Assessment of Liver Microcirculation with the Multiple Indicator Dilution Technique

2015 ◽  
pp. 40-51 ◽  
Author(s):  
J. Reichen
Keyword(s):  
Indicator Dilution ◽  
Dilution Technique ◽  
Liver Microcirculation ◽  
Multiple Indicator Dilution ◽  
Indicator Dilution Technique ◽  
Multiple Indicator

The multiple-indicator dilution technique for characterization of normal and retrograde flow in once-through rat liver perfusions

Hepatology ◽  
1989 ◽  
Vol 9 (2) ◽  
pp. 285-296 ◽  
Author(s):  
Marie V. St-Pierre ◽  
Andreas J. Schwab ◽  
Carl A. Goresky ◽  
Wai-Fong Lee ◽  
K. Sandy Pang
Keyword(s):  
Rat Liver ◽  
Indicator Dilution ◽  
Retrograde Flow ◽  
Dilution Technique ◽  
Multiple Indicator Dilution ◽  
Indicator Dilution Technique ◽  
Multiple Indicator

Characterization of calcium deprivation-induced cholestasis in the perfused rat liver

1985 ◽  
Vol 249 (1) ◽  
pp. G48-G57 ◽  
Author(s):  
J. Reichen ◽  
F. Berr ◽  
M. Le ◽  
G. H. Warren
Keyword(s):  
Rat Liver ◽  
Bile Flow ◽  
Tritiated Water ◽  
Isolated Hepatocytes ◽  
Indicator Dilution ◽  
Perfused Rat Liver ◽  
Dilution Technique ◽  
Multiple Indicator Dilution ◽  
Indicator Dilution Technique ◽  
Multiple Indicator

To elucidate the role of calcium in regulation of canalicular bile flow, we studied biliary sucrose permeability and the transport kinetics of taurocholate in the in situ perfused rat liver. Calcium deprivation did not adversely affect viability or ultrastructural appearances of the liver. Removal of calcium led to initial choleresis followed by cholestasis dependent on external ionized calcium concentration. Biliary recovery of [14C]sucrose relative to that of tritiated water was determined by a biliary multiple-indicator dilution technique. Analysis in terms of irreversible thermodynamics suggested that biliary permeability to sucrose increased due to a change in the sieving coefficient from 0.135 to 0.435. Biliary recovery of taurocholate was significantly (P less than 0.001) reduced in low-calcium medium (from 79.6 +/- 6.5 to 17.6 +/- 11.8%). This was not due to a defect in hepatocellular uptake of taurocholate as determined in the perfused rat liver by a multiple-indicator dilution technique and in isolated hepatocytes. We conclude that calcium deprivation-induced cholestasis is characterized by an increased biliary permeability, a defect in cellular translocation and/or canalicular secretion of bile salts, and a defect in bile salt-independent bile flow.

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