scholarly journals 5-Methylcytosine-Rich Heterochromatin in the Indian Muntjac

2015 ◽  
Vol 147 (4) ◽  
pp. 240-246 ◽  
Author(s):  
Michael Schmid ◽  
Claus Steinlein ◽  
Christian Lomb ◽  
Karl Sperling ◽  
Heidemarie Neitzel
Keyword(s):  
X Chromosome ◽  
Centromeric Region ◽  
Uv Light ◽  
Chromosomal Dna ◽  
Metaphase Chromosomes ◽  
Indian Muntjac ◽  
Uv Light Irradiation ◽  
Major Region ◽  
Inactivation Process ◽  
A Minor

Two 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the ‘neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in the centromeric region of the free autosome 3 after denaturing the chromosomal DNA for 3 h or longer. The structure and possible function of the major hypermethylated region as barrier against spreading of the X-inactivation process into the autosome 3 is discussed.

scholarly journals Purification of the chromosomes of the Indian muntjac by flow sorting.

1976 ◽  
Vol 24 (1) ◽  
pp. 348-354 ◽  
Author(s):  
A V Carrano ◽  
J W Gray ◽  
D H Moore ◽  
J L Minkler ◽  
B H Mayall ◽  
...  
Keyword(s):  
Large Fraction ◽  
Centromere Region ◽  
Chromosomal Dna ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Indian Muntjac ◽  
Flow Sorting ◽  
Flow Microfluorometry ◽  
Mechanical Shearing ◽  
High Degree

Metaphase chromosomes were isolated from a male Indian muntjac cell line, were stained with ethidium bromide and were analyzed by flow microfluorometry to establish a deoxyribonucleic acid (DNA)-based karyotype. Five major peaks were evident on the chromosomal DNA distribution corresponding to the five chromosome types in this species. The amount of DNA in each chromosome was confirmed by cytophotometric measurements of intact metaphase spreads. The five chromosome types were separated by flow sorting at rates up to several hundred chromosomes per second. The sorted chromosomes were identified by morphology and by Giemsa banding patterns. The automsomes, Numbers 1, 2 and 3, and the X + 3 composite chromosome were separated with a high degree of purity (90%). The centromere region of the X + 3 chromosome was fragile to mechanical shearing, and during isolation a small proportion of these chromosomes broke into four segiments: the long arm, the short arm, the short arm plus centromere and the centromere region. A large fraction of the constitutive heterochromatin of this species is present in the centromere region of the X + 3 chromosome and in the Y chromosome; these two regions possess similar amounts of DNA and therefore sort together. Chromosome flow sorting is rapid, reproducible and precise; it allows the collection of microgram quantities of purified chromosomes.

Synthesis, crystal structure, luminescence, and photocatalytic properties of a 2D Cu(II) metal-organic frameworks based on 3,5-di(1H-benzimidazol-1-yl)pyridine and 4,4′-oxybis(benzoate) ligands

2020 ◽  
Vol 75 (8) ◽  
pp. 727-732
Author(s):  
Chen Zhang ◽  
Jian-Qing Tao
Keyword(s):  
Crystal Structure ◽  
Thermal Analysis ◽  
Uv Light ◽  
Metal Organic Framework ◽  
Metal Organic Frameworks ◽  
X Ray Diffraction ◽  
X Ray ◽  
Uv Light Irradiation ◽  
Photocatalytic Activities ◽  
Metal Organic

AbstractA new Cu(II) metal-organic framework, [Cu(L)(OBA)·H2O]n (1) [H2OBA = 4,4′-oxybis(benzoic acid), L = 3,5-di(1H-benzimidazol-1-yl)pyridine] was hydrothermally synthesized and characterized through IR spectroscopy, elemental and thermal analysis and single-crystal X-ray diffraction. Complex 1 is a four-connected uni-nodal 2D net with a (44·62) topology which shows an emission centered at λ ∼393 nm upon excitation at λ = 245 nm. Moreover, complex 1 possesses high photocatalytic activities for the decomposition of Rhodamine B (RhB) under UV light irradiation.

scholarly journals TiO2-Powdered Activated Carbon (TiO2/PAC) for Removal and Photocatalytic Properties of 2-Methylisoborneol (2-MIB) in Water

