Lipid-Associated Plasma Membrane Properties of White Blood Cells1

2015 ◽  
pp. 61-71
Author(s):  
C. H�bner ◽  
B. Finckh ◽  
E. Fl�gel ◽  
K. Fock ◽  
H. A. Lehr ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Properties

scholarly journals Phenothiazines alter plasma membrane properties and sensitize cancer cells to injury by inhibiting annexin-mediated repair

2021 ◽  
pp. 101012
Author(s):  
Anne Sofie Busk Heitmann ◽  
Ali Asghar Hakami Zanjani ◽  
Martin Berg Klenow ◽  
Anna Mularski ◽  
Stine Lauritzen Sønder ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cancer Cells ◽  
Membrane Properties

Effects of ZnCl2 on ROS generation, plasma membrane properties, and changes in protein expression in grapevine root explants

Biologia ◽  
2016 ◽  
Vol 71 (5) ◽  
Author(s):  
Vladimír Repka ◽  
Roderik Fiala ◽  
Milada Čiamporová ◽  
Ján Pavlovkin
Keyword(s):  
Plasma Membrane ◽  
Protein Expression ◽  
Adventitious Root ◽  
Membrane Properties ◽  
Ros Generation ◽  
Root Explants

AbstractThis study is aimed at the responses of grapevine adventitious root explants to zinc (Zn

scholarly journals Methylprednisolone inhibits uptake of Ca2+ and Na+ ions into concanavalin A-stimulated thymocytes

1997 ◽  
Vol 326 (2) ◽  
pp. 329-332 ◽  
Author(s):  
Frank BUTTGEREIT ◽  
Stefan KRAUSS ◽  
Martin D. BRAND
Keyword(s):  
Plasma Membrane ◽  
Concanavalin A ◽  
Membrane Properties ◽  
Mitochondrial Electron Transport Chain ◽  
Oxidation Reactions ◽  
Transport Chain ◽  
Cord Injury ◽  
Na Ions ◽  
Atp Supply ◽  
Na Uptake

The glucocorticoid drug methylprednisolone inhibits respiration in concanavalin A-stimulated rat thymocytes at concentrations that are relevant to its acute clinical efficacy against autoimmune diseases and spinal cord injury. Methylprednisolone affects several processes, including ion cycling, substrate oxidation reactions and RNA/DNA synthesis. The inhibition of respiration used to drive ATP-consuming cycles of Ca2+ and Na+ ions across the plasma membrane has been proposed to be either primary or secondary to restriction of cellular ATP supply. By comparing the effects of methylprednisolone with those of myxothiazol, an inhibitor of the mitochondrial electron transport chain, we show that the effects of methylprednisolone on Ca2+ and Na+ cycling are primary. We propose that methylprednisolone acts by affecting membrane properties to inhibit Ca2+ and Na+ uptake across the plasma membrane and to increase H+ uptake across the mitochondrial membrane, and that other effects are secondary.

Effect of chitosan on growth and plasma membrane properties of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill

2010 ◽  
Vol 97 (3) ◽  
pp. 275-278 ◽  
Author(s):  
J. García-Rincón ◽  
J. Vega-Pérez ◽  
M.G. Guerra-Sánchez ◽  
A.N. Hernández-Lauzardo ◽  
A. Peña-Díaz ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Properties ◽  
Rhizopus Stolonifer

scholarly journals Exploration of Lipid Metabolism in Relation with Plasma Membrane Properties of Duchenne Muscular Dystrophy Cells: Influence of L-Carnitine

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49346 ◽  
Author(s):  
Françoise Le Borgne ◽  
Stéphane Guyot ◽  
Morgan Logerot ◽  
Laurent Beney ◽  
Patrick Gervais ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Metabolism ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Membrane Properties

scholarly journals Alkyl gallates display elicitor activities in tobacco plants

2017 ◽  
Author(s):  
Pascale Goupil ◽  
Razik Benouaret ◽  
Claire Richard
Keyword(s):  
Cell Death ◽  
Plasma Membrane ◽  
Target Genes ◽  
Crop Protection ◽  
Membrane Properties ◽  
List Type ◽  
Ethyl Gallate ◽  
Tobacco Plants ◽  
Tobacco Leaves ◽  
Alkyl Gallates

