Localization of α1-2 Fucose Glycan in the Mouse Olfactory Pathway

2016 ◽  
Vol 203 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Daisuke Kondoh ◽  
Akihiro Kamikawa ◽  
Motoki Sasaki ◽  
Nobuo Kitamura
Keyword(s):  
Olfactory Bulb ◽  
Olfactory System ◽  
Plexiform Layer ◽  
Basal Region ◽  
Main Olfactory Bulb ◽  
Olfactory Pathway ◽  
Ulex Europaeus ◽  
Receptor Cells ◽  
Ulex Europaeus Agglutinin I ◽  
Local Circuits

Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This study histochemically assessed the main olfactory and vomeronasal pathways in male and female ICR and C57BL/6J mice aged 3-4 months using UEA-I. Ulex europaeus agglutinin-I reacted with most receptor cells arranged mainly at the basal region of the olfactory epithelium. The olfactory nerve layer and glomerular layer of the main olfactory bulb were speckled with positive UEA-I staining, and positive fibers were scattered from the glomerular to the internal plexiform layer. The lateral olfactory tract and rostral migratory stream were also positive for UEA-I. We identified superficial short-axon cells, interneurons of the external plexiform layer, external, middle and internal tufted cells, mitral cells and granule cells as the origins of the UEA-I-positive fibers in the main olfactory bulb. The anterior olfactory nucleus, anterior piriform cortex and olfactory tubercle were negative for UEA-I. Most receptor cells in the vomeronasal epithelium and most glomeruli of the accessory olfactory bulb were positive for UEA-I. Our findings indicated that α1-2Fuc glycan is located within the primary and secondary, but not the ternary, pathways of the main olfactory system, in local circuits of the main olfactory bulb and within the primary, but not secondary, pathway of the vomeronasal system.

scholarly journals Paradoxically sparse chemosensory tuning in broadly-integrating external granule cells in the mouse accessory olfactory bulb

2019 ◽  
Author(s):  
Xingjian Zhang ◽  
Julian P. Meeks
Keyword(s):  
Patch Clamp ◽  
Olfactory Bulb ◽  
Olfactory System ◽  
Granule Cells ◽  
Ex Vivo ◽  
Accessory Olfactory Bulb ◽  
Main Olfactory Bulb ◽  
Patch Clamp Electrophysiology ◽  
Accessory Olfactory System ◽  
Critical Circuit

AbstractThe accessory olfactory bulb (AOB) is a critical circuit in the mouse accessory olfactory system (AOS), but AOB processing is poorly understood compared to the main olfactory bulb (MOB). We used 2-photon GCaMP6f Ca2+ imaging in an ex vivo preparation to study the chemosensory tuning of AOB external granule cells (EGCs), an interneuron population hypothesized to broadly integrate from mitral cells (MCs). We measured MC and EGC tuning to natural chemosignal blends and monomolecular ligands, finding that EGC tuning was far sparser than MC tuning. Simultaneous patch-clamp electrophysiology and Ca2+ imaging indicated that this was only partially explained by lower GCaMP6f-to-spiking ratios in EGCs compared to MCs. Ex vivo patch-clamp recordings revealed that EGC subthreshold responsivity was broad, but monomolecular ligand responses were insufficient to elicit spiking. These results indicate that EGC spiking is selectively engaged by chemosensory blends, suggesting different roles for EGCs than analogous interneurons in the MOB.

scholarly journals Postnatal Developmental Expression of Calbindin, Calretinin and Parvalbumin in Mouse Main Olfactory Bulb

2005 ◽  
Vol 37 (4) ◽  
pp. 276-282 ◽  
Author(s):  
Zhao-Ping Qin ◽  
Shu-Ming Ye ◽  
Ji-Zeng Du ◽  
Gong-Yu Shen
Keyword(s):  
Olfactory Bulb ◽  
Cell Layer ◽  
Plexiform Layer ◽  
Olfactory Nerve ◽  
Mitral Cell ◽  
Granule Cell Layer ◽  
Developmental Expression ◽  
Main Olfactory Bulb ◽  
Glomerular Layer ◽  
Layer 4

Abstract The distribution of calbindin, calretinin and parvalbumin during the development of the mouse main olfactory bulb (MOB) was studied using immunohistochemistry techniques. The results are as follows: (1) calbindin-immunoreactive profiles were mainly located in the glomerular layer, and few large calbindin-immunoreactive cells were found in the subependymal layer of postnatal day 10 (P1 0) to postnatal day 40 (P40) mice; (2) no calbindin was detected in the mitral cell layer at any stage; (3) calretinin-immunoreactive profiles were present in all layers of the main olfactory bulb at all stages, especially in the olfactory nerve layer, glomerular layer and granule cell layer; (4) parvalbumin-immunoreactive profiles were mainly located in the external plexiform layer (except for P10 mice); (5) weakly stained parvalbumin-immunoreactive profiles were present in the glomerular layer at all stages; and (6) no parvalbumin was detected in the mitral cell layer at any stage.

scholarly journals Somatostatin interneurons delineate the inner part of the external plexiform layer in the mouse main olfactory bulb

2010 ◽  
Vol 518 (11) ◽  
pp. 1976-1994 ◽  
Author(s):  
Gabriel Lepousez ◽  
Zsolt Csaba ◽  
Véronique Bernard ◽  
Catherine Loudes ◽  
Catherine Videau ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Plexiform Layer ◽  
Main Olfactory Bulb

scholarly journals Changes in weak pair-wise correlations during running reshapes network state in the main olfactory bulb

