Structure-Function Analysis of the Human Transferrin Receptor: Effects of Anti-Receptor Monoclonal Antibodies on Tumor Growth1

2015 ◽  
pp. 139-147 ◽  
Author(s):  
Ian S. Trowbridge ◽  
James Collawn ◽  
Shuqian Jing ◽  
Suhaila White ◽  
Victor Esekogwu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Structure Function ◽  
Transferrin Receptor ◽  
Function Analysis ◽  
Human Transferrin ◽  
Human Transferrin Receptor

scholarly journals Do monoclonal antibodies Tü15 and Tü67 detect heterogeneity of human transferrin receptor molecules?

FEBS Letters ◽  
1984 ◽  
Vol 175 (2) ◽  
pp. 279-283 ◽  
Author(s):  
Barbara Uchańska-Ziegler ◽  
Peter Wernet ◽  
Shi Liangru ◽  
Andreas Ziegler
Keyword(s):  
Monoclonal Antibodies ◽  
Transferrin Receptor ◽  
Human Transferrin ◽  
Human Transferrin Receptor

Monoclonal Antibodies to a Purified Human Transferrin Receptor

1984 ◽  
Vol 20 (5) ◽  
pp. 441-447 ◽  
Author(s):  
B. NIKINMAA ◽  
C. A. ENNS ◽  
S. E. TONIK ◽  
H. H. SUSSMAN ◽  
J. SCHRODER
Keyword(s):  
Monoclonal Antibodies ◽  
Transferrin Receptor ◽  
Human Transferrin ◽  
Human Transferrin Receptor

Structural, functional, and tissue distribution analysis of human transferrin receptor-2 by murine monoclonal antibodies and a polyclonal antiserum

Blood ◽  
2002 ◽  
Vol 100 (10) ◽  
pp. 3782-3789 ◽  
Author(s):  
Silvia Deaglio ◽  
Andrea Capobianco ◽  
Angelita Calı̀ ◽  
Francesca Bellora ◽  
Federica Alberti ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tissue Distribution ◽  
Transferrin Receptor ◽  
Polyclonal Antiserum ◽  
Target Cells ◽  
Distribution Analysis ◽  
Human Transferrin ◽  
Normal Tissues ◽  
Human Transferrin Receptor ◽  
Transferrin Receptor 2

Human transferrin receptor-2 (TFR-2) is a protein highly homologous to TFR-1/CD71 and is endowed with the ability to bind transferrin (TF) with low affinity. High levels of TFR-2 mRNA were found in the liver and in erythroid precursors. Mutations affecting the TFR-2gene led to hemochromatosis type 3, a form of inherited iron overload. Several issues on distribution and function of the receptor were answered by raising a panel of 9 monoclonal antibodies specific for TFR-2 by immunizing mice with murine fibroblasts transfected with the human TFR-2 cDNA. A polyclonal antiserum was also produced in mice immunized with 3 peptides derived from the TFR-2 sequence, exploiting an innovative technique. The specificity of all the reagents produced was confirmed by reactivity with TFR-2+ target cells and simultaneous negativity with TFR-1+ cells. Western blot analyses showed a dominant chain of approximately 90 kDa in TFR-2 transfectants and HepG2 cell line. Analysis of distribution in normal tissues and in representative cell lines revealed that TFR-2 displays a restricted expression pattern—it is present at high levels in hepatocytes and in the epithelial cells of the small intestine, including the duodenal crypts. Exposure of human TFR-2+cells to TF-bound iron is followed by a significant up-regulation and relocalization of membrane TFR-2. The tissue distribution pattern, the behavior following exposure to iron-loaded TF, and the features of the disease resulting from TFR-2 inactivation support the hypothesis that TFR-2 contributes to body iron sensing.

scholarly journals Structural model of phospholipid-reconstituted human transferrin receptor derived by electron microscopy

Structure ◽  
1998 ◽  
Vol 6 (10) ◽  
pp. 1235-1243 ◽  
Author(s):  
Hendrik Fuchs ◽  
Uwe Lücken ◽  
Rudolf Tauber ◽  
Andreas Engel ◽  
Reinhard Geßner
Keyword(s):  
Electron Microscopy ◽  
Transferrin Receptor ◽  
Structural Model ◽  
Human Transferrin ◽  
Human Transferrin Receptor
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