scholarly journals Extracellular Vesicles from Red Blood Cell Products Induce a Strong Pro-Inflammatory Host Response, Dependent on Both Numbers and Storage Duration

2015 ◽  
Vol 43 (4) ◽  
pp. 302-305 ◽  
Author(s):  
Marleen Straat ◽  
Anita N. Böing ◽  
Anita Tuip-De Boer ◽  
Rienk Nieuwland ◽  
Nicole P. Juffermans
Keyword(s):  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Host Response ◽  
Storage Duration ◽  
And Storage

scholarly journals Processing methods and storage duration impact extracellular vesicle counts in red blood cell units

2020 ◽  
Vol 4 (21) ◽  
pp. 5527-5539
Author(s):  
Clementine Gamonet ◽  
Maxime Desmarets ◽  
Guillaume Mourey ◽  
Sabeha Biichle ◽  
Sophie Aupet ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Annexin V ◽  
Analysis Of Covariance ◽  
Active Components ◽  
Storage Duration ◽  
Processing Methods ◽  
Controllable Factors ◽  
Filter Type ◽  
And Storage

Abstract Extracellular vesicles (EVs) are active components of red blood cell (RBC) concentrates and may be associated with beneficial and adverse effects of transfusion. Elucidating controllable factors associated with EV release in RBC products is thus important to better manage the quality and properties of RBC units. Erythrocyte-derived EVs (EEVs) and platelet-derived EVs (PEVs) were counted in 1226 RBC units (administered to 280 patients) using a standardized cytometry-based method. EV size and CD47 and annexin V expression were also measured. The effects of donor characteristics, processing methods, and storage duration on EV counts were analyzed by using standard comparison tests, and analysis of covariance was used to determine factors independently associated with EV counts. PEV as well as EEV counts were higher in whole-blood–filtered RBC units compared with RBC-filtered units; PEV counts were associated with filter type (higher with filters associated with higher residual platelets), and CD47 expression was higher on EEVs in RBC units stored longer. Multivariate analysis showed that EEV counts were strongly associated with filter type (P < .0001), preparation, and storage time (+25.4 EEV/µL per day [P = .01] and +42.4 EEV/µL per day [P < .0001], respectively). The only independent factor associated with PEV counts was the residual platelet count in the unit (+67.1 PEV/µL; P < .0001). Overall, processing methods have an impact on EV counts and characteristics, leading to large variations in EV quantities transfused into patients. RBC unit processing methods might be standardized to control the EV content of RBC units if any impacts on patient outcomes can be confirmed. The IMIB (Impact of Microparticles in Blood) study is ancillary to the French ABLE (Age of Transfused Blood in Critically Ill Adults) trial (ISRCTN44878718).

scholarly journals Microparticles from red blood cell transfusion products induce a strong inflammatory host response

Critical Care ◽  
2015 ◽  
Vol 19 (Suppl 1) ◽  
pp. P336
Author(s):  
M Straat ◽  
M Hezel ◽  
A Boing ◽  
R Nieuwland ◽  
R Bruggen ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Host Response ◽  
Red Blood Cell Transfusion

scholarly journals Red‐blood‐cell manufacturing methods and storage solutions differentially induce pulmonary cell activation

Vox Sanguinis ◽  
2020 ◽  
Vol 115 (5) ◽  
pp. 395-404 ◽  
Author(s):  
Mathijs R. Wirtz ◽  
Ruqayyah J. Almizraq ◽  
Nina C. Weber ◽  
Philip J. Norris ◽  
Suchitra Pandey ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Activation ◽  
Cell Manufacturing ◽  
Storage Solutions ◽  
Manufacturing Methods ◽  
And Storage

Effect of red blood cell storage duration on major postoperative complications in cardiac surgery: A randomized trial

2020 ◽  
Vol 160 (6) ◽  
pp. 1505-1514.e3 ◽  
Author(s):  
Colleen G. Koch ◽  
Daniel I. Sessler ◽  
Andra E. Duncan ◽  
Edward J. Mascha ◽  
Liang Li ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Postoperative Complications ◽  
Blood Cell ◽  
Randomized Trial ◽  
Red Blood Cell ◽  
Storage Duration ◽  
Cell Storage

Red blood cell (RBC) age at collection and storage influences RBC membrane-associated carbohydrates and lectin binding

Transfusion ◽  
2007 ◽  
Vol 47 (6) ◽  
pp. 966-968 ◽  
Author(s):  
Rosemary L. Sparrow ◽  
Margaret F. Veale ◽  
Geraldine Healey ◽  
Katherine A. Payne
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Lectin Binding ◽  
Rbc Membrane ◽  
And Storage

scholarly journals The impact of red blood cell storage duration on tissue oxygenation in cardiac surgery

