scholarly journals Concurrent Gastric Adenocarcinoma of Fundic Gland Type and Carcinoma with Lymphoid Stroma: A Rare Case Report

2016 ◽  
Vol 10 (2) ◽  
pp. 292-301 ◽  
Author(s):  
Hee Jeong Cha ◽  
Kyungbin Kim ◽  
Misung Kim ◽  
Hyejeong Choi ◽  
Young Min Kim ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Gastric Adenocarcinoma ◽  
Body Mass ◽  
Upper Body ◽  
Fundic Gland ◽  
Lower Body ◽  
Barr Virus ◽  
Lymphoid Stroma ◽  
Gland Type

Both gastric adenocarcinoma of fundic gland type (ADC-FG) and carcinoma with lymphoid stroma (lymphoepithelioma-like carcinoma, LELC) are relatively rare. Epstein-Barr virus (EBV) has been implicated in the pathogenesis of LELC. However, the pathogenesis of ADC-FG, as well as the role of EBV in the carcinogenesis of LELC, remain unclear and under debate. The current study presents a case of concurrent ADC-FG and LELC in the stomach in a 69-year-old man. Total gastrectomy was performed, and two separate masses were identified. Upon histological and immunohistochemical examination, the mass located in the lower body was determined to be LELC and the mass in the upper body was diagnosed as ADC-FG. The lesions were characterized by different mucin phenotypes and EBV in situ results. In the lower-body mass, EBV in situ hybridization expression was diffusely strongly positive, but MUC2, MUC5AC, MUC6, and CD10 were all negative. On the other hand, in the upper-body mass, the results were positive for MUC6 but negative for MUC2, MUC5AC, CD10, and EBV by in situ hybridization. The remaining gastric tissue was unremarkable, and perigastric lymph node metastases were absent. Seven months after the gastrectomy, a postoperative computed tomography scan revealed no recurrence or metastasis.

scholarly journals LGR5 expression is associated with prognosis in poorly differentiated gastric adenocarcinoma

2020 ◽  
Author(s):  
Takehito Ehara ◽  
Takeshi Uehara ◽  
Tomoyuki Nakajima ◽  
Yasuhiro Kinugawa ◽  
Shota Kobayashi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Gastric Adenocarcinoma ◽  
Stem Cell Marker ◽  
Rank Test ◽  
High Group ◽  
Barr Virus ◽  
Lgr5 Expression ◽  
Poorly Differentiated ◽  
Epstein Barr

Abstract Background Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is an important cancer stem cell marker in gastric cancer. However, no detailed studies are available on LGR5 expression in poorly differentiated gastric adenocarcinoma (PD-AC). Therefore, we investigated the relationship between LGR5 expression and clinicopathological data in PD-AC. Methods LGR5 expression was identified in 41 PD-AC cases using RNAscope, which is a highly sensitive RNA in situ hybridization method. Epstein–Barr virus (EBV) infection was also detected by EBV in situ hybridization. Results LGR5 expression was identified in 38 of 41 PD-AC cases, and 17 cases were identified as LGR5 high. The frequency of EBV positivity tended to be higher in the LGR5 -low group than in the LGR5 -high group (P=0.0764). Furthermore, the frequency of vascular invasion tended to be higher in the LGR5 -high group than in the LGR5 -low group (P=0.0764). The overall survival of PD-AC patients in the LGR5 -high group was significantly lower than in the LGR5 -low group (log-rank test, P=0.0108). The Cox proportional hazard regression model revealed that the LGR5 -low group (HR=0.29; 95% CI: 0.11–0.74; P=0.01) showed independently better OS for PD-AC. Conclusions The correlation between high LGR5 expression and poor prognosis in PD-AC may be applicable to the development of LGR5 targeted therapy and prognostic markers. Further study is warranted.

Computed tomography radiomics to predict EBER positivity in Epstein–Barr virus-associated gastric adenocarcinomas: a retrospective study

2021 ◽  
pp. 028418512110290
Author(s):  
Cheng Zhang ◽  
Hai-lin Wen ◽  
Rong Zhang ◽  
Shu-yi Xie ◽  
Chuan-miao Xie
Keyword(s):  
Computed Tomography ◽  
In Situ Hybridization ◽  
Decision Tree ◽  
Gastric Adenocarcinoma ◽  
Epstein Barr Virus ◽  
Decision Tree Model ◽  
Tree Model ◽  
Barr Virus ◽  
Epstein Barr

