scholarly journals Gene Therapy for Pyoderma Gangrenosum: Optimal Transfection Conditions and Effect of Drugs on Gene Delivery in the HaCaT Cell Line Using Cationic Liposomes

2016 ◽  
Vol 29 (3) ◽  
pp. 119-129 ◽  
Author(s):  
Alexandra R. Teagle ◽  
James C. Birchall ◽  
Rachel Hargest
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Cell Line ◽  
Pyoderma Gangrenosum ◽  
Hacat Cell ◽  
Cationic Liposomes ◽  
Hacat Cell Line

scholarly journals Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.

1988 ◽  
Vol 106 (3) ◽  
pp. 761-771 ◽  
Author(s):  
P Boukamp ◽  
R T Petrussevska ◽  
D Breitkreutz ◽  
J Hornung ◽  
A Markham ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Human Skin ◽  
Cell Lines ◽  
Hacat Cell ◽  
Epithelial Cell Line ◽  
Spontaneous Transformation ◽  
Chromosomal Alterations ◽  
Hacat Cell Line

In contrast to mouse epidermal cells, human skin keratinocytes are rather resistant to transformation in vitro. Immortalization has been achieved by SV40 but has resulted in cell lines with altered differentiation. We have established a spontaneously transformed human epithelial cell line from adult skin, which maintains full epidermal differentiation capacity. This HaCaT cell line is obviously immortal (greater than 140 passages), has a transformed phenotype in vitro (clonogenic on plastic and in agar) but remains nontumorigenic. Despite the altered and unlimited growth potential, HaCaT cells, similar to normal keratinocytes, reform an orderly structured and differentiated epidermal tissue when transplanted onto nude mice. Differentiation-specific keratins (Nos. 1 and 10) and other markers (involucrin and filaggrin) are expressed and regularly located. Thus, HaCaT is the first permanent epithelial cell line from adult human skin that exhibits normal differentiation and provides a promising tool for studying regulation of keratinization in human cells. On karyotyping this line is aneuploid (initially hypodiploid) with unique stable marker chromosomes indicating monoclonal origin. The identity of the HaCaT line with the tissue of origin was proven by DNA fingerprinting using hypervariable minisatellite probes. This is the first demonstration that the DNA fingerprint pattern is unaffected by long-term cultivation, transformation, and multiple chromosomal alterations, thereby offering a unique possibility for unequivocal identification of human cell lines. The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.

scholarly journals Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos

2019 ◽  
Vol 16 ◽  
pp. 2108-2114
Author(s):  
Wastuti Hidayati Suriyah ◽  
Abdul Razak Kasmuri ◽  
Fiona How Ni Foong ◽  
Dhona Afriza ◽  
Solachuddin Jauhari Arief Ichwan
Keyword(s):  
Oral Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Hacat Cell ◽  
Toxic Effects ◽  
Zebrafish Embryos ◽  
Hacat Cell Line

Study of Anti-atopic Dermatitis Effects of Juice of Raphanus sativus var in HaCaT Cell Line

KSBB Journal ◽  
2017 ◽  
Vol 32 (4) ◽  
pp. 311-318 ◽  
Author(s):  
Seung-Je Lee ◽  
Eun-Gyeong Lim ◽  
Ga-Yeon Kim ◽  
Mi-Ji Jeon ◽  
Sang-Yong Kim ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Cell Line ◽  
Raphanus Sativus ◽  
Hacat Cell ◽  
Hacat Cell Line

Photoprotective efficiency of PLGA-curcumin nanoparticles versus curcumin through the involvement of ERK/AKT pathway under ambient UV-R exposure in HaCaT cell line

Biomaterials ◽  
2016 ◽  
Vol 84 ◽  
pp. 25-41 ◽  
Author(s):  
Deepti Chopra ◽  
Lipika Ray ◽  
Ashish Dwivedi ◽  
Shashi Kant Tiwari ◽  
Jyoti Singh ◽  
...  
Keyword(s):  
Cell Line ◽  
Hacat Cell ◽  
Akt Pathway ◽  
Curcumin Nanoparticles ◽  
Hacat Cell Line