2020 ◽  
Author(s):  
Udaysankar Chockanathan ◽  
Emily J. W. Crosier ◽  
Spencer Waddle ◽  
Edward Lyman ◽  
Richard C. Gerkin ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Spontaneous Activity ◽  
Olfactory System ◽  
Activity Patterns ◽  
Higher Order ◽  
High Density ◽  
Main Olfactory Bulb ◽  
Population Activity ◽  
Maximum Entropy Models ◽  
Electrophysiological Recordings

AbstractNeural codes for sensory representations are thought to reside in a broader space defined by the patterns of spontaneous activity that occur when stimuli are not being presented. To understand the structure of this spontaneous activity in the olfactory system, we performed high-density recordings of population activity in the main olfactory bulb of awake mice. We found that spontaneous activity patterns of ensembles of mitral and tufted (M/T) cells in the main olfactory bulb changed dramatically during locomotion, including decreases in pairwise correlations between neurons and increases in the entropy of the population. Maximum entropy models of the ensemble activity revealed that pair-wise interactions were better at predicting patterns of activity when the animal was stationary than while running, suggesting that higher order (3rd, 4th order) interactions between neurons shape activity during locomotion. Taken together, we found that locomotion influenced the structure of spontaneous population activity at the earliest stages of olfactory processing, 1 synapse away from the sensory receptors in the nasal epithelium.New and NoteworthyThe organization and structure of spontaneous population activity in the olfactory system places constraints of how odor information is represented. Using high-density electrophysiological recordings of mitral and tufted cells, we found that running increases the dimensionality of spontaneous activity, implicating higher-order interactions among neurons during locomotion. Behavior thus flexibly alters neuronal activity at the earliest stages of sensory processing.

scholarly journals Sensory Deafferentation Transsynaptically Alters Neuronal GluR1 Expression in the External Plexiform Layer of the Adult Mouse Main Olfactory Bulb

2008 ◽  
Vol 33 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Kathryn A. Hamilton ◽  
Stephanie Parrish-Aungst ◽  
Frank L. Margolis ◽  
Ferenc Erdélyi ◽  
Gabor Szabó ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Adult Mouse ◽  
Plexiform Layer ◽  
Main Olfactory Bulb

scholarly journals 50 years of decoding olfaction

2018 ◽  
Vol 2 ◽  
pp. 239821281881749 ◽  
Author(s):  
Peter A Brennan
Keyword(s):  
Olfactory Bulb ◽  
Olfactory System ◽  
Piriform Cortex ◽  
Vomeronasal System ◽  
Main Olfactory Bulb ◽  
Late 20Th Century ◽  
Fundamental Insight ◽  
Main Olfactory System ◽  
G Protein Coupled

The identification, in the late 20th century, of unexpectedly large families of G-protein-coupled chemosensory receptors revolutionised our understanding of the olfactory system. The discovery that non-selective olfactory sensory neurons express a single olfactory receptor type and project to a specific glomerulus in the main olfactory bulb provided fundamental insight into the spatial pattern of odour representation in the main olfactory bulb. Studies using head-fixed awake mice and optogenetics have revealed the importance of the timing of glomerular input in relation to the sniff cycle and the role of piriform cortex in odour object recognition. What in the 1970s had appeared to be a relatively simple dichotomy between odour detection by the main olfactory system and pheromone detection by the vomeronasal system has been found to consist of multiple subsystems. These mediate innate responses to odours and pheromones and to substances as diverse as O2, volatile urinary constituents, peptides and proteins.

scholarly journals Changes in pairwise correlations during running reshape global network state in the main olfactory bulb

2021 ◽  
Vol 125 (5) ◽  
pp. 1612-1623
Author(s):  
Udaysankar Chockanathan ◽  
Emily J. W. Crosier ◽  
Spencer Waddle ◽  
Edward Lyman ◽  
Richard C. Gerkin ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Sensory Processing ◽  
Olfactory System ◽  
Global Network ◽  
Main Olfactory Bulb ◽  
Population Activity ◽  
Network State ◽  
Electrophysiological Recordings ◽  
Pairwise Correlations ◽  
Locomotion Behavior

The organization and structure of spontaneous population activity in the olfactory system places constraints of how odor information is represented. Using high-density electrophysiological recordings of mitral and tufted cells, we found that running increases the dimensionality of spontaneous activity, implicating higher order interactions among neurons during locomotion. Behavior, thus, flexibly alters neuronal activity at the earliest stages of sensory processing.

The embryonic development of the olfactory system in Amphiprion melanopus (Perciformes: Pomacentridae) related to the host imprinting hypothesis

2000 ◽  
Vol 80 (6) ◽  
pp. 1103-1109 ◽  
Author(s):  
Michael Arvedlund ◽  
Kim Larsen ◽  
Heather Winsor
Keyword(s):  
Embryonic Development ◽  
Olfactory Bulb ◽  
Olfactory System ◽  
Olfactory Placode ◽  
Receptor Cells ◽  
Amphiprion Melanopus

Development of the olfactory system in anemonefish embryos of the species Amphiprion melanopus was examined from day six post-fertilization, until hatching (day nine). An olfactory placode with receptor cells lining the epithelium and nerve axons from the placode into the olfactory bulb, was observed on newly hatched embryos. In addition, two different secondary bilateral receptor systems were found. These findings may firstly support the anemonefish host imprinting hypothesis, and secondly indicate that the ontogenetic timing of this imprinting mechanism occurs towards the end of the embryonic development.

Sign in / Sign up

Export Citation Format

Share Document