Combined In Vitro/In Vivo Test Procedure with Human Tumor Xenografts for New Drug Development

1992 ◽  
pp. 321-351 ◽  
Author(s):  
H. H. Fiebig ◽  
D. P. Berger ◽  
W. A. Dengler ◽  
E. Wallbrecher ◽  
B. R. Winterhalter
Keyword(s):  
Drug Development ◽  
Test Procedure ◽  
Human Tumor ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts ◽  
In Vivo Test ◽  
New Drug ◽  
New Drug Development

In vitro and in vivo anticancer activity of mitozolomide and sparsomycin in human tumor xenografts, murine tumors and human bone marrow

1990 ◽  
Vol 116 (6) ◽  
pp. 550-556 ◽  
Author(s):  
Heinz H. Fiebig ◽  
Dietmar P. Berger ◽  
Karin Köpping ◽  
Harry C. J. Ottenheijm ◽  
Zbigniew Zylicz
Keyword(s):  
Bone Marrow ◽  
Anticancer Activity ◽  
Human Tumor ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts ◽  
Murine Tumors

In vitro and in vivo evaluation of US-NCI compounds in human tumor xenografts

1990 ◽  
Vol 17 (2-3) ◽  
pp. 109-117 ◽  
Author(s):  
Heinz-Herbert Fiebig ◽  
Dietmar P. Berger ◽  
Bernd R. Winterhalter ◽  
Jacqueline Plowman
Keyword(s):  
Human Tumor ◽  
In Vivo Evaluation ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts

scholarly journals A Literature Review of the Physicochemical, Physiological and Pharmaceutical Considerations in GIT Absorption of Drugs.

2017 ◽  
Vol II (I) ◽  
pp. 18-33
Author(s):  
Rabia Mazhar ◽  
Fariah Qaise ◽  
Sana Ali Zahra ◽  
Syeda Komal Fatima ◽  
Imran Khan
Keyword(s):  
Drug Development ◽  
Drug Absorption ◽  
Oral Route ◽  
Physiological Factors ◽  
New Drug ◽  
Physico Chemical ◽  
Key Factor ◽  
New Drug Development

Oral route of drug administration is the most common among all routes and hence their pharmacokinetic and pharmacodynamic factors are substantial to study. Among such factors, GIT absorption is the key factor in new drug development affecting the efficacy as well as safety of the drug. Different protocols have now been developed for the usage of in vitro also the in vivo as well as in situ methods in drug absorption determination. In this article, different mechanisms like passive diffusion, pore transport, ionic-mediated transport, endocytosis and other mechanisms involved in drug absorption will be explained. Moreover, different factors i.e. physico-chemical, pharmaceutical and physiological factors that affect drug absorption have been summarized as they play a significant role in the research studies for new drug development. Different absorption determining methods are also discussed.

Monoclonal Antibodies to Six-Transmembrane Epithelial Antigen of the Prostate-1 Inhibit Intercellular Communication In vitro and Growth of Human Tumor Xenografts In vivo

2007 ◽  
Vol 67 (12) ◽  
pp. 5798-5805 ◽  
Author(s):  
Pia M. Challita-Eid ◽  
Kendall Morrison ◽  
Soudabeh Etessami ◽  
Zili An ◽  
Karen J. Morrison ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Intercellular Communication ◽  
Human Tumor ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts

Prevascularized, Co-Culture Model for Breast Cancer Drug Development

2012 ◽  
Author(s):  
Lauren Marshall ◽  
Isabel Löwstedt ◽  
Paul Gatenholm ◽  
Joel Berry
Keyword(s):  
Breast Cancer ◽  
Drug Development ◽  
Three Dimensional ◽  
Human Tumor ◽  
3D Models ◽  
Cancer Drug ◽  
Cancer Therapies ◽  
Anti Cancer

