Preliminary Characterization of a Panel of Monoclonal Antibodies to Human Factor VII

2015 ◽  
pp. 187-193
Author(s):  
R. Coppola ◽  
S. Tombesi ◽  
R. Belotti ◽  
S. Alborali ◽  
M. Gobbi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Factor ◽  
Factor Vii ◽  
Preliminary Characterization

Preliminary characterization of a panel of monoclonal antibodies to human factor VII

1991 ◽  
Vol 61 ◽  
pp. 115
Author(s):  
R. Coppola ◽  
S. Tombesi ◽  
R. Belotti ◽  
S. Alborali ◽  
M. Gobbi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Factor ◽  
Factor Vii ◽  
Preliminary Characterization

Characterization of Monoclonal Anti-human Factor VII Antibody (B101/B1) that Recognized Three-dimensional Structures near Arg at Position 79 in the First EGF-like Domain

1998 ◽  
Vol 79 (01) ◽  
pp. 104-109 ◽  
Author(s):  
Osamu Takamiya
Keyword(s):  
Monoclonal Antibodies ◽  
Tissue Factor ◽  
Human Factor ◽  
Solid Phase ◽  
Three Dimensional ◽  
Coagulation Factors ◽  
Factor Vii ◽  
Dimensional Structure ◽  
Epidermal Growth

SummaryMurine monoclonal antibodies (designated hVII-B101/B1, hVIIDC2/D4 and hVII-DC6/3D8) directed against human factor VII (FVII) were prepared and characterized, with more extensive characterization of hVII-B101/B1 that did not bind reduced FVIIa. The immunoglobulin of the three monoclonal antibodies consisted of IgG1. These antibodies did not inhibit procoagulant activities of other vitamin K-dependent coagulation factors except FVII and did not cross-react with proteins in the immunoblotting test. hVII-DC2/D4 recognized the light chain after reduction of FVIIa with 2-mercaptoethanol, and hVIIDC6/3D8 the heavy chain. hVII-B101/B1 bound FVII without Ca2+, and possessed stronger affinity for FVII in the presence of Ca2+. The Kd for hVII-B101/B1 to FVII was 1.75 x 10–10 M in the presence of 5 mM CaCl2. The antibody inhibited the binding of FVII to tissue factor in the presence of Ca2+. hVII-B101/B1 also inhibited the activation of FX by the complex of FVIIa and tissue factor in the presence of Ca2+. Furthermore, immunoblotting revealed that hVII-B101/B1 reacted with non-reduced γ-carboxyglutaminic acid (Gla)-domainless-FVII and/or FVIIa. hVII-B101/B1 showed a similar pattern to that of non-reduced proteolytic fragments of FVII by trypsin with hVII-DC2/D4 on immunoblotting test. hVII-B101/B1 reacted differently with the FVII from the dysfunctional FVII variant, FVII Shinjo, which has a substitution of Gln for Arg at residue 79 in the first epidermal growth factor (1st EGF)-like domain (Takamiya O, et al. Haemosta 25, 89-97,1995) compared with normal FVII, when used as a solid phase-antibody for ELISA by the sandwich method. hVII-B101/B1 did not react with a series of short peptide sequences near position 79 in the first EGF-like domain on the solid-phase support for epitope scanning. These results suggested that the specific epitope of the antibody, hVII-B101/B1, was located in the three-dimensional structure near position 79 in the first EGF-like domain of human FVII.

Preparation and preliminary characterization of new monoclonal antibodies versus estradiol receptor

1991 ◽  
Vol 27 ◽  
pp. S71
Author(s):  
C. Abbondanza ◽  
A. de Falco ◽  
V. Nigro ◽  
B. Moncharmont ◽  
N. Medici ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Preliminary Characterization ◽  
Estradiol Receptor

Preparation and Preliminary Characterization of Rabbit Monoclonal Antibodies Against Human Midkine

Hybridoma ◽  
2011 ◽  
Vol 30 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Xing Yao ◽  
Fu-Chu Qian ◽  
Li-Cheng Dai ◽  
Li-Shan Min
Keyword(s):  
Monoclonal Antibodies ◽  
Preliminary Characterization

scholarly journals Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus.

1983 ◽  
Vol 47 (2) ◽  
pp. 267-275 ◽  
Author(s):  
H B Greenberg ◽  
J Valdesuso ◽  
K van Wyke ◽  
K Midthun ◽  
M Walsh ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Surface Proteins ◽  
Preliminary Characterization

scholarly journals Production and Preliminary Characterization of Monoclonal Antibodies against Cationic Peanut Peroxidase

1987 ◽  
Vol 85 (1) ◽  
pp. 299-303 ◽  
Author(s):  
Chunfang Hu ◽  
Daniela Carbonera ◽  
Robert van Huystee
Keyword(s):  
Monoclonal Antibodies ◽  
Preliminary Characterization ◽  
Peanut Peroxidase

Production and preliminary characterization of monoclonal antibodies highly specific to pork fat protein

Food Control ◽  
2017 ◽  
Vol 79 ◽  
pp. 80-86 ◽  
Author(s):  
Jeong-Sook Kim ◽  
In Jun Kwon ◽  
Min-Gon Kim ◽  
Ji Yoon Chang ◽  
Won-Bo Shim
Keyword(s):  
Monoclonal Antibodies ◽  
Preliminary Characterization ◽  
Pork Fat
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