Bacterial Quorum Sensing Inhibition Activity of the Traditional Chinese Herbs, Ficus carica L. and Perilla frutescens

Chemotherapy ◽  
2014 ◽  
Vol 60 (5-6) ◽  
pp. 379-383 ◽  
Author(s):  
Shiwei Sun ◽  
Hui Li ◽  
Wanlong Zhou ◽  
Ao Liu ◽  
Hu Zhu
Keyword(s):  
Quorum Sensing ◽  
Perilla Frutescens ◽  
Chinese Herbs ◽  
Ficus Carica ◽  
Inhibition Activity ◽  
Quorum Sensing Inhibition ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum

scholarly journals Screening of Bacterial Quorum Sensing Inhibitors in a Vibrio fischeri LuxR-Based Synthetic Fluorescent E. coli Biosensor

2020 ◽  
Vol 13 (9) ◽  
pp. 263
Author(s):  
Xiaofei Qin ◽  
Celina Vila-Sanjurjo ◽  
Ratna Singh ◽  
Bodo Philipp ◽  
Francisco M. Goycoolea
Keyword(s):  
Quorum Sensing ◽  
Bacterial Growth ◽  
Vibrio Fischeri ◽  
Caffeic Acid Phenethyl Ester ◽  
Docking Studies ◽  
Inhibition Activity ◽  
E Coli ◽  
Bacterial Quorum Sensing ◽  
Pure Compounds ◽  
Bacterial Quorum

A library of 23 pure compounds of varying structural and chemical characteristics was screened for their quorum sensing (QS) inhibition activity using a synthetic fluorescent Escherichia coli biosensor that incorporates a modified version of lux regulon of Vibrio fischeri. Four such compounds exhibited QS inhibition activity without compromising bacterial growth, namely, phenazine carboxylic acid (PCA), 2-heptyl-3-hydroxy-4-quinolone (PQS), 1H-2-methyl-4-quinolone (MOQ) and genipin. When applied at 50 µM, these compounds reduced the QS response of the biosensor to 33.7% ± 2.6%, 43.1% ± 2.7%, 62.2% ± 6.3% and 43.3% ± 1.2%, respectively. A series of compounds only showed activity when tested at higher concentrations. This was the case of caffeine, which, when applied at 1 mM, reduced the QS to 47% ± 4.2%. In turn, capsaicin, caffeic acid phenethyl ester (CAPE), furanone and polygodial exhibited antibacterial activity when applied at 1mM, and reduced the bacterial growth by 12.8% ± 10.1%, 24.4% ± 7.0%, 91.4% ± 7.4% and 97.5% ± 3.8%, respectively. Similarly, we confirmed that trans-cinnamaldehyde and vanillin, when tested at 1 mM, reduced the QS response to 68.3% ± 4.9% and 27.1% ± 7.4%, respectively, though at the expense of concomitantly reducing cell growth by 18.6% ± 2.5% and 16% ± 2.2%, respectively. Two QS natural compounds of Pseudomonas aeruginosa, namely PQS and PCA, and the related, synthetic compounds MOQ, 1H-3-hydroxyl-4-quinolone (HOQ) and 1H-2-methyl-3-hydroxyl-4-quinolone (MHOQ) were used in molecular docking studies with the binding domain of the QS receptor TraR as a target. We offer here a general interpretation of structure-function relationships in this class of compounds that underpins their potential application as alternatives to antibiotics in controlling bacterial virulence.

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