Frequency of the Mg Blood Group Antigen in Swiss Blood Donors and Its Inheritance in Several Independent Families

2015 ◽  
pp. 206-209 ◽  
Author(s):  
M. N. Metaxas ◽  
M. Matter ◽  
M. Metaxas-B�hler ◽  
Y. Romanski ◽  
A. H�ssig
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigen ◽  
Independent Families

scholarly journals Prevalence of Weak D (Du) in Blood Donors in a Referral Teaching Hospital

2017 ◽  
Vol 6 (2) ◽  
pp. 1509
Author(s):  
H. S. Lamba ◽  
Kulbir Kaur ◽  
Kulwant Kaur ◽  
Amarjit Singh Vij
Keyword(s):  
Patient Care ◽  
Blood Group ◽  
Teaching Hospital ◽  
Blood Donors ◽  
Blood Product ◽  
Blood Group Antigen ◽  
Abo Blood Group ◽  
Blood Samples ◽  
Donor Population ◽  
Gel Technique

<p><strong>Background:</strong> Rh D is the most important Blood Group antigen after ABO Blood group antigen for transfusion purpose. All negative blood units by routine methods must be tested to detect weak D using IAT method. When the test for D and D<sup>u</sup> is positive, the label should read Rh(D) Positive . When the test for D and D<sup>u</sup> is negative, the label should read Rh(D) Negative.</p><p><strong>Objective:</strong> To know the prevalence of weak D in the donor population. No study has been done in this part of the country earlier. It will help in the knowledge of weak D, which is very important for better patient care and prevent allo-immunzation in blood recipients.</p><p><strong>Materials and Methods:</strong> Blood samples were tested by ID Gel technique or by tube method with two anti D reagents - anti-D IgM monoclonal and blend of anti-D IgM&amp;amp;IgG. All negative samples were further tested for weak D in IAT phase by LISS/Coombs' gel card.</p><p><strong>Results:</strong> A total of 13043 samples were tested from January 2011 to December 2013. 12196 were Rh positive and 847 were Rh D negative. Weak D was positive in 8 samples.</p><p><strong>Conclusion:</strong> The study shows the prevalence of weak D as 0.07% in blood donors who were primarily from in and around Jalandhar in Punjab. These donors may have posed problem to the recipients of blood and blood product and their detection prevented them from alloimunisation.</p>

scholarly journals A point mutation in the GYPC gene results in the expression of the blood group Ana antigen on glycophorin D but not on glycophorin C: further evidence that glycophorin D is a product of the GYPC gene

Blood ◽  
1993 ◽  
Vol 82 (10) ◽  
pp. 3198-3203
Author(s):  
G Daniels ◽  
MJ King ◽  
ND Avent ◽  
G Khalid ◽  
M Reid ◽  
...  
Keyword(s):  
Amino Acid ◽  
Blood Group ◽  
Blood Donors ◽  
Antigen Expression ◽  
Protein Product ◽  
Blood Group Antigen ◽  
Amino Acid Residues ◽  
The Common ◽  
Polymerase Chain ◽  
Glycophorin C

Glycophorin C (GPC) and glycophorin D (GPD) are homologous sialoglycoproteins in the human red blood cell membrane. Both are thought to be encoded by the GPC gene (GYPC). We report that the rare blood group antigen, Ana, is expressed on GPD but not on GPC. cDNA was synthesized from total RNA obtained from two unrelated, heterozygous Ana+ blood donors and analyzed by the polymerase chain reaction using primers that spanned sequences encoded by the GYPC gene. The expected 412-bp fragment was generated, and sequencing of the amplified product showed a G-->T substitution at nucleotide 67 of the coding sequence, resulting in the substitution of alanine by serine at amino acid residue 23 of GPC and, presumably, residue 2 of GPD. To explain the expression of Ana on GPD but not on GPC, we postulate that the conformation of the amino acid residues at the N-terminal region of GPD determines the antigenic expression as this conformation would be different from that of the same sequence of amino acids occurring within GPC. Other possible reasons for antigen expression on a shorter protein product but not on the full-length protein product of the same gene are discussed. We extrapolate this reasoning to account for the expression of the common GE2 blood group antigen on GPD but not on GPC.

