Demonstration of Helper Activity With Human Leukemic Tissue Extracts1

2015 ◽  
pp. 357-361
Author(s):  
R. A. Steeves ◽  
A. Fjelde ◽  
E. A. Mirand
Keyword(s):  
Helper Activity

scholarly journals Regulation of hematopoiesis II: the role of polyamine inhibition on helper or suppressor influences of the thymus

Blood ◽  
1983 ◽  
Vol 61 (3) ◽  
pp. 604-607 ◽  
Author(s):  
SJ Sharkis ◽  
GD Luk ◽  
MI Collector ◽  
PP McCann ◽  
SB Baylin ◽  
...  
Keyword(s):  
Suppressor Cells ◽  
Model Systems ◽  
Accessory Cell ◽  
Suppressor Activity ◽  
Polyamine Biosynthesis ◽  
Erythroid Progenitor ◽  
Rate Limiting Step ◽  
Helper Activity ◽  
Cell Subpopulations

Abstract We have previously suggested in murine model systems, that two cell subpopulations with differing proliferative capacity, from the thymus, modify the growth of erythroid progenitor cells in vitro. In order to further characterize these populations, we have specifically inhibited polyamine biosynthesis; this pathway is essential for the process of cell replication. Thus, alpha-difluoromethyl ornithine (DFMO) was used to block the conversion of ornithine to putrescine, the first and rate- limiting step in polyamine biosynthesis. We observed a threefold increase in hematopoietic progenitors (CFU-S and CFU-E) from bone marrow in animals treated with DFMO. We further examined the effect of DFMO on accessory “helper” and “suppressor” cells from the thymus and observed an increase in helper activity with an elimination of suppressor activity. All of these effects of DFMO were specific for inhibition of polyamine biosynthesis, since simultaneous addition of the depleted biosynthetic product, putrescine, restored suppressor activity. We conclude that polyamine biosynthesis is required acutely for accessory cell regulation of hematopoiesis.

scholarly journals Mice with the xid defect have helper cells for T15 idiotype-dominant anti-phosphorylcholine primary and secondary plaque-forming cells responses.

1982 ◽  
Vol 155 (4) ◽  
pp. 1245-1250 ◽  
Author(s):  
J Quintáns ◽  
Z S Quan ◽  
M A Arias
Keyword(s):  
T Cells ◽  
B Cells ◽  
Helper T Cells ◽  
Normal Female ◽  
Male And Female ◽  
Female Mice ◽  
Cell Responses ◽  
Helper Cells ◽  
Helper Activity

We have examined the abilities of helper T cells from commercially available (CBA/N X BALB/c)F1 (NBF1) xid male and phenotypically normal female mice to help T15+ and T15- B cells to produce thymus-dependent phosphorylcholine (PC)-specific direct plaque-forming cell responses. Carrier-primed T cells from both male and female mice were found (a) to restore T15+ TD responses in congenitally athymic BALB/c mice, (b) to help PC-primed BALB/c splenic B cells produce predominantly T15+ responses, and (c) to provide help for T15+ and T15- PFC responses generated by PC-primed normal F1 splenic B cells. Furthermore, carrier-primed irradiated xid and normal recipients contributed adequate helper activity for T15 dominant responses. We therefore conclude that male and female NBF1 mice are equally capable of helping T15+ responses.

An avian leukosis virus related to RSV(O): Properties and evidence for helper activity

Virology ◽  
1971 ◽  
Vol 43 (1) ◽  
pp. 223-234 ◽  
Author(s):  
Peter K. Vogt ◽  
Robert R. Friis
Keyword(s):  
Avian Leukosis Virus ◽  
Helper Activity

Human T Cell Hybridomas with Tetanus-Toxoid-Specific Helper Activity

1982 ◽  
pp. 191-201
Author(s):  
E. C. DeFreitas ◽  
S. Vella ◽  
A. Linnenbach ◽  
C. M. Croce ◽  
H. Koprowski
Keyword(s):  
T Cell ◽  
Tetanus Toxoid ◽  
Human T Cell ◽  
Helper Activity

scholarly journals Functional analysis of the murine T lymphocyte immune response to a protozoan parasite, Leishmania tropica

1983 ◽  
Vol 78 (1) ◽  
pp. 105-120 ◽  
Author(s):  
H. D. Engers ◽  
S. G. Coutinho ◽  
G. M. C. de Araújo Lima ◽  
J. A. Louis
Keyword(s):  
T Cell ◽  
Protozoan Parasite ◽  
Delayed Type Hypersensitivity ◽  
Cell Populations ◽  
Leishmania Tropica ◽  
Intracellular Parasites ◽  
Helper Activity ◽  
Cellular Immune ◽  
Parasite Leishmania

