scholarly journals Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples

Pathobiology ◽  
2016 ◽  
Vol 84 (2) ◽  
pp. 57-70 ◽  
Author(s):  
Hye-young Wang ◽  
Sungwoo Ahn ◽  
Sunyoung Park ◽  
SeungIl Kim ◽  
Hyeyoung Lee
Keyword(s):  
Breast Cancer ◽  
Quantitative Polymerase Chain Reaction ◽  
Growth Factor Receptor ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Epidermal Growth ◽  
Polymerase Chain ◽  
Formalin Fixed

scholarly journals Combined High Resistin and EGFR Expression Predicts a Poor Prognosis in Breast Cancer

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Yue Zeng ◽  
Chih-Hsin Tang ◽  
Yan Wang ◽  
Hua-Jun Lu ◽  
Bi-Fei Huang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Molecular Classification ◽  
Breast Cancer Tissue ◽  
Her2 Status ◽  
Cancer Tissue ◽  
Egfr Expression ◽  
Epidermal Growth

Elevated levels of resistin and epidermal growth factor receptor (EGFR) facilitate the development of breast cancer, although there are no reports of any correlation between these proteins. This study analyzed 392 human breast cancer tissue specimens and 42 samples of adjacent normal tissue. Rates of positive and strongly positive resistin expression were significantly higher in breast cancer tissue than in the adjacent nontumor tissue (83.2% vs. 23.8% and 20.9% vs. 0.0%, respectively; P < 0.001 for both comparisons). Positive resistin expression was significantly associated with tumor size, grade, stage, estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) status, and molecular classification; strongly positive resistin expression was associated with tumor grade, ER, PR, HER2 status, and molecular classification. Significantly positive correlations were observed between positive and strongly positive resistin expression and corresponding levels of EGFR expression. Relapse-free and overall survival was worse for patients with high levels of both proteins than for those with high levels of only one protein or normal levels of both proteins. Our evidence suggests that combined high levels of resistin and EGFR expression correlate with survival in patients with breast cancer.

scholarly journals Breast Cancer Clinical Trials: The Landscape at the Uganda Cancer Institute and Lessons Learned

2021 ◽  
pp. 127-132
Author(s):  
Manoj P. Menon ◽  
Nixon Niyonzima ◽  
Julie Gralow ◽  
Jackson Orem
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Clinical Trials ◽  
Estrogen Receptor ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Cancer Clinical Trials ◽  
Chain Reaction ◽  
Epidermal Growth ◽  
Polymerase Chain

The Uganda Cancer Institute, the sole national comprehensive cancer center in Uganda, has a long and rich history of clinical investigation and locally relevant cancer research. Given the increasing burden of breast cancer in Uganda and elsewhere in sub-Saharan Africa (SSA) and driven by the limited availability of immunohistochemistry (IHC), we launched a clinical trial aimed at evaluating locally available diagnostics to detect the presence of hormone receptors (estrogen receptor and progesterone receptor) and human epidermal growth factor receptor 2. Preliminary data from 32 women in the diagnostic component of the study reveal high sensitivity and specificity for estrogen receptor and progesterone receptor and high specificity for human epidermal growth factor receptor 2 when comparing reverse transcriptase polymerase chain reaction with the gold standard (IHC). Innovative diagnostic and treatment strategies are required to address the burden of breast cancer that is increasing throughout SSA. Given the costs, infrastructure, and trained personnel associated with IHC, alternative testing options (including reverse transcriptase polymerase chain reaction as tested in our study) may provide an expedited and cost-effective method to determine receptor testing in breast cancer. Clinical trials conducted in the local setting are critical to determining optimal strategies for effective breast cancer management in SSA.

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