15 Years� Experience with Hyperthermic Perfusion for Treatment of Soft Tissue Sarcoma and Malignant Melanoma of the Extremities

2015 ◽  
pp. 177-182 ◽  
Author(s):  
John S. Stehlin ◽  
Beppino C. Giovanella ◽  
Augusto E. Gutierrez ◽  
Peter D. de Ipolyi ◽  
Pierre J. Greeff
Keyword(s):  
Malignant Melanoma ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma

scholarly journals Granuloma Caused by Carbon Deposition in the Dermis

2014 ◽  
Vol 2014 ◽  
pp. 1-2
Author(s):  
Rintaro Shibuya ◽  
Yuichiro Endo ◽  
Akihiro Fujisawa ◽  
Miki Tanioka ◽  
Yoshiki Miyachi
Keyword(s):  
Computed Tomography ◽  
Foreign Body ◽  
Malignant Melanoma ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Foreign Body Reaction ◽  
Carbon Deposition ◽  
Case Reports ◽  
Initial Injury ◽  
Pencil Lead

Pencil core granuloma is characterized by a delayed foreign-body reaction against retained fragments of pencil lead. Previous case reports presented pencil core granuloma resembling malignant melanoma, haemangioma, or soft tissue sarcoma. We present a case of pencil core granuloma arising from the palm 25 years after the initial injury. The patient presented a bluish nodule that had been present over 25 years before. The nodule initially measured 5 mm in diameter. However, five years before presentation, it suddenly enlarged to the size of 30 mm during six months. Computed tomography (CT) of the lesion revealed a linear radiopaque structure of 8 mm long with a mass on its distal end. Surgical resection revealed a bluish muddy mass and pencil lead. Histological examination revealed degenerative tissue with calcification surrounded by massive amounts of black granular material in the middle and lower dermis.

Metastases of malignant melanoma simulating soft tissue sarcoma

1990 ◽  
Vol 417 (5) ◽  
pp. 377-388 ◽  
Author(s):  
Pär Lodding ◽  
Lars-Gunnar Kindblom ◽  
Lennart Angervall
Keyword(s):  
Malignant Melanoma ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma

Isolated Limb Perfusion for Malignant Melanoma and Soft Tissue Sarcoma

2003 ◽  
pp. 57-85
Author(s):  
Joseph M. Klausner ◽  
Dina Lev-Chelouche ◽  
Subhi Abu-Abeid ◽  
Mordechai Gutman
Keyword(s):  
Malignant Melanoma ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Isolated Limb Perfusion ◽  
Limb Perfusion

Isolated Limb Perfusion for Malignant Melanoma and Soft Tissue Sarcoma

2000 ◽  
pp. 57-85
Author(s):  
Joseph M. Klausner ◽  
Dina Lev-Chelouche ◽  
Subhi Abu-Abeid ◽  
Mordechai Gutman
Keyword(s):  
Malignant Melanoma ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Isolated Limb Perfusion ◽  
Limb Perfusion

Expression of Multidrug Resistance Genes MVP, MDR1, and MRP1 Determined Sequentially Before, During, and After Hyperthermic Isolated Limb Perfusion of Soft Tissue Sarcoma and Melanoma Patients

2002 ◽  
Vol 20 (15) ◽  
pp. 3282-3292 ◽  
Author(s):  
Ulrike Stein ◽  
Karsten Jürchott ◽  
Matthias Schläfke ◽  
Peter Hohenberger
Keyword(s):  
Multidrug Resistance ◽  
Malignant Melanoma ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Human Tumor ◽  
Transcriptional Level ◽  
Necrosis Factor Alpha ◽  
Mrna Induction ◽  
Limb Perfusion ◽  
Melanoma Patients

PURPOSE: Isolated, hyperthermic limb perfusion (ILP) with recombinant human tumor necrosis factor alpha and melphalan is a highly effective treatment for advanced soft tissue sarcoma (STS) and locoregional metastatic malignant melanoma. Multidrug resistance (MDR)-associated genes are known to be inducible by heat and drugs; expression levels of the major vault protein (MVP), MDR1, and MDR-associated protein 1 (MRP1) were determined sequentially before, during, and after ILP of patients. PATIENTS AND METHODS: Twenty-one STS or malignant melanoma patients were treated by ILP. Tumor tissue temperatures were recorded continuously and ranged from 33.4°C initially to peak values of 40.4°C during ILP. Serial true-cut biopsy specimens from tumor tissues were routinely microdissected. Expression analyses for MDR genes were performed by real-time reverse transcriptase polymerase chain reaction and immunohistochemistry. RESULTS: In 83% of the patients, MVP expression was induced during hyperthermic ILP. MVP-mRNA inductions often paralleled the increase in temperature during ILP. Increased MVP protein expressions either were observed simultaneously with the MVP-mRNA induction or were delayed until after the induction at the transcriptional level. Inductions of MDR1 and MRP1 were observed in only 13% and 27% of the specimens analyzed. Temperatures and drugs applied preferentially led to an induction of MVP and were not sufficient to induce MDR1 and MRP1 in the majority of tumors. CONCLUSION: This study is the first to analyze the expression of MDR-associated genes sequentially during ILP of patients and demonstrates that treatment might lead to increased levels of MVP, whereas enhanced levels of MDR1 and MRP1 remain rare events.

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

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