scholarly journals PU.1-Silenced Dendritic Cells Induce Mixed Chimerism and Alleviate Intestinal Transplant Rejection in Rats via a Th1 to Th2 Shift

2016 ◽  
Vol 38 (1) ◽  
pp. 220-228 ◽  
Author(s):  
Xingwei Xu ◽  
Xin Gao ◽  
Xiaofan Zhao ◽  
Yannian Liao ◽  
Wu Ji ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Transplant Rejection ◽  
Intestinal Transplantation ◽  
Mixed Chimerism ◽  
Brown Norway ◽  
Post Transplantation ◽  
Intestinal Transplant ◽  
End Stage ◽  
Graft Morphology ◽  
Norway Rats

Background/Aims: Intestinal transplantation is an effective treatment for end-stage bowel failure; however, graft rejection and the toxicity associated with non-specific immunosuppression are major limitations of this procedure. Studies have shown that mixed chimerism can produce post-transplantation immune tolerance. Here, we demonstrate that in rat intestinal transplantation, PU.1-silenced dendritic cells (DCs) plus bone marrow (BM) cell transfusion results in mixed chimerism, and we investigate the mechanisms responsible for the effects of mixed chimerism rejection. Methods: In a model of intestinal transplantation, male Brown Norway rats were the donors, and female Lewis rats were the recipients that were randomly divided into 4 groups: control, BM, BM-imDCs and BM-PU.1. The dynamic changes in graft morphology, rejection scoring and serum concentrations of Th1/Th2-related cytokines were investigated on postoperative days 0, 7, 14, 21, and 30. Results: The BM-PU.1 group had better graft health, milder pathologic injuries, and lower rejection grades compared with the other groups. The rates of mixed chimerism were significantly highest in the BM-PU.1 group and correlated with decreases in serum IL-2 and increases in serum IL-10. Conclusion: Transfusion of PU.1-silenced DCs and BM cells induces stable mixed chimerism and has the potential to reduce pathologic injuries via a pro-Th2 shift in the Th1/Th2 balance.

scholarly journals Effects of renal transplantation on erectile dysfunction: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Irham Arif Rahman ◽  
Nur Rasyid ◽  
Ponco Birowo ◽  
Widi Atmoko
Keyword(s):  
Systematic Review ◽  
Erectile Dysfunction ◽  
Kidney Transplantation ◽  
Renal Transplantation ◽  
End Stage Renal Disease ◽  
Erectile Function ◽  
Meta Analysis ◽  
Post Transplantation ◽  
Stage Renal Disease ◽  
End Stage

AbstractErectile dysfunction (ED) is a major global health burden commonly observed in patients with end-stage renal disease (ESRD). Although renal transplantation improves the problem in some patients, it persists in ≈20–50% of recipients. Studies regarding the effects of kidney transplantation on ED present contradictory findings. We performed a systematic review to summarise the effects of kidney transplantation on ED. A systematic literature search was performed across PubMed, Cochrane, and Scopus databases in April 2020. We included all prospective studies that investigated the pre and posttransplant international index of erectile function (IIEF-5) scores in recipients with ED. Data search in PubMed and Google Scholar produced 1326 articles; eight were systematically reviewed with a total of 448 subjects. Meta-analysis of IIEF-5 scores showed significant improvements between pre and post transplantation. Our findings confirm that renal transplantation improves erectile function. Furthermore, transplantation also increases testosterone level. However, the evidence is limited because of the small number of studies. Further studies are required to investigate the effects of renal transplantation on erectile function.

scholarly journals Aging leads to increased levels of protein O-linked N-acetylglucosamine in heart, aorta, brain and skeletal muscle in Brown-Norway rats

2008 ◽  
Vol 9 (3) ◽  
Author(s):  
Norbert Fülöp ◽  
Wenguang Feng ◽  
Dongqi Xing ◽  
Kai He ◽  
László G. Nőt ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Brown Norway ◽  
Norway Rats

Extending the Indications of Intestinal Transplantation - modified multivisceral transplantation for end-stage pseudomyxoma peritoneii

2017 ◽  
Vol 101 ◽  
pp. S89 ◽  
Author(s):  
Srikanth Reddy ◽  
Tom Cecil ◽  
Phil Allan ◽  
Vrakas Georgios ◽  
Ali Smith ◽  
...  
Keyword(s):  
Intestinal Transplantation ◽  
Multivisceral Transplantation ◽  
End Stage

scholarly journals Age-related declines in thirst and salt appetite responses in male Fischer 344×Brown Norway rats