Water ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1622
Author(s):  
Xiao-Pin Guo ◽  
Peng Zang ◽  
Yong-Mei Li ◽  
Dong-Su Bi
Keyword(s):  
Activated Carbon ◽  
Natural Organic Matter ◽  
Uv Light ◽  
Sol Gel ◽  
Light Irradiation ◽  
Nano Tio2 ◽  
Uv Light Irradiation ◽  
Adsorption Capacities ◽  
Taste And Odor ◽  
Composite Photocatalysts

2-methylisoborneol (2-MIB) is a common taste and odor compound caused by off-flavor secondary metabolites, which represents one of the greatest challenges for drinking water utilities worldwide. A TiO2-coated activated carbon (TiO2/PAC) has been synthesized using the sol-gel method. A new TiO2/PAC photocatalyst has been successfully employed in photodegradation of 2-MIB under UV light irradiation. In addition, the combined results of XRD, SEM-EDX, FTIR and UV-Vis suggested that the nano-TiO2 had been successfully loaded on the surface of PAC. Experimental results of 2-MIB removal indicated that the adsorption capacities of PAC for 2-MIB were higher than that of TiO2/PAC. However, in the natural organic matter (NOM) bearing water, the removal efficiency of 2-MIB by TiO2/PAC and PAC were 97.8% and 65.4%, respectively, under UV light irradiation. Moreover, it was shown that the presence of NOMs had a distinct effect on the removal of MIB by TiO2/PAC and PAC. In addition, a simplified equivalent background compound (SEBC) model could not only be used to describe the competitive adsorption of MIB and NOM, but also represent the photocatalytic process. In comparison to other related studies, there are a few novel composite photocatalysts that could efficiently and rapidly remove MIB by the combination of adsorption and photocatalysis.

Photocatalytic performance of Cu-doped titania thin films under UV light irradiation

2021 ◽  
Vol 553 ◽  
pp. 149535
Author(s):  
Elisa Moretti ◽  
Elti Cattaruzza ◽  
Cristina Flora ◽  
Aldo Talon ◽  
Eugenio Casini ◽  
...  
Keyword(s):  
Thin Films ◽  
Photocatalytic Performance ◽  
Uv Light ◽  
Light Irradiation ◽  
Uv Light Irradiation ◽  
Doped Titania

Amino acid-templated zinc phosphites: low-dimensional structures, fluorescence, and nonlinear optical properties

2021 ◽  
Author(s):  
Yumei Mao ◽  
Xuehua Dong ◽  
Yuandan Deng ◽  
Jing Li ◽  
Ling Huang ◽  
...  
Keyword(s):  
Optical Properties ◽  
Amino Acid ◽  
Nonlinear Optical ◽  
Uv Light ◽  
Nonlinear Optical Properties ◽  
Light Irradiation ◽  
Blue Fluorescence ◽  
Structure Directing Agent ◽  
Uv Light Irradiation ◽  
Low Dimensional

Two new zinc phosphites were prepared using the amino acid alanine as structure-directing agent. They have tubular and ladder-like structures exhibiting blue fluorescence upon UV light irradiation. Notably, the tubular...

Photodegradation of Polychlorinated Dibenzo-p-dioxins in Liquid Samples by Near UV Light Irradiation

1996 ◽  
Vol 54 (4) ◽  
pp. 331-337 ◽  
Author(s):  
M.Perla Colombini ◽  
Fabio Di Francesco ◽  
Roger Fuoco
Keyword(s):  
Uv Light ◽  
Light Irradiation ◽  
Liquid Samples ◽  
Uv Light Irradiation

scholarly journals Synthesis of Amorphous CS2 by UV Light Irradiation

1997 ◽  
Vol 105 (1219) ◽  
pp. 272-274
Author(s):  
Yutaka TAKAHASHI ◽  
Shigeo KOTAKE ◽  
Toshihiko OHTA ◽  
Akihito MATSUMURO ◽  
Masafumi SENOO
Keyword(s):  
Uv Light ◽  
Light Irradiation ◽  
Uv Light Irradiation
Sign in / Sign up

Export Citation Format

Share Document