The objective of this study was to create 3D engineered tissue models to accelerate identification of safe and efficacious breast cancer drug therapies. It is expected that this platform will dramatically reduce the time and costs associated with development and regulatory approval of anti-cancer therapies, currently a multi-billion dollar endeavor [1]. Existing two-dimensional (2D) in vitro and in vivo animal studies required for identification of effective cancer therapies account for much of the high costs of anti-cancer medications and health insurance premiums borne by patients, many of whom cannot afford it. An emerging paradigm in pharmaceutical drug development is the use of three-dimensional (3D) cell/biomaterial models that will accurately screen novel therapeutic compounds, repurpose existing compounds and terminate ineffective ones. In particular, identification of effective chemotherapies for breast cancer are anticipated to occur more quickly in 3D in vitro models than 2D in vitro environments and in vivo animal models, neither of which accurately mimic natural human tumor environments [2]. Moreover, these 3D models can be multi-cellular and designed with extracellular matrix (ECM) function and mechanical properties similar to that of natural in vivo cancer environments [3].

Nude mice with lacZ transduced human tumor xenografts as an in vivo model for invasion and metastasis

Lung Cancer ◽  
1991 ◽  
Vol 7 ◽  
pp. 30 ◽  
Author(s):  
Mogens Spang-Thomsen ◽  
James A. Zwiebel ◽  
Jørgen Rygaard ◽  
Nils Brünner
Keyword(s):  
Nude Mice ◽  
Human Tumor ◽  
In Vivo Model ◽  
Invasion And Metastasis ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts

scholarly journals Evaluation of 3-l- and 3-d-[18F]Fluorophenylalanines as PET Tracers for Tumor Imaging

Cancers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 6030
Author(s):  
Felicia Krämer ◽  
Benedikt Gröner ◽  
Chris Hoffmann ◽  
Austin Craig ◽  
Melanie Brugger ◽  
...  
Keyword(s):  
Amino Acid ◽  
Tumor Imaging ◽  
Human Tumor ◽  
Transport Systems ◽  
Acid Decarboxylase ◽  
Tumor Xenografts ◽  
Tumor Delineation ◽  
Mcf 7

Purpose: The preclinical evaluation of 3-l- and 3-d-[18F]FPhe in comparison to [18F]FET, an established tracer for tumor imaging. Methods: In vitro studies were conducted with MCF-7, PC-3, and U87 MG human tumor cell lines. In vivo µPET studies were conducted in healthy rats with/without the inhibition of peripheral aromatic l-amino acid decarboxylase by benserazide pretreatment (n = 3 each), in mice bearing subcutaneous MCF-7 or PC-3 tumor xenografts (n = 10), and in rats bearing orthotopic U87 MG tumor xenografts (n = 14). Tracer accumulation was quantified by SUVmax, SUVmean and tumor-to-brain ratios (TBrR). Results: The uptake of 3-l-[18F]FPhe in MCF-7 and PC-3 cells was significantly higher relative to [18F]FET. The uptake of all three tracers was significantly reduced by the suppression of amino acid transport systems L or ASC. 3-l-[18F]FPhe but not 3-d-[18F]FPhe exhibited protein incorporation. In benserazide-treated healthy rats, brain uptake after 42–120 min was significantly higher for 3-d-[18F]FPhe vs. 3-l-[18F]FPhe. [18F]FET showed significantly higher uptake into subcutaneous MCF-7 tumors (52–60 min p.i.), while early uptake into orthotopic U87 MG tumors was significantly higher for 3-l-[18F]FPhe (SUVmax: 3-l-[18F]FPhe, 107.6 ± 11.3; 3-d-[18F]FPhe, 86.0 ± 4.3; [18F]FET, 90.2 ± 7.7). Increased tumoral expression of LAT1 and ASCT2 was confirmed immunohistologically. Conclusion: Both novel tracers enable accurate tumor delineation with an imaging quality comparable to [18F]FET.

MRI of human tumor xenografts in vivo: Proton relaxation times and extracellular tumor volume

1995 ◽  
Vol 13 (5) ◽  
pp. 693-700 ◽  
Author(s):  
Ingvil Jakobsen ◽  
Heidi Lyng ◽  
Olav Kaalhus ◽  
Einar K. Rofstad
Keyword(s):  
Tumor Volume ◽  
Relaxation Times ◽  
Human Tumor ◽  
Proton Relaxation ◽  
Human Tumor Xenografts ◽  
Tumor Xenografts
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