scholarly journals Pttg1 Promotes Growth of Breast Cancer through P27 Nuclear Exclusion

2016 ◽  
Vol 38 (1) ◽  
pp. 393-400 ◽  
Author(s):  
Yishan Xiea ◽  
Rui Wangb
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Cell Apoptosis ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Nuclear Exclusion ◽  
Regulatory Machinery ◽  
Apoptosis Assay ◽  
Adjacent Normal Breast Tissue

Background/Aims: A role of Pituitary Tumor Transforming Gene 1 (Pttg1) in the carcinogenesis has been shown in some cancers, but not in BC (BC). Methods: We compared the levels of Pttg1 in the resected BC tissue with the adjacent normal breast tissue from the same patient. We modified Pttg1 levels in a BC cell line, MCF7, by either a Pttg1 transgene, or a Pttg1 shRNA. The cell growth was measured in an MTT assay. The cell apoptosis was measured by apoptosis assay. The nuclear protein of cell-cycle-related genes was examined in Pttg1-modifed BC cells. Co-immunoprecipitation was performed to examine the association of Pttg1 and p27. Results: We detected significantly higher levels of Pttg1 in the resected BC tissue, compared to the adjacent normal breast tissue from the same patient. Overexpression or depletion of Pttg1 in MCF7 significantly increased or inhibited cell growth, respectively. Changes in Pttg1 levels, however, did not alter cell apoptosis, suggesting that Pttg1 increases cell growth through augmented cell proliferation, rather than decreased cell apoptosis. Among all examined cell-cycle-related proteins in Pttg1-modifed BC cells, only nuclear p27 levels were significantly affected. Further, co-immunoprecipitation showed that Pttg1 directly associated with p27. Conclusion: Pttg1 may increase BC cell growth through nuclear exclusion of p27, which highlights a novel molecular regulatory machinery in tumorigenesis of BC.

scholarly journals MicroRNA-203 Regulates Growth and Metastasis of Breast Cancer

2015 ◽  
Vol 37 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Shan Zhao ◽  
Jinzhu Han ◽  
Likang Zheng ◽  
Zixin Yang ◽  
Li Zhao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Growth ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Cancer Cell Growth ◽  
Breast Cancer Cell Growth

Backgrounds/Aims: MicroRNAs (MiRNAs) control many biological events and play critical roles in the development of tumor. Among all miRNAs, miR203 has been recently shown to have an inhibitory effect on prostate cancer. However, its involvement in the carcinogenesis of breast cancer has not been reported. Methods: We examined the levels of miR203 in the breast cancer from the patients compared to the paired normal breast tissue. We also examined the levels of miR203 in several commonly used breast cancer cell lines. The effects of overexpression or depletion of miR203 on breast cancer cell growth were analyzed by a MTT assay, and on breast cancer cell invasion were examined by a scratch wound healing assay and a transwell cell migration assay. MiR203-targeted genes were analyzed by Western blot. Results: We detected significantly lower levels of miR203 in the breast cancer from the patients compared to the paired normal breast tissue. Moreover, the levels of miR203 were significantly lower in breast cancer tissue from the patients with cancer metastasis. Decreased miR203 levels were detected in all examined breast cancer lines. Overexpression of miR203 inhibited breast cancer cell growth and invasion, while antisense-mediated inhibition of miR203 enhanced cancer cell growth and invasion. Further analyses show that miR203 may inhibit cell growth through decreasing cell-cycle activator cyclinD2 and CDK6, increasing cell-cycle suppressor p21 and p27, and increasing apoptosis-associated protein Bcl-2. MiR203 may also inhibit cell metastasis through suppressing matrix metalloproteinase 2 (MMP2), MMP7 and MMP9. Conclusion: Our data thus highlight miR203 as a novel therapeutic target for breast cancer.

Promoter methylation and expression changes of CDH1 and P16 genes in invasive breast cancer and adjacent normal breast tissue

Neoplasma ◽  
2010 ◽  
Vol 57 (5) ◽  
pp. 465-472 ◽  
Author(s):  
A. Celebiler_Cavusoglu ◽  
A. Sevinc ◽  
S. Saydam ◽  
T. Canda ◽  
Z. Baskan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Invasive Breast Cancer ◽  
Promoter Methylation ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Adjacent Normal Breast Tissue

Differential expression of c-Met, its ligand HGF/SF and HER2/neu in DCIS and adjacent normal breast tissue

2007 ◽  
Vol 51 (1) ◽  
pp. 54-62 ◽  
Author(s):  
K Lindemann ◽  
J Resau ◽  
J Nährig ◽  
E Kort ◽  
B Leeser ◽  
...  
Keyword(s):  
Differential Expression ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Adjacent Normal Breast Tissue

scholarly journals Relationship between PD-L1 expression and clinical characteristics in patients with breast invasive ductal carcinoma

Open Medicine ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 288-292 ◽  
Author(s):  
Jian Lou ◽  
Yuefen Zhou ◽  
Jianhui Huang ◽  
Xiaojun Qian
Keyword(s):  
Invasive Ductal Carcinoma ◽  
Breast Tissue ◽  
Ductal Carcinoma ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Tumor Diameter ◽  
Pathological Characteristics ◽  
Breast Invasive Ductal Carcinoma ◽  
Positive Expression ◽  
Adjacent Normal Breast Tissue

AbstractObjectiveTo evaluate the expression of PD-L1 (programmed death 1 ligand 1, PD-L1) and its clinical significance in breast invasive ductal carcinoma.MethodsTumor samples were collected from 64 cases of breast invasive ductal carcinoma patients, and tumor adjacent normal breast tissue were obtained as normal control. The expression of PD-L1 were examined by immunohistochemical staining and real time PCR assay, its correlations with patients’ clinical pathological characteristics were analyzed.ResultsPD-L1 was found to be over-expressed in 24 of 64 (37.5%) breast invasive ductal carcinoma samples, while in 1 of 22 (4.5%) tumor adjacent normal breast tissue which indicated PD-L1 was higher expressed in breast invasive ductal carcinoma samples than the tumor adjacent normal breast tissue (P < 0.05). PD-L1 positive expression was associated with clinical pathological characteristics of TNM stage and pathology grading (P < 0.05). However, PD-L1 positive expression was not correlated with age (P > 0.05), menstruation status (P >0.05), family history of breast cancer (P > 0.05), tumor diameter (P > 0.05), lymph node metastasis (P > 0.05) and tumor location (P > 0.05).ConclusionPD-L1 may play an important role in invasive ductal carcinoma, which could be a potential indicator for advanced clinical stage and poor prognosis.

scholarly journals Expression Quantitative Trait loci (QTL) in tumor adjacent normal breast tissue and breast tumor tissue

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0170181 ◽  
Author(s):  
Alejandro Quiroz-Zárate ◽  
Benjamin J. Harshfield ◽  
Rong Hu ◽  
Nick Knoblauch ◽  
Andrew H. Beck ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Breast Tumor ◽  
Breast Tissue ◽  
Tumor Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Expression Quantitative Trait Loci ◽  
Breast Tumor Tissue ◽  
Adjacent Normal Breast Tissue
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