Potential Antipsoriatic Avarol Derivatives as Antioxidants and Inhibitors of PGE2Generation and Proliferation in the HaCaT Cell Line

2004 ◽  
Vol 67 (9) ◽  
pp. 1459-1463 ◽  
Author(s):  
Maria Amigó ◽  
Maria Carmen Terencio ◽  
Maya Mitova ◽  
Carmine Iodice ◽  
Miguel Payá ◽  
...  
Keyword(s):  
Cell Line ◽  
Hacat Cell ◽  
Hacat Cell Line

scholarly journals The Cytotoxic and Anti-proliferative Activity of High Molecular Weight Pectin and Modified Citrus Pectin

2016 ◽  
Vol 6 (9) ◽  
pp. 587 ◽  
Author(s):  
Roula  Abdel-Massih ◽  
Venicia Hawach ◽  
Marie-Anne Boujaoude
Keyword(s):  
Molecular Weight ◽  
Cell Line ◽  
High Molecular Weight ◽  
Proliferative Activity ◽  
Hacat Cell ◽  
Dependent Manner ◽  
Citrus Pectin ◽  
Hacat Cell Line ◽  
Proliferative Effect ◽  
Most Probable Explanation

Background: Pectin is a heterogeneous polysaccharide mainly present in citrus fruits and has different biological activities.Objective: High molecular weight Citrus Pectin and modified citrus pectin (MCP) were tested for their cytotoxic, anti-proliferative, and anti-oxidant activity.Methods: The cytotoxicity of pectin was studied against HaCaT cell line (human keratinocyte cell line) using Trypan blue method and LDH-cytotoxicity assay. Anti-proliferative activity was assayed using a WST-1 proliferation kit. Antioxidant activity was determined using the DPPH scavenging assay.Results: MCP and Pectin both reduced the viability of HaCaT cells in a dose dependent manner; however MCP was found to be more cytotoxic than high molecular weight citrus pectin since it had a lower IC50 (300ug/ul). At non-cytotoxic concentrations, the viability of cells decreased with increase of concentration of MCP as determined by the WST-1. MCP exhibited a higher antioxidant effect than pectin (SC50 at a concentration range between 2 and 4mg/ml).Conclusion: This study suggests that MCP exhibits a stronger cytotoxic and anti-proliferative effect on HaCaT cell line than pectin. The most probable explanation of this observation is the different effect due to the variable molecular weight and exposed side-chains of MCP and high molecular weight citrus pectin. Keywords: Cytotoxic, Anti-proliferative, Pectin, MCP, HaCaT cell line 

Cytotoxicity of protic ionic liquids towards the HaCat cell line derived from human skin

2020 ◽  
Vol 314 ◽  
pp. 113602 ◽  
Author(s):  
Radhika Arunkumar ◽  
Amanda N. Abraham ◽  
Ravi Shukla ◽  
Calum J. Drummond ◽  
Tamar L. Greaves
Keyword(s):  
Ionic Liquids ◽  
Cell Line ◽  
Human Skin ◽  
Hacat Cell ◽  
Protic Ionic Liquids ◽  
Hacat Cell Line

scholarly journals (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line

2020 ◽  
Vol 21 (4) ◽  
pp. 1449 ◽  
Author(s):  
Eliana B. Souto ◽  
Aleksandra Zielinska ◽  
Selma B. Souto ◽  
Alessandra Durazzo ◽  
Massimo Lucarini ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cell Line ◽  
Hacat Cell ◽  
Aqueous Dispersion ◽  
Glycerol Monostearate ◽  
Cell Viability Assay ◽  
Hacat Cell Line ◽  
Viability Assay ◽  
Zero Order Kinetics ◽  
Keratinocyte Cell

In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed.

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