Clinical Correlations for Cell Culture Assays Based on the Concept of Total Tumor Cell Kill

1994 ◽  
pp. 82-90 ◽  
Author(s):  
Larry M. Weisenthal
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Cell Kill ◽  
Clinical Correlations

scholarly journals Theory and Experimental Validation of a Spatio-temporal Model of Chemotherapy Transport to Enhance Tumor Cell Kill

2016 ◽  
Vol 12 (6) ◽  
pp. e1004969 ◽  
Author(s):  
Zhihui Wang ◽  
Romica Kerketta ◽  
Yao-Li Chuang ◽  
Prashant Dogra ◽  
Joseph D. Butner ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Experimental Validation ◽  
Cell Kill ◽  
Temporal Model ◽  
Spatio Temporal ◽  
Enhance Tumor Cell

scholarly journals Correlation between the mixed lymphocyte tumor cell culture reaction (MLTR) and some histological characteristics in esophageal cancer.

1980 ◽  
Vol 132 (3) ◽  
pp. 267-276
Author(s):  
YOSHIKI KURIYA ◽  
TETSURO NISHIHIRA ◽  
SHOZO MORI ◽  
TOSHIO WATANABE ◽  
TAIJI SASAKI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Esophageal Cancer ◽  
Tumor Cell ◽  
Tumor Cell Culture ◽  
Histological Characteristics

scholarly journals Cultured Cell Sublines Highly Susceptible to Prion Infection

2000 ◽  
Vol 74 (9) ◽  
pp. 4377-4386 ◽  
Author(s):  
Patrick J. Bosque ◽  
Stanley B. Prusiner
Keyword(s):  
Cell Culture ◽  
Infected Cell ◽  
Tumor Cell ◽  
Cell Lines ◽  
Uninfected Cell ◽  
Resistant Cell ◽  
Cultured Cell ◽  
Prion Infection ◽  
Blot Technique ◽  
Prion Propagation

ABSTRACT Cultured cell lines infected with prions produce an abnormal isoform of the prion protein (PrPSc). In order to derive cell lines producing sufficient quantities of PrPSc for most studies, it has been necessary to subclone infected cultures and select the subclones producing the largest amounts of PrPSc. Since postinfection cloning can introduce differences between infected and uninfected cell lines, we sought an approach to generate prion-infected cell lines that would avoid clonal artifacts. Using an improved cell blot technique, which permits sensitive and rapid comparison of PrPSc levels in multiple independent cell cultures, we discovered marked heterogeneity with regard to prion susceptibility in tumor cell sublines. We exploited this heterogeneity to derive sublines which are highly susceptible to prion infection and used these cells to generate prion-infected lines without further subcloning. These infected sublines can be compared to the cognate uninfected cultures without interference from cloning artifacts. We also used susceptible cell lines and our modified cell blot procedure to develop a sensitive and reproducible quantitative cell culture bioassay for prions. We found that the sublines were at least 100-fold more susceptible to strain RML prions than to strain ME7 prions. Comparisons between scrapie-susceptible and -resistant cell lines may reveal factors that modulate prion propagation.

Comparison between clinical response and in vitro drug sensitivity of primary human tumors in the adhesive tumor cell culture system.

1987 ◽  
Vol 5 (12) ◽  
pp. 1912-1921 ◽  
Author(s):  
J A Ajani ◽  
F L Baker ◽  
G Spitzer ◽  
A Kelly ◽  
W Brock ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Culture System ◽  
Distinct Advantage ◽  
Cell Culture System ◽  
True Value ◽  
Tumor Cell Culture ◽  
Prospective Trials ◽  
Tumor Types

The newly described adhesive tumor cell culture system (ATCCS) offers a distinct advantage over other assays in that it has a high plating efficiency requiring low cell inoculum, it affords workable assays in approximately 70% of specimens from the heterogenous tumor types, and it has the ability to assay up to nine drugs at four different concentrations. Clinical correlations based on the ATCCS were obtained in 65 patients undergoing 71 clinical trials. Patients with melanoma, lung cancer, and sarcoma dominated the group. The most active in vitro drug was correlated per clinical trial. Thirteen of 17 (76%) sensitive in vitro predictions and 51 of 54 (94%) resistant in vitro predictions were accurate. The assay in this study had a sensitivity of 81% and specificity of 93%. These preliminary results are encouraging and warrant prospective trials to establish the true value of this assay to patients.

