A New Differentiation Method of Cytotoxic Antibodies against Leucocytes

2015 ◽  
pp. 307-308
Author(s):  
Halina Seyfried ◽  
Barbara Maczynska-Rusiniak
Keyword(s):  
Cytotoxic Antibodies ◽  
Differentiation Method

Development of an immunochemical differentiation method for Salvia divinorum

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
MK Paudel ◽  
O Shirota ◽  
S Sekita ◽  
H Tanaka ◽  
S Morimoto
Keyword(s):  
Salvia Divinorum ◽  
Differentiation Method

scholarly journals An Efficient Method for the Differentiation of Human iPSC-Derived Endoderm toward Enterocytes and Hepatocytes

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 812
Author(s):  
Shimeng Qiu ◽  
Yaling Li ◽  
Yuki Imakura ◽  
Shinji Mima ◽  
Tadahiro Hashita ◽  
...  
Keyword(s):  
Small Intestine ◽  
Activin A ◽  
Double Positive ◽  
Differentiation Method ◽  
Drug Metabolizing Enzyme ◽  
Human Ipscs ◽  
Novel Method ◽  
Induced Pluripotent ◽  
Metabolizing Enzyme ◽  
Human Ipsc

The endoderm, differentiated from human induced pluripotent stem cells (iPSCs), can differentiate into the small intestine and liver, which are vital for drug absorption and metabolism. The development of human iPSC-derived enterocytes (HiEnts) and hepatocytes (HiHeps) has been reported. However, pharmacokinetic function-deficiency of these cells remains to be elucidated. Here, we aimed to develop an efficient differentiation method to induce endoderm formation from human iPSCs. Cells treated with activin A for 168 h expressed higher levels of endodermal genes than those treated for 72 h. Using activin A (days 0–7), CHIR99021 and PI−103 (days 0–2), and FGF2 (days 3–7), the hiPSC-derived endoderm (HiEnd) showed 97.97% CD−117 and CD−184 double-positive cells. Moreover, HiEnts derived from the human iPSC line Windy had similar or higher expression of small intestine-specific genes than adult human small intestine. Activities of the drug transporter P-glycoprotein and drug-metabolizing enzyme cytochrome P450 (CYP) 3A4/5 were confirmed. Additionally, Windy-derived HiHeps expressed higher levels of hepatocyte- and pharmacokinetics-related genes and proteins and showed higher CYP3A4/5 activity than those derived through the conventional differentiation method. Thus, using this novel method, the differentiated HiEnts and HiHeps with pharmacokinetic functions could be used for drug development.

A quantitative differentiation method for plastic bags by wide angle X-ray diffraction for tracing the source of illegal drugs

2007 ◽  
Vol 168 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Valerio Causin ◽  
Carla Marega ◽  
Pietro Carresi ◽  
Sergio Schiavone ◽  
Antonio Marigo
Keyword(s):  
Illegal Drugs ◽  
Wide Angle ◽  
X Ray Diffraction ◽  
X Ray ◽  
Differentiation Method ◽  
Plastic Bags

Differentiation of Human Motion Data Using Combined Spline and Least Squares Concepts

1991 ◽  
Vol 113 (3) ◽  
pp. 348-351 ◽  
Author(s):  
W. Simons ◽  
K. H. Yang
Keyword(s):  
Least Squares ◽  
Human Motion ◽  
Smoothing Technique ◽  
Data Smoothing ◽  
Motion Data ◽  
Differentiation Method ◽  
Benchmark Data

A differentiation method, which combines the concepts of least squares and splines, has been developed to analyze human motion data. This data smoothing technique is not dependent on a choice of a cut-off frequency and yet it closely reflects the nature of the phenomenon. Two sets of published benchmark data were used to evaluate the new algorithm.

CYTOTOXIC ANTIBODIES IN SERUM OF PREGNANT WOMEN AT DELIVERY

2009 ◽  
Vol 79B (4) ◽  
pp. 595-598
Author(s):  
Gunnar Nymand ◽  
Iver Heron ◽  
Kaj Gert Jensen ◽  
Anne Lundsgaard
Keyword(s):  
Pregnant Women ◽  
Cytotoxic Antibodies
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