Effects of ?3 Fatty Acid Ethyl Esters on Monocyte Tissue Factor Expression

2015 ◽  
pp. 55-59 ◽  
Author(s):  
Elena Tremoli ◽  
Sonia Eligini ◽  
Susanna Colli ◽  
Paola Maderna ◽  
Franca Marangoni ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Tissue Factor ◽  
Fatty Acid Ethyl Esters ◽  
Ethyl Esters ◽  
Tissue Factor Expression ◽  
Factor Expression

The Factor VIII Bypassing Activity of Prothrombin Complex Concentrates: The Roles of Factor VIla and of Endothelial Cell Tissue Factor

1991 ◽  
Vol 66 (05) ◽  
pp. 559-564 ◽  
Author(s):  
Jerome M Teitel
Keyword(s):  
Factor Viii ◽  
Tissue Factor ◽  
Factor Viia ◽  
Factor Xa ◽  
Procoagulant Activity ◽  
Tissue Factor Expression ◽  
Prothrombin Complex Concentrates ◽  
Cell Tissue ◽  
Factor Expression ◽  
Necrosis Factor

SummaryAn experimental model incorporating cultured endothelial cells (EC) was used to study the "factor VIII bypassing" activity of prothrombin complex concentrates (PCC), a property exploited in the treatment of hemophiliacs with alloantibodies to factor VIII. Two PCC preparations were ineffective as stimuli of tissue factor expression by EC. However, incubation with a combination of PCC plus endotoxin (lipopolysaccharide, LPS) or tumor necrosis factor (TNF) induced much greater tissue factor expression than was seen in response to either substance alone. PCC expressed an additional direct procoagulant activity at the EC surface, which could not be attributed to either thrombin or factor Xa, and which was diminished by an anti-tissue factor antibody. Therefore factor VIIa, which was detectable in both PCC preparations, likely provided this additional direct procoagulant activity at the EC surface. We also excluded the possibility that coagulation proteases contained in or generated in the presence of PCC are protected from inactivation by AT III. Therefore, PCC can indirectly bypass factor VIII by enhancing induced endothelial tissue factor expression, and also possess direct procoagulant activity, probably mediated by factor VIIa.

scholarly journals Fatty acid ethyl esters in meconium and substance use in adolescence

2021 ◽  
Vol 83 ◽  
pp. 106946
Author(s):  
Meeyoung O. Min ◽  
Sonia Minnes ◽  
Hasina Momotaz ◽  
Lynn T. Singer ◽  
Anna Wasden ◽  
...  
Keyword(s):  
Substance Use ◽  
Fatty Acid ◽  
Fatty Acid Ethyl Esters ◽  
Ethyl Esters

scholarly journals Development and validation of a method for the simultaneous analysis of fatty acid ethyl esters, ethyl sulfate and ethyl glucuronide in neonatal meconium: application in two cases of alcohol consumption during pregnancy

2021 ◽  
Vol 413 (11) ◽  
pp. 3093-3105
Author(s):  
Mateusz Kacper Woźniak ◽  
Laura Banaszkiewicz ◽  
Justyna Aszyk ◽  
Marek Wiergowski ◽  
Iwona Jańczewska ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Alcohol Consumption ◽  
Solid Phase ◽  
Fatty Acid Ethyl Esters ◽  
Ethyl Esters ◽  
Ethyl Glucuronide ◽  
Published Data ◽  
Ethyl Sulfate ◽  
Analytical Approaches

AbstractAlcohol consumption during pregnancy constitutes one of the leading preventable causes of birth defects and neurodevelopmental disorders in the exposed children. Fatty acid ethyl esters (FAEEs), ethyl glucuronide (EtG) and ethyl sulfate (EtS) have been studied as potential biomarkers of alcohol consumption. However, most analytical approaches proposed for their analysis in meconium samples consist of separated extraction procedures requiring the use of two meconium aliquots, which is costly in terms of both time and materials. Therefore, the aim of this study was to develop and validate a method for the simultaneous extraction of 9 FAEEs, EtG and EtS from one meconium aliquot. The sample was homogenized using methanol, and then FAEEs were extracted with hexane while EtG and EtS were isolated using acetonitrile. Then, extracts were applied to solid-phase extraction columns and analysed by gas chromatography mass spectrometry (FAEEs) and liquid chromatography tandem mass spectrometry (EtG and EtS). Calibration curves were linear with r values greater than 0.99. The LODs ranged from 0.8 to 7.5 ng/g for FAEEs and were 0.2 ng/g and 0.8 ng/g for EtS and EtG, respectively. LOQs ranged from 5 to 25 ng/g for FAEEs and were 1 ng/g and 2.5 ng/g for EtS and EtG, respectively. Accuracies and precisions were between 93.8 and 107% and between 3.5 and 9.7%, respectively. The recovery values ranged from 89.1 to 109%. The method proved to be sensitive, specific, simple and fast and allowed for the reduction of the amount of organic solvent used for extraction compared to other published data while higher recoveries were obtained. The method was used for analysis of meconium samples in two cases of mothers who were consuming alcohol during pregnancy.

Upregulation of tissue factor expression by avastin: ex vivo and in vitro data

2012 ◽  
Vol 129 ◽  
pp. S170
Author(s):  
E. Napoleone ◽  
A. Cutrone ◽  
D. Cugino ◽  
R. Tambaro ◽  
A. De Curtis ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Ex Vivo ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Vitro Data ◽  
In Vitro Data

Nutritional compounds influence tissue factor expression and inflammation of chronic kidney disease patients in vitro

Nutrition ◽  
2011 ◽  
Vol 27 (9) ◽  
pp. 967-972 ◽  
Author(s):  
Cecilia M. Shing ◽  
Murray J. Adams ◽  
Robert G. Fassett ◽  
Jeff S. Coombes
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Tissue Factor ◽  
Tissue Factor Expression ◽  
Factor Expression ◽  
Nutritional Compounds
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