In vitro Cytotoxicity of Lymphocytes to Human Sarcoma Cells

2015 ◽  
pp. 846-851
Author(s):  
J. G. Sinkovics ◽  
J. R. Cabiness ◽  
C. C. Shullenberger
Keyword(s):  
In Vitro Cytotoxicity ◽  
Cytotoxicity Of Lymphocytes ◽  
Sarcoma Cells ◽  
Human Sarcoma

scholarly journals Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Simone Hettmer ◽  
Anna C Schinzel ◽  
Daria Tchessalova ◽  
Michaela Schneider ◽  
Christina L Parker ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Molecular Networks ◽  
Functional Genomic ◽  
Sarcoma Cell ◽  
Therapeutic Value ◽  
Current Therapies ◽  
Sarcoma Cells ◽  
Human Sarcoma

Current therapies for sarcomas are often inadequate. This study sought to identify actionable gene targets by selective targeting of the molecular networks that support sarcoma cell proliferation. Silencing of asparagine synthetase (ASNS), an amidotransferase that converts aspartate into asparagine, produced the strongest inhibitory effect on sarcoma growth in a functional genomic screen of mouse sarcomas generated by oncogenic Kras and disruption of Cdkn2a. ASNS silencing in mouse and human sarcoma cell lines reduced the percentage of S phase cells and impeded new polypeptide synthesis. These effects of ASNS silencing were reversed by exogenous supplementation with asparagine. Also, asparagine depletion via the ASNS inhibitor amino sulfoximine 5 (AS5) or asparaginase inhibited mouse and human sarcoma growth in vitro, and genetic silencing of ASNS in mouse sarcoma cells combined with depletion of plasma asparagine inhibited tumor growth in vivo. Asparagine reliance of sarcoma cells may represent a metabolic vulnerability with potential anti-sarcoma therapeutic value.

Ibandronate and Clodronate cause-dose and time- dependent apoptosis of human sarcoma cells in vitro

Bone ◽  
2006 ◽  
Vol 38 (3) ◽  
pp. 54
Author(s):  
A.A. Kurth ◽  
U. Stumpf ◽  
S. Goettig ◽  
M. Schoenherr ◽  
R. Henschler
Keyword(s):  
Time Dependent ◽  
Sarcoma Cells ◽  
Human Sarcoma

scholarly journals Further Studies of in Vitro Cytotoxicity of Lymphocytes for Colonic Epithelial Cells

1969 ◽  
Vol 57 (1) ◽  
pp. 30-35 ◽  
Author(s):  
R.G. Shorter ◽  
Martha Cardoza ◽  
S.G. ReMine ◽  
R.J. Spencer ◽  
K.A. Huizenga
Keyword(s):  
Epithelial Cells ◽  
In Vitro Cytotoxicity ◽  
Colonic Epithelial Cells ◽  
Cytotoxicity Of Lymphocytes

On the Role of Transferrin in 67Ga Uptake by Tumor Cells

1988 ◽  
Vol 27 (04) ◽  
pp. 151-153
Author(s):  
P. Thouvenot ◽  
F. Brunotte ◽  
J. Robert ◽  
L. J. Anghileri
Keyword(s):  
Specific Binding ◽  
Subcellular Fractionation ◽  
Animal Blood ◽  
Tumor Bearing ◽  
Cell Structures ◽  
The Difference ◽  
Sarcoma Cells ◽  
In Vitro Uptake

In vitro uptake of 67Ga-citrate and 59Fe-citrate by DS sarcoma cells in the presence of tumor-bearing animal blood plasma showed a dramatic inhibition of both 67Ga and 59Fe uptakes: about ii/io of 67Ga and 1/5o of the 59Fe are taken up by the cells. Subcellular fractionation appears to indicate no specific binding to cell structures, and the difference of binding seems to be related to the transferrin chelation and transmembrane transport differences

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