The Role of Fresh Serum Factors in the Sensitization of Red Cells by Isoantibodies

2015 ◽  
pp. 502a-505
Author(s):  
F. Stratton
Keyword(s):  
Red Cells ◽  
Serum Factors ◽  
Fresh Serum

scholarly journals Role of Na+ in the Transport of Amino Acids in Rabbit Red Cells

1967 ◽  
Vol 242 (7) ◽  
pp. 1450-1457
Author(s):  
Kenneth P. Wheeler ◽  
Halvor N. Christensen
Keyword(s):  
Amino Acids ◽  
Red Cells

Myocardial CO2 buffering: role of transmembrane transport of H+ or HCO3-ions

1976 ◽  
Vol 230 (4) ◽  
pp. 1037-1041 ◽  
Author(s):  
DR Strome ◽  
RL Clancy ◽  
NC Gonzalez
Keyword(s):  
Small Volume ◽  
Coronary Circulation ◽  
Myocardial Cell ◽  
Ringer Solution ◽  
Red Cells ◽  
Transmembrane Transport ◽  
High Degree

Isolated rabbit hearts were perfused with rabbit red cells suspended in Ringer solution. A small volume of perfusate was recirculated for 10 min at Pco2 of 33.4 +/- 0.9 or 150.8 +/- 7.5 mmHg. Hypercapnia resulted in an increase in perfusate HCO3- concentration that was smaller than that observed when isolated perfusate was equilibrated in vitro with the same CO2 tensions (delta HCO-3e = 1.6 mM, P less than 0.01). This difference is consistent with a net movement of HCO3- into or H+ out of the mycardial cell, and cannot be accounted for by dilution of HCO3- in the myocardial interstitium. Recirculation of perfusate through the coronary circulation at normal Pco2 for two consecutive 10-min periods was not followed by changes in perfusate HCO3- concentration. A high degree of correlation (r = 0.81) was observed between intracellular HCO-3e concentration and the corresponding delta HCO-3e in individual experiments. The results suggest that transmembrane exchange of H+ or HCO3- is a buffer mechanism for CO2 in the myocardial cell.

scholarly journals THE ENHANCEMENT OF BACTERIAL PHAGOCYTOSIS BY SERUM

1969 ◽  
Vol 129 (6) ◽  
pp. 1275-1290 ◽  
Author(s):  
Richard B. Johnston ◽  
Martin R. Klemperer ◽  
Chester A. Alper ◽  
Fred S. Rosen
Keyword(s):  
Normal Serum ◽  
Phagocytic Index ◽  
Peptidase Activity ◽  
Complement Components ◽  
Serum Factors ◽  
Heat Labile ◽  
Dye Reduction ◽  
Sequential Addition ◽  
Enzymatic Inactivation

The role of serum factors in the phagocytosis of pneumococci was studied employing a spectrophotometric assay which measures reduced nitro blue tetrazolium (NBT) dye. Dye reduction occurs within the phagocyte shortly after bacterial ingestion as measured by the phagocytic index technique and by the uptake of 125I-pneumococci. Bacteria prepared with γG antibody were not phagocytosed unless a small volume of fresh normal serum was added. Using fresh sera deficient in single complement components, it was demonstrated that the first four components are necessary for optimal bacterial phagocytosis. When highly purified complement components were added to the antibody-coated pneumococci, enhancement of phagocytosis was achieved only with the sequential addition of C1, C4, C2, and C3. Evidence has been presented that human C3 bound to an immune complex exhibits peptidase activity and that this activity is essential for phagocytosis. A heat-labile, dialyzable serum cofactor which enhances C3 peptidase activity enhanced the phagocytosis of pneumococci prepared with purified complement components. A second phagocytosis-promoting cofactor, which is not a complement component, was found to be a heat-labile, 5–6S, beta pseudoglobulin. This protein may stabilize C3 peptidase activity or inhibit enzymatic inactivation of C3.

pH environmental of red cells in the spleen

1976 ◽  
Vol 231 (6) ◽  
pp. 1672-1678 ◽  
Author(s):  
MJ Levesque ◽  
AC Groom
Keyword(s):  
Splenic Tissue ◽  
Red Cells ◽  
Red Cell ◽  
Cat Spleen ◽  
Flow Through ◽  
Red Pulp ◽  
Reduced Flow

Intrasplenic pH in vivo was deduced from measurements on blood drained from cat spleen during contraction with the inflow occluded. The pH of blood in the red pulp is normally 7.20, but stasis or reduced flow through the pulp causes pH to fall toward 6.8. The splenic pulp contains blood of high hematocrit. To evaluate the role of buffering by the red cells themselves, intrasplenic p/ in red cell-free spleens was, therefore, estimated atering and leaving the spleen during red cell washout. At inflow pH less than 6.8 the outflow pH was raised, at inflow pH = 6.8 there was no change, b,t at inflow pH greater than 6.8 the outflow pH was lowered. These results indicate that the pH environment of red cells in the spleen results indicate that the pH environment of red cells in the spleen results from the interplay of two separate factors: i) pH-determining elements of the splenic tissue that buffer at 6.8, and ii) buffering provided by red cells passing through the pulp.

Some Comments on the Role of Blocking Serum Factors in Tolerance to Allografts

1972 ◽  
pp. 314-316
Author(s):  
Ingegerd Hellström ◽  
Karl Erik Hellström
Keyword(s):  
Serum Factors

scholarly journals THE ROLE OF OXYGEN IN THE REGULATION OF ERYTHROPOIESIS. DEPRESSION OF THE RATE OF DELIVERY OF NEW RED CELLS TO THE BLOOD BY HIGH CONCENTRATIONS OF INSPIRED OXYGEN 12

1949 ◽  
Vol 28 (6 Pt 2) ◽  
pp. 1544-1564 ◽  
Author(s):  
John C. Tinsley ◽  
Carl V. Moore ◽  
Reubenia Dubach ◽  
Virginia Minnich ◽  
Moises Grinstein
Keyword(s):  
Red Cells ◽  
High Concentrations ◽  
Inspired Oxygen

scholarly journals Toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors

2012 ◽  
Vol 72 (8) ◽  
pp. 1382-1389 ◽  
Author(s):  
Marzena Ciechomska ◽  
Christiaan A Huigens ◽  
Thomas Hügle ◽  
Tess Stanly ◽  
Andreas Gessner ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Toll Like Receptor ◽  
Serum Factors ◽  
Enhanced Production
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