scholarly journals RNF43 Inhibits Cancer Cell Proliferation and Could be a Potential Prognostic Factor for Human Gastric Carcinoma

2015 ◽  
Vol 36 (5) ◽  
pp. 1835-1846 ◽  
Author(s):  
Lei Niu ◽  
Hong-Zhen Qin ◽  
Hong-Qing Xi ◽  
Bo Wei ◽  
Shao-You Xia ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Gastric Carcinoma ◽  
Tumor Formation ◽  
Cancer Prognosis ◽  
Depth Of Invasion ◽  
Regional Lymph Nodes ◽  
Gastric Carcinoma Cell ◽  
Normal Tissues ◽  
Gastric Carcinoma Cell Lines ◽  
Potential Prognostic Factor

Background/Aims: RNF43 is a member of transmembrane E3 ubiquitin ligases and plays important roles in tumor formation progression. In current study, we aimed to explore RNF43 expression and analyze its role in gastric carcinoma. Methods and Results: The level of RNF43 was detected in 77 cases of gastric carcinoma and matched normal tissues by real-time PCR, western blotting and immunohistochemistry. We found that the expression of RNF43 was significantly down-regulated in the gastric carcinoma tissues compared to the normal mucosae (all P<0.001). In addition, RNF43 was significantly correlated with histological differentiation (P = 0.001), T-stage cancer (P<0.001), depth of invasion (P<0.001), metastasis of regional lymph nodes (P<0.001), pTNM stage (P<0.001) and survival (P = 0.021). We further explored the biological functions of RNF43 in gastric carcinoma cell lines. Both gain- and loss-function assays show that RNF43 could suppress cell proliferation while promotes cell apoptosis. Further, we found that RNF43 was positively correlated with p53 and cleaved-caspase3 and negatively correlated with Ki67 and Lgr5. Concolusion: In conclusion, RNF43 might act as a tumor suppressor in gastric carcinoma and might be a potential indicator for the clinical assessment of gastric cancer prognosis

LncRNA HEIH promotes cell proliferation, migration and invasion by suppressing miR-214-3p in gastric carcinoma

2020 ◽  
Author(s):  
Lei Jiang ◽  
Luyao Zhang ◽  
Qian Chen ◽  
Shigang Qiao ◽  
Feng Zhou ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Gastric Carcinoma ◽  
Quantitative Polymerase Chain Reaction ◽  
Annexin V ◽  
Fluorescein Isothiocyanate ◽  
Cell Counting ◽  
Migration And Invasion ◽  
Gastric Carcinoma Cell ◽  
Normal Tissues ◽  
Apoptosis Detection

Abstract The present study aimed to investigate the function of long non-coding RNA HEIH in gastric carcinoma (GC). Adjacent normal tissues and GC tissues were obtained from 72 patients. Real-time quantitative polymerase chain reaction (RT-qPCR) was utilized to measure the expression of HEIH in cancer tissues and cells. Cell Counting Kit-8 and transwell assays were employed to evaluate cell proliferation, migration and invasion. An Annexin V-fluorescein-isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection Kit was used to evaluate the apoptosis ratio. RT-qPCR was used to detect the expression level of miR-214-3p. The expression of HEIH in GC tissues was higher than in adjacent normal tissues. The expression of HEIH was upregulated in MKN-45, NCL-N87, KATO III cell lines compared within normal gastric epithelial cells. Knockdown of lncRNA HEIH significantly decreased the number of migrated and invaded cells. Additionally, downregulation of HEIH could increase GC cell apoptosis compared with the non-specific control (NC) group. We also proved that miR-214-3p was the direct target of lncRNA HEIH, and that overexpression of miR-214-3p could reverse the effects of HEIH. Silencing of HEIH could suppress Gastric Carcinoma cell proliferation, migration and invasion by inhibiting miR-214-3p. Thus, HEIH might represent a novel biomarker and therapeutic target.

Mucins and mucin-associated carbohydrate antigens expression in gastric carcinoma cell lines

1999 ◽  
Vol 435 (5) ◽  
pp. 479-485 ◽  
Author(s):  
F. Carvalho ◽  
Leonor David ◽  
Jean-Pierre Aubert ◽  
Anna López-Ferrer ◽  
Carme De Bolós ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Gastric Carcinoma Cell ◽  
Carbohydrate Antigens ◽  
Carcinoma Cell Lines ◽  
Gastric Carcinoma Cell Lines

scholarly journals Sulforaphane suppresses autophagy during the malignant progression of gastric carcinoma via activating miR-4521/PIK3R3 pathway

2021 ◽  
pp. 096032712110544
Author(s):  
Zi-tan Peng ◽  
Pei Gu
Keyword(s):  
Cell Proliferation ◽  
Gastric Carcinoma ◽  
Carcinoma Cells ◽  
Malignant Progression ◽  
Gastric Carcinoma Cell ◽  
Over Expression ◽  
Anti Cancer ◽  
Underlying Mechanisms ◽  
Cruciferous Plants

Objective Sulforaphane, which exerts an effective anti-cancer ability, is a phytochemical converted from cruciferous plants. Here, we aimed to identify whether sulforaphane could suppress autophagy during the malignant progression of gastric carcinoma and to explore the underlying mechanisms. Methods SGC7901 cells were transfected with miR-4521 mimics, inhibitor, and pcDNA3.1- PIK3R3, and treated with sulforaphane or autophagy inhibitor. Cell proliferation, apoptosis, and miR-4521 or PIK3R3 expression were detected. Results MiR-4521 over-expression suppressed LC3-II/I ratio and Beclin-1 expression but induced p62 expression in SGC7901 cells. MiR-4521 also reduced gastric carcinoma cell proliferation and promoted apoptosis in vitro. In the mechanical observation, we identified that miR-4521 directly targeted PIK3R3 to repress its expression, and PIK3R3 up-regulation partly antagonized miR-4521-mediated autophagy, proliferation, and apoptosis in gastric carcinoma cells. In addition, sulforaphane exerted effective anti-cancer functions by repressing autophagy and growth in tumor cells at a concentration-dependent way. MiR-4521 inhibition or PIK3R3 over-expression weakened the anti-cancer functions of sulforaphane in gastric carcinoma cells. Conclusion Consequently, miR-4521 suppressed autophagy during the malignant progression of gastric carcinoma by targeting PIK3R3. Thus, miR-4521 may be applied as a therapeutic target for sulforaphane in gastric carcinoma.

scholarly journals Establishment and characterization of human gastric carcinoma cell lines

1997 ◽  
Vol 70 (4) ◽  
pp. 443-449 ◽  
Author(s):  
Jae-Gahb Park ◽  
Han-Kwang Yang ◽  
Woo Ho Kim ◽  
June-Key Chung ◽  
Myung-Soo Kang ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Gastric Carcinoma Cell ◽  
Human Gastric Carcinoma ◽  
Carcinoma Cell Lines ◽  
Gastric Carcinoma Cell Lines

Invasive potentials of gastric carcinoma cell lines: role of α 2 and α 6 integrins in invasion

1997 ◽  
Vol 123 (6) ◽  
pp. 310-316 ◽  
Author(s):  
Naoto Koike ◽  
Takeshi Todoroki ◽  
Hajime Komano ◽  
Tatsuro Shimokama ◽  
Shinichi Ban ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Gastric Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Gastric Carcinoma Cell Lines
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