Five-Year Breeding Data, Immunology, and Tumor Take Rates of an NMRI Nude Mouse Colony

1996 ◽  
pp. 12-18 ◽  
Author(s):  
Thilo Spruβ ◽  
Richard Schlemmer ◽  
Günther Bernhardt ◽  
Franz Wiesenmeyer ◽  
Oskar Baumann
Keyword(s):  
Nude Mouse

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

A Nude Mouse Model as an in vivo Infectivity Assay for Cryptospomdiosis

1993 ◽  
Vol 27 (3-4) ◽  
pp. 65-68 ◽  
Author(s):  
B. H. Kwa ◽  
M. Moyad ◽  
M. A. Pentella ◽  
J. B. Rose
Keyword(s):  
Drinking Water ◽  
Mouse Model ◽  
Nude Mouse ◽  
Cryptosporidium Parvum ◽  
Nude Mice ◽  
Surface Waters ◽  
Nude Mouse Model ◽  
Infectivity Assay ◽  
Limiting Dilution

Cryptosporidium parvum is an important patliogen of diarrlieal disease which has been implicated in several outbreaks associated with contamination of surface waters. In monitoring for C. parvum in drinking water sources, it is important to asce tain the viability, and more importantly, the infectivity of low numbers of recovered oocysts. Groups of 10 Balb/C nude (nu/nu) mice, 4-8 weeks old at time of inoculation, were infected with C. parvum oocysts from naturally infected calves and purified using Sheather's sucrose gradients. Oocysts were counted using the Merifluor IFA kit (Meridian). Each group of 10 mice were infected with 1,10,100 and 1000 oocysts respectively. Numbers of oocysts per inoculation were determined by limiting dilution, and parallel inocula were counted microscopically to ascertain the accuracy of the dilutions. Two uninfected nude mice were kept in each cage to serve as controls. Mouse stools were collected every 4 days, concentrated using the Fekal Kontrate Concentration Kit (Meridian) and oocysts were counted with a UV microscope using the Merifluor IFA Kit (Meridian). Oocyst counts were expressed in terms of number of oocyst/g feces. Mice inoculated with 1000 oocysts began to shed oocysts on day 32, mice inoculated with 100 oocysts began to shed on days 44-48, mice inoculated with 10 oocysts began to shed on days 56-60, and mice inoculated with 1 oocyst shed on days 68-88. All infected mice continued to shed oocysts intermittently and with variable oocyst counts until day 180 when the experiment was terminated. This study established that it is possible to infect nude mice with very low numbers, down to a single oocyst. We are currently in the process of correlating the nude mouse assay with other viability assays.

scholarly journals Efficacy of Tumor-Targeting Salmonella A1-R on a Melanoma Patient-Derived Orthotopic Xenograft (PDOX) Nude-Mouse Model

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160882 ◽  
Author(s):  
Mako Yamamoto ◽  
Ming Zhao ◽  
Yukihiko Hiroshima ◽  
Yong Zhang ◽  
Elizabeth Shurell ◽  
...  
Keyword(s):  
Mouse Model ◽  
Nude Mouse ◽  
Melanoma Patient ◽  
Tumor Targeting ◽  
Nude Mouse Model ◽  
Orthotopic Xenograft
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