2014 ◽  
Vol 135 ◽  
pp. 180-188 ◽  
Author(s):  
Robert L. Thunhorst ◽  
Terry Beltz ◽  
Alan Kim Johnson
Keyword(s):  
Brown Norway ◽  
Salt Appetite ◽  
Fischer 344 ◽  
Age Related ◽  
Norway Rats

Effect of interleukin-1β on airway hyperresponsiveness and inflammation in sensitized and nonsensitized Brown-Norway rats

1994 ◽  
Vol 93 (2) ◽  
pp. 464-469 ◽  
Author(s):  
Hideo Tsukagoshi ◽  
Tatsuo Sakamoto ◽  
Wenbing Xu ◽  
Peter J. Barnes ◽  
K.Fan Chung
Keyword(s):  
Airway Hyperresponsiveness ◽  
Interleukin 1Β ◽  
Brown Norway ◽  
Norway Rats

scholarly journals Calcium Metabolism following Renal Transplantation

1994 ◽  
Vol 31 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Anne M Straffen ◽  
DJS Carmichael ◽  
Angela Fairney ◽  
B Hulme ◽  
M Snell
Keyword(s):  
Renal Transplantation ◽  
Calcium Homeostasis ◽  
End Stage Renal Disease ◽  
Post Transplantation ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D ◽  
Transplant Patients ◽  
The Status ◽  
Stage Renal Disease ◽  
End Stage

Abnormalities of calcium homeostasis are a recognized feature of end-stage renal disease. The treatment of choice is renal transplantation, but this does not always result in normalization of the biochemical profile. Persistent hypercalcaemia is well documented and our study was undertaken to investigate the status of the calcium regulating hormones in renal patients post-transplantation. Serum calcium, parathyroid hormone, 1,25-dihydroxyvitamin D (1,25(OH)2D) and osteocalcin concentrations were measured in post-transplant patients. Twenty per cent of the patients had subnormal 1,25(OH)2D concentrations while 55% had biochemical evidence of hyperparathyroidism but only 5% were hypercalcaemic. Time elapsed since transplantation was not correlated with any of the analytes investigated and there was no relationship between persistent impairment of renal function and abnormalities of calcium homeostasis.

scholarly journals Enhanced Antigen-Specific Delayed-Type Hypersensitivity and Immunoglobulin G2b Responses after Oral Administration of ViableLactobacillus casei YIT9029 in Wistar and Brown Norway Rats

2001 ◽  
Vol 8 (4) ◽  
pp. 762-767 ◽  
Author(s):  
R. de Waard ◽  
J. Garssen ◽  
J. Snel ◽  
G. C. A. M. Bokken ◽  
T. Sako ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
Active Role ◽  
Lymphoid Organ ◽  
Delayed Type Hypersensitivity ◽  
Brown Norway ◽  
Infection Model ◽  
Cell Mediated Immunity ◽  
Th1 Cell ◽  
Inflammatory Reactions ◽  
Norway Rats

ABSTRACT In this study, the effects of orally administered viableLactobacillus casei Shirota strain YIT9029 on the immunity parameters of Wistar and Brown Norway rats were examined. For this purpose, we used the Trichinella spiralis host resistance model. Two weeks before and during T. spiralisinfection, rats were fed 109 viable L. casei bacteria 5 days per week. The T. spiralis-specific delayed-type hypersensitivity (DTH) response was significantly enhanced in both Wistar and Brown Norway rats given L. casei. In both rat strains fedL. casei, serum T. spiralis-specific immunoglobulin G2b (IgG2b) concentrations were also significantly increased. In the model, no significant effects ofL. casei on larval counts or inflammatory reactions in the tongue musculature, body weights, or lymphoid organ weights were observed. Serum specific antibody responses, other than IgG2b, were not changed by feeding of L. casei. In contrast toL. casei, it was shown that orally administeredBifidobacterium breve or Bifidobacterium bifidum had no influence on the measured infection and immunity indices in the rat infection model. Since the rat DTH response is considered to be a manifestation of Th1 cell-mediated immunity and the IgG2b isotype has been associated with Th1 activity, it was concluded that Th1 cells could play an active role in the immunomodulatory effects of orally administered L. casei. Furthermore, our data do not indicate that the effect of oral supplementation withL. casei is dependent on the genetic background of the host.

Pharmacological modulation of eosinophil influx into the lungs of Brown Norway rats

1996 ◽  
Vol 315 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Marian T. Namovic ◽  
Robin E. Walsh ◽  
Carole Goodfellow ◽  
Richard R. Harris ◽  
George W. Carter ◽  
...  
Keyword(s):  
Brown Norway ◽  
Pharmacological Modulation ◽  
Norway Rats
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