The results presented in this review summarize a seirs of experiments designed to characterize the murine T cell imune response to the protozoan parasite Leishmania tropica. Enriched T cell populations and T cell clones specific for L. tropica antigens were derived from lymph nodes of primed mice and maintained in continous culture in vitro. These T lymphocytes were shown (A) to express the Lyt 1+ 3- cell surface phenotype, (B) to proliferate specifically in vitro in response to parasite antigens, together with a source of irradiated syngeneic macrophages, (C) to transfer antigen-specific delayed-type hypersensitivity (DTH) responses to normal syngeneic mice, (D) to induce specific activation of parasitized macrophages in vitro resulting in the destruction of intracellular parasites, (E) to provide specific helper activity for antibody responses in vitro in a hapten-carrier system. Protection studies using these defiened T cell populations should allow the characterization of parasite antigen(s) implicated in the induction of cellular immune responses beneficial for the host.

scholarly journals Antigen-specific T lymphocyte clones. I. Characterization of a T lymphocyte clone expressing antigen-specific suppressive activity.

1981 ◽  
Vol 153 (5) ◽  
pp. 1246-1259 ◽  
Author(s):  
M Fresno ◽  
G Nabel ◽  
L McVay-Boudreau ◽  
H Furthmayer ◽  
H Cantor
Keyword(s):  
T Cell ◽  
T Lymphocyte ◽  
Antigen Binding ◽  
Suppressive Activity ◽  
Cell Functions ◽  
Cell Clones ◽  
Helper Activity ◽  
Binding Peptides ◽  
Lymphocyte Clone

We have generated continuously propagatable T lymphocyte clones to study antigen-specific T cell functions. All Ly-2+ clones mediate suppressive activity and secrete a characteristic pattern of polypeptides that differs from Ly-2- T cell clones. Cells of one clone, Cl.Ly23/4, specifically bind glycophorin from sheep erythrocytes (SRBC). After incubation with [35S]methionine, supernate material from this clone also contains biosynthetically labeled 70,000-mol wt proteins that specifically bind to SRBC and this binding is inhibited by glycophorin from sheep but not other erythrocytes. These antigen-binding 70,000-mol wt peptides specifically and completely suppress primary anti-SRBC responses generated by mixtures of primed Ly-1+2- cells and B cells. Suppression by these antigen-binding peptides reflects direct inhibition of T-helper activity.

scholarly journals An idiotype-specific helper population that bears immunoglobulin, Ia, and Lyt-1 determinants.

1984 ◽  
Vol 159 (4) ◽  
pp. 1189-1200 ◽  
Author(s):  
D H Sherr ◽  
M E Dorf
Keyword(s):  
T Cells ◽  
B Cells ◽  
Cell Population ◽  
T Helper Cells ◽  
T Helper ◽  
Phenotypic Characteristics ◽  
Surface Phenotype ◽  
Specific Recognition ◽  
Helper Cells ◽  
Helper Activity

A helper cell population with phenotypic characteristics of both B and T cells is described. This helper population, called BH, is present in normal unprimed C57BL/6 mice and preferentially helps the expression of NPb idiotype-bearing plaque-forming B cells in the absence of T helper cells. Its surface phenotype is Lyt-1.2+, Ig+, Lyb-3+, Thy-1.2-, Lyt-2.2-. The helper activity of the BH population is IgH restricted and BH cells selectively bind NPb idiotypic determinants. Collectively the data demonstrate that this unique subpopulation can regulate the response of antibody-secreting B cells through specific recognition of idiotypic determinants.

scholarly journals Early Development in the Peritoneal Cavity of CD49dhigh Th1 Memory Phenotype CD4+ T Cells with Enhanced B Cell Helper Activity

2015 ◽  
Vol 195 (2) ◽  
pp. 564-575 ◽  
Author(s):  
Hana Moon ◽  
Chanho Park ◽  
Jae-Ghi Lee ◽  
Sang Hyuck Shin ◽  
Joo Hee Lee ◽  
...  
Keyword(s):  
T Cells ◽  
B Cell ◽  
Early Development ◽  
Peritoneal Cavity ◽  
Cd4 T Cells ◽  
Memory Phenotype ◽  
Helper Activity
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