AbstractAlkyl gallates showed elicitor activities on tobacco in both whole plants and cell suspensions. Methyl gallate (MG), ethyl gallate (EG) and n-propyl gallate (PG) infiltration into tobacco leaves induced hypersensitive reaction-like lesions and topical production of autofluorescent compounds revealed under UV light. When sprayed on tobacco plants at 5 mM, EG promoted upregulation of defence-related genes such as the antimicrobial PR1, β-1,3-glucanase PR2, chitinase PR3 and osmotin PR5 target genes. Tobacco BY-2 cells challenged with EG underwent cell death in 48 h, significantly reduced in the presence of the protease inhibitor aprotinin. The three alkyl gallates all caused alkalinisation of the BY-2 extracellular medium, whereas gallic acid did not trigger any pH variation. Using EGTA or LaCl3, we showed that Ca2+ mobilisation occurred in BY-2 cells elicited with EG. Overall, our findings are the first evidence of alkyl gallate elicitor properties with early perception events on plasma membrane, potential hypersensitive reactions and PR-related downstream defence responses in tobacco.Highlights–Alkyl gallates elicited defence reactions in tobacco–Alkyl gallates induced local biochemical changes in tobacco leaves–Alkyl gallates caused modification of plasma membrane properties–Ethyl gallate led to defence transcript accumulation and dose-dependent cell death associated with hypersensitive response–Alkyl gallates are novel elicitor agents well-suited to crop protection schemes.

Effects of Proteus mirabilis Lipopolysaccharides with Different O-Polysaccharide Structures on the Plasma Membrane of Human Erythrocytes

2008 ◽  
Vol 63 (5-6) ◽  
pp. 460-468 ◽  
Author(s):  
Michał Arabski ◽  
Krzysztof Gwoździński ◽  
Beata Sudak ◽  
Wiesław Kaca
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Erythrocyte Membrane ◽  
Osmotic Fragility ◽  
Membrane Lipid ◽  
Membrane Properties ◽  
Physical Parameters ◽  
Chemical Structures ◽  
Lipid Fluidity ◽  
Membrane Lipid Fluidity

The effects of O33 and O49 P. mirabilis lipopolysaccharides (LPSs) on human erythrocyte membrane properties were examined. Physical parameters of the plasma membrane, such as membrane lipid fluidity, physical state of membrane proteins, and osmotic fragility, were determined. The fluidity of the lipids was estimated using three spin-labeled stearic acids of doxyl derivatives: 5-doxylstearic acid, 12-doxylstearic acid, and 16-doxylstearic acid. All the applied labels locate to different depths of the lipid layer and provide information on the ordering of phospholipid fatty acyl chain mobility. LPSs O49 increased the membrane lipid fluidity in the polar region of the lipid bilayer as indicated by spin-labeled 5-doxylstearic acid. An increase in fluidity was also observed in the deeper region using 12-doxylstearic acid only for O33 LPSs. The highest concentration of O33 LPSs (1 mg/ml) increased the motion of membrane proteins detected by the spin-label residue of iodoacetamide. These results showed different actions of O33 and O49 LPSs on the plasma membrane due to the different chemical structures of O-polysaccharides. P. mirabilis O33 and O49 LPSs did not induce changes in the membrane cytoskeleton, osmotic fragility and lipid peroxidation of erythrocytes. On the other hand a rise in the content of carbonyl compounds was observed for the highest concentrations of O33 LPS. This result indicated protein oxidation in the erythrocyte membrane. Lipid A, the hydrophobic part of LPS, did not change the membrane lipid fluidity and osmotic fragility of erythrocytes. Smooth and rough forms of P. mirabilis LPSs were tested for their abilities for complement-mediated immunohemolysis of erythrocytes. Only one out of seven LPSs used was a potent agent of complement-mediated hemolysis. It was rough, Ra-type of P. mirabilis R110 LPS. The O-polysaccharide-dependent scheme of reaction is presented.

Proton conductance of the plasma membrane: properties, regulation, and functional role

1993 ◽  
Vol 265 (1) ◽  
pp. C3-C14 ◽  
Author(s):  
G. L. Lukacs ◽  
A. Kapus ◽  
A. Nanda ◽  
R. Romanek ◽  
S. Grinstein
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Second Messengers ◽  
Cell Types ◽  
Membrane Properties ◽  
Transport Systems ◽  
Extracellular Acidification ◽  
Phagocyte Nadph Oxidase ◽  
Selective Permeability ◽  
Conductive Pathway

H+ conductive pathways have been detected in the plasma membranes of a variety of cell types. The large exquisitely H(+)-selective permeability of the conductive pathway can support sizable net H+ fluxes. Although subtle differences exist among tissues and species, certain common features suggest that related transport systems are involved in all cases. The H+ conductance is gated by depolarizing voltages and is promoted by intracellular acidification. Conversely, extracellular acidification inhibits the conductance. These features facilitate net H+ efflux, while precluding potentially deleterious H+ uptake. In some cell types, activation of the conductance is additionally controlled by physiological ligands and by second messengers. The conductance most likely functions in the regulation of intracellular pH, contributing to the extrusion of H+ during repetitive depolarization of the plasma membrane, as occurs in neurons and muscle cells. This pathway may be particularly relevant in the case of phagocytes. When stimulated, these cells undergo a sustained depolarization, while generating large amounts of metabolic acid. In addition, conductive H+ fluxes may also provide counterions to neutralize the activity of electrogenic enzymes, as suggested for the phagocyte NADPH oxidase.

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