Dual Processes of Desensitization and Resensitization Regulate Cellular Responsiveness in Smooth Muscle of Guinea-pig Taenia Caeci

2015 ◽  
pp. 1-19 ◽  
Author(s):  
Shigeru Hishinuma ◽  
Masanori Kurokawa ◽  
Masaatsu K. Uchida ◽  
Tomokazu Oshima
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Dual Processes ◽  
Cellular Responsiveness

scholarly journals Large-conductance voltage- and Ca2+-activated K+ channel regulation by protein kinase C in guinea pig urinary bladder smooth muscle

2014 ◽  
Vol 306 (5) ◽  
pp. C460-C470 ◽  
Author(s):  
Kiril L. Hristov ◽  
Amy C. Smith ◽  
Shankar P. Parajuli ◽  
John Malysz ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Membrane Potential ◽  
Guinea Pig ◽  
Bk Channels ◽  
Bk Channel ◽  
K Channel ◽  
Channel Activity ◽  
Open Probability ◽  
Channel Regulation ◽  
Pkc Activation

Large-conductance voltage- and Ca2+-activated K+ (BK) channels are critical regulators of detrusor smooth muscle (DSM) excitability and contractility. PKC modulates the contraction of DSM and BK channel activity in non-DSM cells; however, the cellular mechanism regulating the PKC-BK channel interaction in DSM remains unknown. We provide a novel mechanistic insight into BK channel regulation by PKC in DSM. We used patch-clamp electrophysiology, live-cell Ca2+ imaging, and functional studies of DSM contractility to elucidate BK channel regulation by PKC at cellular and tissue levels. Voltage-clamp experiments showed that pharmacological activation of PKC with PMA inhibited the spontaneous transient BK currents in native freshly isolated guinea pig DSM cells. Current-clamp recordings revealed that PMA significantly depolarized DSM membrane potential and inhibited the spontaneous transient hyperpolarizations in DSM cells. The PMA inhibitory effects on DSM membrane potential were completely abolished by the selective BK channel inhibitor paxilline. Activation of PKC with PMA did not affect the amplitude of the voltage-step-induced whole cell steady-state BK current or the single BK channel open probability (recorded in cell-attached mode) upon inhibition of all major Ca2+ sources for BK channel activation with thapsigargin, ryanodine, and nifedipine. PKC activation with PMA elevated intracellular Ca2+ levels in DSM cells and increased spontaneous phasic and nerve-evoked contractions of DSM isolated strips. Our results support the concept that PKC activation leads to a reduction of BK channel activity in DSM via a Ca2+-dependent mechanism, thus increasing DSM contractility.

Action of the Na+ ionophore monensin on vascular smooth muscle of guinea-pig aorta

1984 ◽  
Vol 100 (3-4) ◽  
pp. 299-307 ◽  
Author(s):  
H. Ozaki ◽  
T. Kishimoto ◽  
S. Chihara ◽  
H. Umeno ◽  
N. Urakawa
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Vascular Smooth Muscle ◽  
Ionophore Monensin

Some observations on the potassium-induced contracture in guinea-pig ureteric smooth muscle

1980 ◽  
Vol 149 (1) ◽  
pp. 415-426 ◽  
Author(s):  
J. M. Allen ◽  
J. B. Bridges ◽  
S. W. Crooks ◽  
H. A. Hamilton
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig

scholarly journals l-NG-nitro arginine inhibits non-adrenergic, non-cholinergic relaxations of guinea-pig isolated tracheal smooth muscle

1990 ◽  
Vol 100 (4) ◽  
pp. 663-664 ◽  
Author(s):  
John F. Tucker ◽  
Sandra R. Brave ◽  
Litsa Charalambous ◽  
Adrian J. Hobbs ◽  
Alan Gibson
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Tracheal Smooth Muscle

Sa1734 Relaxations Induced by Testosterone and Dihydrotestosterone in Isolated Guinea-Pig Gallbladder Smooth Muscle Strips

2013 ◽  
Vol 144 (5) ◽  
pp. S-294
Author(s):  
Young Don Kim ◽  
Hyun Il Seo ◽  
Jong Kyu Park ◽  
Sang Jin Lee ◽  
Koon Hee Han ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig
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