrated hemoglobin solutions and plasma, respectively; while the volume of the extracellular pathway was determined using radiolabeled sucrose. The membrane permeability coefficients were in satisfactory agreement with the data of Sha'afi, R. I., C. M. Gary-Bobo, and A. K. Solomon (1971. J. Gen. Physiol. 58:238) obtained by a rapid-reaction technique. The method is simple and particularly well suited for rapidly permeating solutes.

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