2017 ◽  
Vol 153 (3) ◽  
pp. 610-619.e2 ◽  
Author(s):  
Christopher P. Stowell ◽  
Glenn Whitman ◽  
Suzanne Granger ◽  
Hernando Gomez ◽  
Susan F. Assmann ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Tissue Oxygenation ◽  
Storage Duration ◽  
The Impact ◽  
Cell Storage

scholarly journals Red blood cell-derived extracellular vesicles mediate intercellular communication in ischemic heart failure

2019 ◽  
Author(s):  
Avash Das ◽  
Nedyalka Valkov ◽  
Ane M. Salvador ◽  
Ivan Kur ◽  
Olivia Ziegler ◽  
...  
Keyword(s):  
Heart Failure ◽  
Mouse Model ◽  
Blood Cell ◽  
Extracellular Vesicles ◽  
Red Blood Cell ◽  
Significant Proportion ◽  
Ischemic Heart ◽  
Target Cells ◽  
Ischemic Heart Failure ◽  
Cellular Markers

SummaryExtracellular vesicles (EV) mediate intercellular signaling by transferring their cargo to recipient cells. Red blood cell (RBC)-derived EVs constitute a significant proportion of circulating EVs and have been implicated in regulating immune responses. Here, we describe a transgenic mouse model for fluorescent-based mapping of RBC-EV target cells based on the functional transfer of EV-contained Cre-recombinase to target cells. In a murine model of ischemic heart failure, we detect an increase in RBC-EV-targeted cardiomyocytes in the hearts and microglial cells in the brains. Cells targeted by RBC-EVs present an enrichment of genes implicated in cell proliferation and metabolism pathways compared to non-recombined (non-targeted) cells. Cardiomyocytes targeted by RBC-EVs are more likely to demonstrate cellular markers of DNA synthesis and proliferation, suggesting functional significance of EV-mediated signaling. In conclusion, we leverage our mouse model for mapping of RBC-EV targets in murine ischemic heart failure to demonstrate quantitative and qualitative changes in RBC-EV recipients.

scholarly journals Potential consequences of the red blood cell storage lesion on cardiac electrophysiology

2020 ◽  
Author(s):  
Marissa Reilly ◽  
Chantal Bruno ◽  
Tomas Prudencio ◽  
Nina Ciccarelli ◽  
Devon Guerrelli ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Large Volume ◽  
Cardiac Electrophysiology ◽  
Blood Products ◽  
Storage Duration ◽  
Storage Lesion ◽  
Biochemical Alterations ◽  
Cell Storage

AbstractThe red blood cell (RBC) storage lesion is a series of morphological, functional and metabolic changes that RBCs undergo following collection, processing and refrigerated storage for clinical use. Since the biochemical attributes of the RBC unit shifts with time, transfusion of older blood products may contribute to cardiac complications, including hyperkalemia and cardiac arrest. We measured the direct effect of storage age on cardiac electrophysiology and compared with hyperkalemia, a prominent biomarker of storage lesion severity. Donor RBCs were processed using standard blood banking techniques. The supernatant was collected from RBC units (sRBC), 7-50 days post-donor collection, for evaluation using Langendorff-heart preparations (rat) or human stem-cell derived cardiomyocytes. Cardiac parameters remained stable following exposure to ‘fresh’ sRBC (day 7: 5.9+0.2 mM K+), but older blood products (day 40: 9.7+0.4 mM K+) caused bradycardia (baseline: 279±5 vs day 40: 216±18 BPM), delayed sinus node recovery (baseline: 243±8 vs day 40: 354±23 msec), and increased the effective refractory period of the atrioventricular node (baseline: 77+2 vs day 40: 93+7 msec) and ventricle (baseline: 50+3 vs day 40: 98+10 msec) in perfused hearts. Beating rate was also slowed in human cardiomyocytes after exposure to older sRBC (−75+9%, day 40 vs control). Similar effects on automaticity and electrical conduction were observed with hyperkalemia (10-12 mM K+). This is the first study to demonstrate that ‘older’ blood products directly impact cardiac electrophysiology, using experimental models. These effects are likely due to biochemical alterations in the sRBC that occur over time, including, but not limited to hyperkalemia. Patients receiving large volume and/or rapid transfusions may be sensitive to these effects.New & noteworthyWe demonstrate that red blood cell storage duration time can have downstream effects on cardiac electrophysiology, likely due to biochemical alterations in the blood product. Hyperkalemia and cardiac arrest have been reported following blood transfusions, but this is the first experimental study to show a direct correlation between storage duration and cardiac function. Infant and pediatric patients, and those receiving large volume and/or rapid transfusions may be sensitive to these effects.

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