scholarly journals Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Divjot Singh Lamba ◽  
Ravneet Kaur ◽  
Sabita Basu
Keyword(s):  
Racial Differences ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigen ◽  
Blood Group System ◽  
Blood Group Antigens ◽  
Young Females ◽  
Group System ◽  
Clinically Significant ◽  
Antigen Frequency

Background. Racial differences in blood group antigen distribution are common and may result in striking and interesting findings. These differences in blood group antigen distribution are important due to their influence on the clinical practice of transfusion medicine.Study Design and Methods. This is a prospective study, involving 1000 healthy regular repeat voluntary blood donors associated with the department. The clinically significant minor blood group antigens of these donors were studied.Results. Out of 1000 healthy regular repeat voluntary blood donors, 93% were D positive and 2.8% were K positive. Amongst the Rh antigens, e was the most common (99%), followed by D (93%), C (85.1%), c (62.3%), and E (21.5%). Within the MNS blood group system, antigen frequency was M (88%), N (57.5%), S (57.8%), and s (87.5%). Within the Duffy blood group system, antigen frequency wasFya(87.3%) andFyb(58.3%).Conclusions. This data base will help us to prevent alloimmunisation in young females, pregnant women, and patients who are expected to require repeated transfusions in life by providing them with antigen matched blood. Antigen negative blood can also be made available without delay to already alloimmunized multitransfused patients.

scholarly journals Discrepancies Between SNPs and Serology in Calling Blood Types in Asian and Native American Blood Donors

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1552-1552
Author(s):  
Jill M Johnsen ◽  
Gayle T Teramura ◽  
Samantha Harris ◽  
Meghan Delaney
Keyword(s):  
Genetic Variation ◽  
Native American ◽  
Ethnic Groups ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigen ◽  
Blood Type ◽  
Blood Group Antigens ◽  
Gene Variants ◽  
Blood Types

Abstract Introduction: Blood types result from genetic variation at blood group genes which directs the expression of blood group antigens on red blood cells. Determination of blood types is clinically important, as blood type mismatches can instigate potentially life-threatening allo-immune responses in transfusion recipients, transplant patients, and pregnant women. Blood types present in Asian American and Native American populations but rare or absent in the blood donor inventory, which is largely European American, can pose significant challenges to effective transfusion and pre-transfusion testing. We sought to more deeply characterize the blood type diversity present in Asian and Native Americans. Methods: Consenting blood donors self-identified to be of Asian or Native American descent were eligible. Conventional serologic methods were used determine C, Jka, Jkb, M, and N blood types. Genotyping was performed with a blood type SNP array (HEA Beadchip™, BioArray Solutions) to genetically assign c, C, e, E, K, k, Kpa, Kpb, Jsa, Jsb, Jka, Jkb, Fya, Fyb, , M, N, S, s, Lua, Lub, Dia, Dib, Coa, Cob, Doa, Dob, Joa, Hy, LWa, LWb, Sc1, and Sc2. For both methods, blood type was designated as positive “+” (consistent with presence of a blood group antigen) or negative “0” (consistent with absence of a blood group antigen). A SNP-serology discrepancy was defined as a “+” assignment by one method and a “0” by the other. Ambiguous SNP determinations were called “+” or “0” based upon relative signal intensities using established algorithms whenever possible; if not possible, the SNP-determined blood types were designated either inconclusive (IC) or low signal (LS). Results: A total of 8454 Asian and Native American blood donors representing 9 distinct ethnic groups were included in the study. As expected, the frequencies of rare and uncommon blood types differed between ethnic groups and in comparison to Europeans. Overall, 4.7% of blood donors studied exhibited one or more SNP-serology discrepancies for the four blood types tested by both methods (Table 1). The frequency of discrepancies was widely variable between blood types and between study populations (Figure 1), reaching as high as 5.4% for N (in Southeast Asians) and 7.2% for Jkb (in Pacific Islander/Hawaiians). We additionally observed patterns in IC and LS calls which varied between blood types and between ethnicities, suggesting that underlying genetic variation may contribute to inconclusive or low signal SNP results. Conclusions: We characterized 8454 Asian and Native American blood donors for blood type by serology and with SNPs. As expected, we observed variation in the frequencies of blood type SNPs both between study populations and in comparison to Europeans. With additional testing, we found that 4.7% of donors exhibited discrepancies between SNP-predicted and serology-detected blood type, and that the frequencies of discrepancies varied between ethnic groups. We hypothesize that clinically relevant blood group gene variants were not accurately predicted using this SNP approach due to underlying genetic diversity at blood group loci in these populations. We propose that a more comprehensive approach, such as DNA sequencing, would characterize blood group gene variants in individuals of Asian and Native American heritage, as well as other genetically diverse populations. Disclosures No relevant conflicts of interest to declare.