Background The relevance of Epstein–Barr virus (EBV) in gastric carcinoma has been represented by the existence of EBV-encoded small RNA (EBER) in the tumor cells and has prognostic significance in gastric cancer, while gastric adenocarcinoma represents the most frequently occurring gastric malignancy. Purpose To observe the capacity of radiomic features extracted from contrast-enhanced computed tomography (CE-CT) images to differentiate EBER-positive gastric adenocarcinoma from EBER-negative ones. Material and Methods A total of 54 patients with gastric adenocarcinoma (EBER-positive: 27, EBER-negative: 27) were retrospectively examined. Radiomic imaging features were extracted from all regions of interest (ROI) delineated by two experienced radiologists on late arterial phase CT images. We distinguished related radiomic features through the two-tailed t test and applied them to construct a decision tree model to evaluate whether EBER in situ hybridization positive had appeared. Results Nine radiomics features were significantly related to EBER in situ hybridization status ( P < 0.05), four of which were used to build the decision tree through backward elimination: Correlation_ AllDirection_offset7, Correlation_ angle135_offset7, RunLengthNonuniformity_ AllDirection_offset1_SD, and HighGreyLevelRunEmphasis_ AllDiretion_offset1_SD. The decision tree model consisted of seven decision nodes and six terminal nodes, three of which demonstrated positive EBER in situ hybridization. The specificity, sensitivity, and accuracy of the model were 84%, 80%, and 81.7%, respectively. The area under the curve of the decision tree model was 0.87. Conclusion Radiomics based on CE-CT could be applied to predict EBER in situ hybridization status preoperatively in patients with gastric adenocarcinoma.

scholarly journals Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection

2012 ◽  
Vol 43 (1) ◽  
pp. 393-404 ◽  
Author(s):  
Marcos Antonio Pereira de Lima ◽  
Márcia Valéria Pitombeira Ferreira ◽  
Marcos Aurélio Pessoa Barros ◽  
Maria Inês de Moura Campos Pardini ◽  
Adriana Camargo Ferrasi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr

The impact of Epstein-Barr virus status on clinical outcome in diffuse large B-cell lymphoma

Blood ◽  
2007 ◽  
Vol 110 (3) ◽  
pp. 972-978 ◽  
Author(s):  
Sarah Park ◽  
Jeeyun Lee ◽  
Young Hyeh Ko ◽  
Arum Han ◽  
Hyun Jung Jun ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
B Cell ◽  
Epstein Barr Virus ◽  
Progression Free Survival ◽  
B Cell Lymphoma ◽  
Free Survival ◽  
Barr Virus ◽  
Large B Cell Lymphoma ◽  
Epstein Barr

AbstractTo define prognostic impact of Epstein-Barr virus (EBV) infection in diffuse large B-cell lymphoma (DLBCL), we investigated EBV status in patients with DLBCL. In all, 380 slides from paraffin-embedded tissue were available for analysis by EBV-encoded RNA-1 (EBER) in situ hybridization, and 34 cases (9.0%) were identified as EBER-positive. EBER positivity was significantly associated with age greater than 60 years (P = .005), more advanced stage (P < .001), more than one extranodal involvement (P = .009), higher International Prognostic Index (IPI) risk group (P = .015), presence of B symptom (P = .004), and poorer outcome to initial treatment (P = .006). The EBER+ patients with DLBCL demonstrated substantially poorer overall survival (EBER+ vs EBER− 35.8 months [95% confidence interval (CI), 0-114.1 months] vs not reached, P = .026) and progression-free survival (EBER+ vs EBER− 12.8 months [95% CI, 0-31.8 months] vs 35.8 months [95% CI, 0-114.1 months], respectively (P = .018). In nongerminal center B-cell–like subtype, EBER in situ hybridization positivity retained its statistical significance at the multivariate level (P = .045). Nongerminal center B-cell–like patients with DLBCL with EBER positivity showed substantially poorer overall survival with 2.9-fold (95% CI, 1.1-8.1) risk for death. Taken together, DLBCL patients with EBER in situ hybridization+ pursued more rapidly deteriorating clinical course with poorer treatment response, survival, and progression-free survival.

scholarly journals Gastric adenocarcinoma of fundic gland type spreading to heterotopic gastric glands

2017 ◽  
Vol 23 (38) ◽  
pp. 7047-7053 ◽  
Author(s):  
Shigeo Manabe ◽  
Ken-ichi Mukaisho ◽  
Takayuki Yasuoka ◽  
Fumitaka Usui ◽  
Tatsuzo Matsuyama ◽  
...  
Keyword(s):  
Gastric Adenocarcinoma ◽  
Fundic Gland ◽  
Gastric Glands ◽  
Gland Type
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