scholarly journals Senescence Process in Primary Wilms' Tumor Cell Culture Induced by p53 Independent p21 Expression

2016 ◽  
Vol 7 (13) ◽  
pp. 1867-1876 ◽  
Author(s):  
Korkiat Theerakitthanakul ◽  
Jirakrit Saetang ◽  
Jirasak Kruatong ◽  
Potchanapond Graidist ◽  
Pritsana Raungrut ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Wilms Tumor ◽  
Tumor Cell Culture

Dynamics of T-cell infiltration during the course of ovarian cancer: The gradual shift from a Th17 effector cell response to a predominant infiltration by regulatory T cells.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e22129-e22129
Author(s):  
Simona Partlova ◽  
Anna Fialova ◽  
Ludek Sojka ◽  
Lukas Rob ◽  
Jirina Bartunkova ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Culture ◽  
Flow Cytometry ◽  
T Cells ◽  
Regulatory T Cells ◽  
Tumor Cell ◽  
Immune Cells ◽  
Tumor Tissue ◽  
Tumor Cell Culture ◽  
Culture Supernatants

e22129 Background: Ovarian cancer is diagnosed in more than 190,000 new patients every year and is known to have the highest mortality rate among gynaecologic cancers. The type of immune cells that are present within the tumor microenvironment can play a crucial role in the survival of patients. However, little is known about the dynamics of the tumor-infiltrating immune cells during disease progression. Methods: We studied the immune cells infiltrating the tumor tissue of ovarian cancer patients at different stages of disease. We analysed the patterns of T lymphocytes in fresh tumor tissue as well as blood samples of 44 newly diagnosed ovarian cancer patients by flow cytometry. To evaluate whether regulatory T cells (Tregs) develop in situ or migrate to tumor tissue, we measured a concentration of chemokine CCL22 in tumor cell culture supernatants. We also determined the expression of CCR4 on circulating as well as tumor-infiltrating Tregs by flow cytometry. Results: The early stages of development of ovarian carcinomas were characterized by a strong Th17 immune response, whereas in stage II patients, recruitment of high numbers of Th1 cells was observed. In disseminated tumors (stage III-IV), we detected a dominant population of Helios+ activated regulatory T cells along with high numbers of macrophages and immature myeloid dendritic cells. Tumor-infiltrating Tregs had markedly lower expression of CCR4 than circulating Tregs, and the numbers of tumor-infiltrating Tregs significantly correlated with the levels of CCL22 in ovarian tumor cell culture supernatants, suggesting their recruitment via a CCR4/CCL22 interaction. CCL22 was mainly produced by tumor cells, macrophages and mDCs in the primary ovarian tumors, and its expression markedly increased in response to IFNgamma. Conclusions: Taken together, the specific recruitment of Tregs, probably triggered by inflammatory stimuli, leads to a significant immune suppression in the advanced stages of ovarian cancer.

scholarly journals Tumor cell culture survival following glucose and glutamine deprivation at typical physiological concentrations

Nutrition ◽  
2014 ◽  
Vol 30 (2) ◽  
pp. 218-227 ◽  
Author(s):  
Edward Henry Mathews ◽  
B. André Stander ◽  
Annie M. Joubert ◽  
Leon Liebenberg
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Glutamine Deprivation ◽  
Tumor Cell Culture

Facile preparation of a novel mulberry silk fibroin scaffold for three-dimensional tumor cell culture

2015 ◽  
Vol 143 ◽  
pp. 8-11 ◽  
Author(s):  
Kai Xin ◽  
Puyuan Wu ◽  
Rutian Li ◽  
Jia Wei ◽  
Huizi Sha ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Silk Fibroin Scaffold ◽  
Tumor Cell Culture ◽  
Facile Preparation
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