scholarly journals Blood group antigen distribution in Lao blood donors

2019 ◽  
Vol 28 (4) ◽  
pp. 132-136
Author(s):  
Chirapha Keokhamphoui ◽  
Yupa Urwijitaroon ◽  
Douangchanh Kongphaly ◽  
Te Thammavong
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigen

scholarly journals A point mutation in the GYPC gene results in the expression of the blood group Ana antigen on glycophorin D but not on glycophorin C: further evidence that glycophorin D is a product of the GYPC gene

Blood ◽  
1993 ◽  
Vol 82 (10) ◽  
pp. 3198-3203 ◽  
Author(s):  
G Daniels ◽  
MJ King ◽  
ND Avent ◽  
G Khalid ◽  
M Reid ◽  
...  
Keyword(s):  
Amino Acid ◽  
Blood Group ◽  
Blood Donors ◽  
Antigen Expression ◽  
Protein Product ◽  
Blood Group Antigen ◽  
Amino Acid Residues ◽  
The Common ◽  
Polymerase Chain ◽  
Glycophorin C

Abstract Glycophorin C (GPC) and glycophorin D (GPD) are homologous sialoglycoproteins in the human red blood cell membrane. Both are thought to be encoded by the GPC gene (GYPC). We report that the rare blood group antigen, Ana, is expressed on GPD but not on GPC. cDNA was synthesized from total RNA obtained from two unrelated, heterozygous Ana+ blood donors and analyzed by the polymerase chain reaction using primers that spanned sequences encoded by the GYPC gene. The expected 412-bp fragment was generated, and sequencing of the amplified product showed a G-->T substitution at nucleotide 67 of the coding sequence, resulting in the substitution of alanine by serine at amino acid residue 23 of GPC and, presumably, residue 2 of GPD. To explain the expression of Ana on GPD but not on GPC, we postulate that the conformation of the amino acid residues at the N-terminal region of GPD determines the antigenic expression as this conformation would be different from that of the same sequence of amino acids occurring within GPC. Other possible reasons for antigen expression on a shorter protein product but not on the full-length protein product of the same gene are discussed. We extrapolate this reasoning to account for the expression of the common GE2 blood group antigen on GPD but not on GPC.

ABO and Rhesus (D) Blood Group Distribution among Blood Donors in Rural South Haryana (Mewat-Region): A 5 Years Retrospective Study

2018 ◽  
Vol 4 (3) ◽  
Author(s):  
Sudheer Singh ◽  
Shailesh Kumar Mishra ◽  
Shivani Kalhan ◽  
Puja Sharma ◽  
Rahul N Satarkar ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Blood Group ◽  
Blood Donors ◽  
Rural South ◽  
Group Distribution

A Weak Example of the Blood Group Antigen A

Vox Sanguinis ◽  
1961 ◽  
Vol 6 (2) ◽  
pp. 151-156 ◽  
Author(s):  
B. P. L. Moore ◽  
P. H. Newstead ◽  
Joanne Johnson
Keyword(s):  
Blood Group ◽  
Blood Group Antigen ◽  
Antigen A

Studies on the Blood Group Antigen Me

Vox Sanguinis ◽  
1966 ◽  
Vol 11 (2) ◽  
pp. 157-169
Author(s):  
M.N. Metaxas ◽  
M. Metaxas-Bühler ◽  
J. Romanski
Keyword(s):  
Blood Group ◽  
Blood Group Antigen
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