Selective COX-2 Inhibition Exerts No Negative Effects on Peripheral Blood Lymphocytes in Allergic Asthmatics

2016 ◽  
Vol 170 (1) ◽  
pp. 57-61
Author(s):  
Guro Gafvelin ◽  
Jeanette Grundström ◽  
Maria Edin Grimheden ◽  
Sara Sánchez Vidaurre ◽  
Kameran Daham ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Negative Effects ◽  
Blood Lymphocytes ◽  
Cox 2

scholarly journals Effects of Glucomannan on the Sacculus Rotundus and Peripheral Blood Lymphocytes in New Zealand Rabbits during Aflatoxicosis

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Emrah Sur ◽  
Hasan Hüseyin Dönmez ◽  
Murat Boydak ◽  
Mehmet Bozkurt Ataman
Keyword(s):  
New Zealand ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Negative Effects ◽  
Blood Lymphocytes ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Sacculus Rotundus ◽  
Naphthyl Acetate ◽  
New Zealand Rabbits

This study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical methods. Twenty-four adult rabbits of both sexes were divided into four equal groups, namely, as control, glucomannan 0.2 g/day, AF 125 μg/kg/day, and glucomannan combined with AF. The animals in all groups were treated for 12 weeks by the above-mentioned diet. When compared to control, AF-treatment caused significant decrease in alpha-naphthyl acetate esterase- (ANAE-) positive peripheral blood lymphocyte (PBL) percentages. The addition of the glucomannan to AFcontaining diet recovered the adverse effects of AF on sacculus rotundus and increased the ANAE-positive PBL counts. These results suggested that glucomannan was effective against the negative effects of AF in rabbits.

Semiautomated microfluorometric instrument and reagent system for monitoring disease-related immunosuppression.

1982 ◽  
Vol 28 (9) ◽  
pp. 1887-1893 ◽  
Author(s):  
D F Ranney ◽  
A J Quattrone
Keyword(s):  
Immune Response ◽  
Dna Content ◽  
Peripheral Blood ◽  
Fluorescence Enhancement ◽  
Photon Counting ◽  
Peripheral Blood Lymphocytes ◽  
Response Modulation ◽  
Blood Lymphocytes ◽  
Complex Test ◽  
Immune Response Modulation

Abstract Several common metabolites and drugs in the serum in of patients with inflammatory, infectious, autoimmune, immunodeficient, neoplastic, and toxicant-induced diseases can produce artifactual suppression of the [methyl-3H]-thymidine assay, which is widely used to evaluate lymphocyte responsiveness. We have developed a sensitive, semiautomated, fluorescence-enhancement assay in which true immunosuppressors are measured in the presence of absence of such interfering substances. Peripheral blood lymphocytes are activated with mitogens in standard microtiter culture trays. Changes in lymphocyte DNA content are quantified with a reagent formulation containing mithramycin, the fluorescence of which is enhanced on binding to DNA in the presence of MgCl2. We solubilize cells within the intact microtiter tray by using an automated, inverted "Array Sonicator," and measure fluorescence with an automated, photon-counting fluorometer. With this system, immune response modulation can be accurately assessed in the presence of patients' sera and other complex test substances (e.g., supernates from hybridomas, fermentation vats, viral preparations, and macrophage cultures.

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