Cultivation of Human Sebocytes in vitro: Cell Characterization and Influence of Synthetic Retinoids

2015 ◽  
pp. 254-273 ◽  
Author(s):  
Christos C. Zouboulis ◽  
Longqing Xia ◽  
Bernhard Korge ◽  
Harald Gollnick ◽  
Constantin E. Orfanos
Keyword(s):  
Cell Characterization

scholarly journals Alternative Induced Pluripotent Stem Cell Characterization Criteria for In Vitro Applications

2009 ◽  
Vol 4 (3) ◽  
pp. 198-199 ◽  
Author(s):  
James Ellis ◽  
Benoit G. Bruneau ◽  
Gordon Keller ◽  
Ihor R. Lemischka ◽  
Andras Nagy ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Characterization ◽  
Induced Pluripotent

Phage display as a tool for rapid in vitro cell characterization by fluorescence imaging and Raman spectroscopy

2014 ◽  
Vol 31 ◽  
pp. S107 ◽  
Author(s):  
Laura De Plano ◽  
Federica Calabrese ◽  
Germana Lentini ◽  
Marco Nicolò ◽  
Domenico Franco ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Phage Display ◽  
Fluorescence Imaging ◽  
Cell Characterization

scholarly journals Single‐cell characterization and metabolic profiling of in vitro cultured human skeletal progenitors with enhanced in vivo bone forming capacity

2019 ◽  
Vol 9 (3) ◽  
pp. 389-402 ◽  
Author(s):  
Johanna Bolander ◽  
Tim Herpelinck ◽  
Malay Chaklader ◽  
Charikleia Gklava ◽  
Liesbet Geris ◽  
...  
Keyword(s):  
Single Cell ◽  
Metabolic Profiling ◽  
Cell Characterization

scholarly journals Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7

2015 ◽  
Vol 6 ◽  
Author(s):  
Johanna Tauriainen ◽  
Karin Gustafsson ◽  
Mårten Göthlin ◽  
Jens Gertow ◽  
Marcus Buggert ◽  
...  
Keyword(s):  
T Cells ◽  
Single Cell ◽  
Cell Characterization ◽  
Interaction Dynamics

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

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