Establishment and Characterization of Human Sarcoma Lines Transplanted into Nude Mice

1996 ◽  
pp. 57-60
Author(s):  
J. Hoffmann ◽  
U. Stein ◽  
P. Schmidt-Peter ◽  
W. Hänsch ◽  
H. Naundorf ◽  
...  
Keyword(s):  
Nude Mice ◽  
Human Sarcoma

Characterization of a specific signal from human pancreatic tumors heterotransplanted into nude mice

1993 ◽  
Vol 13 (3) ◽  
Author(s):  
C. Chemin-Thomas ◽  
J. Esclassan ◽  
C. Palevody ◽  
E. Hollande
Keyword(s):  
Nude Mice ◽  
Pancreatic Tumors ◽  
Specific Signal

Generation and characterization of human monoclonal antibody HMD4 against ovarian carcinoma and the study of radioimmunoimaging in nude mice

1990 ◽  
Vol 1 (2) ◽  
pp. 104-110 ◽  
Author(s):  
Henian Qian ◽  
Heng Cui ◽  
Jie Feng ◽  
Tianyuan Fu ◽  
Ping Wei ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ovarian Carcinoma ◽  
Nude Mice ◽  
Human Monoclonal Antibody

Endocrine characterization of a human ovarian carcinoma (BG-1) established in nude mice

Steroids ◽  
1989 ◽  
Vol 54 (6) ◽  
pp. 593-606 ◽  
Author(s):  
Stephen J. Zimniski ◽  
Roberto E. Garola ◽  
Karin Fendl ◽  
C.Matthew Peterson
Keyword(s):  
Ovarian Carcinoma ◽  
Nude Mice ◽  
Human Ovarian Carcinoma

scholarly journals Hice1, a Novel Microtubule-Associated Protein Required for Maintenance of Spindle Integrity and Chromosomal Stability in Human Cells

2008 ◽  
Vol 28 (11) ◽  
pp. 3652-3662 ◽  
Author(s):  
Guikai Wu ◽  
Yi-Tzu Lin ◽  
Randy Wei ◽  
Yumay Chen ◽  
Zhiyin Shan ◽  
...  
Keyword(s):  
Cold Treatment ◽  
Coiled Coil ◽  
Spindle Pole ◽  
Terminal Region ◽  
Mitotic Spindles ◽  
Mitotic Progression ◽  
Physiological Concentration ◽  
Chromosomal Stability ◽  
Human Sarcoma

ABSTRACT Spindle integrity is critical for efficient mitotic progression and accurate chromosome segregation. Deregulation of spindles often leads to structural and functional aberrations, ultimately promoting segregation errors and aneuploidy, a hallmark of most human cancers. Here we report the characterization of a previously identified human sarcoma antigen (gene located at 19p13.11), Hice1, an evolutionarily nonconserved 46-kDa coiled-coil protein. Hice1 shows distinct cytoplasmic localization and associates with interphase centrosomes and mitotic spindles, preferentially at the spindle pole vicinity. Depletion of Hice1 by RNA interference resulted in abnormal and unstable spindle configurations, mitotic delay at prometaphase and metaphase, and elevated aneuploidy. Conversely, loss of Hice1 had minimal effects on interphase centrosome duplication. We also found that both full-length Hice1 and Hice1-N1, which is composed of 149 amino acids of the N-terminal region, but not the mutant lacking the N-terminal region, exhibited activities of microtubule bundling and stabilization at a near-physiological concentration. Consistently, overexpression of Hice1 rendered microtubule bundles in cells resistant to nocodazole- or cold-treatment-induced depolymerization. These results demonstrate that Hice1 is a novel microtubule-associated protein important for maintaining spindle integrity and chromosomal stability, in part by virtue of its ability to bind, bundle, and stabilize microtubules.

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