Ionic Permeability of Brain Microvessels

2015 ◽  
pp. 20-31
Author(s):  
S. -P. Olesen
Keyword(s):  
Ionic Permeability ◽  
Brain Microvessels

scholarly journals Rho-kinase inhibitor hydroxyfasudil protects against HIV-1 Tat-induced dysfunction of tight junction and neprilysin/Aβ transfer receptor expression in mouse brain microvessels

2021 ◽  
Vol 476 (5) ◽  
pp. 2159-2170
Author(s):  
Qiangtang Chen ◽  
Yu Wu ◽  
Yachun Yu ◽  
Junxiang Wei ◽  
Wen Huang
Keyword(s):  
Tight Junction ◽  
Signaling Pathway ◽  
Mouse Brain ◽  
Kinase Inhibitor ◽  
Rho Kinase ◽  
Receptor Expression ◽  
Mrna Levels ◽  
Brain Microvessels ◽  
Rho Kinase Inhibitor ◽  
Hiv 1

AbstractHIV-1 transactivator protein (Tat) induces tight junction (TJ) dysfunction and amyloid-beta (Aβ) clearance dysfunction, contributing to the development and progression of HIV-1-associated neurocognitive disorder (HAND). The Rho/ROCK signaling pathway has protective effects on neurodegenerative disease. However, the underlying mechanisms of whether Rho/ROCK protects against HIV-1 Tat-caused dysfunction of TJ and neprilysin (NEP)/Aβ transfer receptor expression have not been elucidated. C57BL/6 mice were administered sterile saline (i.p., 100 μL) or Rho-kinase inhibitor hydroxyfasudil (HF) (i.p., 10 mg/kg) or HIV-1 Tat (i.v., 100 μg/kg) or HF 30 min before being exposed to HIV-1 Tat once a day for seven consecutive days. Evans Blue (EB) leakage was detected via spectrophotometer and brain slides in mouse brains. The protein and mRNA levels of zonula occludens-1 (ZO-1), occludin, NEP, receptor for advanced glycation end products (RAGE), and low-density lipoprotein receptor-related protein 1 (LRP1) in mouse brain microvessels were, respectively, analyzed by Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) analyses. Exposure of the mice to HIV-1 Tat increased the amount of EB leakage, EB fluorescence intensity, blood–brain barrier (BBB) permeability, as well as the RAGE protein and mRNA levels, and decreased the protein and mRNA levels of ZO-1, occludin, NEP, and LRP1 in mouse brain microvessels. However, these effects were weakened by Rho-kinase inhibitor HF. Taken together, these results provide information that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-induced dysfunction of TJ and NEP/Aβ transfer receptor expression in the C57BL/6 mouse brain. These findings shed some light on potentiality of inhibiting Rho/Rock signaling pathway in handling HAND.

scholarly journals Effect of ammonia on amino acid uptake by brain microvessels.

1984 ◽  
Vol 259 (8) ◽  
pp. 5295-5300
Author(s):  
P Cardelli-Cangiano ◽  
C Cangiano ◽  
J H James ◽  
F Ceci ◽  
J E Fischer ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Brain Microvessels

Arachidonoyl-Coenzyme A Synthetase and Nonspecific AcylCoenzyme A Synthetase Activities in Purified Rat Brain Microvessels

1987 ◽  
Vol 48 (4) ◽  
pp. 1150-1156 ◽  
Author(s):  
O. Morand ◽  
J. B. Carré ◽  
P Homayoun ◽  
E Niel ◽  
N. Baumann ◽  
...  
Keyword(s):  
Rat Brain ◽  
Coenzyme A ◽  
Brain Microvessels

Enzymic activity of the brain: microvessels vs. total forebrain homogenate

1977 ◽  
Vol 138 (3) ◽  
pp. 561-564 ◽  
Author(s):  
B.M. Djuricˇic´ ◽  
B.B. Mruˇlja
Keyword(s):  
Enzymic Activity ◽  
Brain Microvessels ◽  
The Brain

Expression of receptors for native and chemically modified low-density lipoproteins in brain microvessels

FEBS Letters ◽  
1997 ◽  
Vol 401 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Marco Lucarelli ◽  
Massimo Gennarelli ◽  
Patrizia Cardelli ◽  
Giuseppe Novelli ◽  
Sigfrido Scarpa ◽  
...  
Keyword(s):  
Low Density Lipoproteins ◽  
Low Density ◽  
Chemically Modified ◽  
Brain Microvessels ◽  
Modified Low Density Lipoproteins

scholarly journals Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels

2015 ◽  
Vol 134 (2) ◽  
pp. 200-210 ◽  
Author(s):  
Margaret E. Tome ◽  
Charles P. Schaefer ◽  
Leigh M. Jacobs ◽  
Yifeng Zhang ◽  
Joseph M. Herndon ◽  
...  
Keyword(s):  
Rat Brain ◽  
Specific Binding ◽  
Binding Partners ◽  
Brain Microvessels ◽  
P Glycoprotein

scholarly journals Platelet Accumulation in Brain Microvessels in Fatal Pediatric Cerebral Malaria

2003 ◽  
Vol 187 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Georges E. Grau ◽  
Charles D. Mackenzie ◽  
Richard A. Carr ◽  
Mireille Redard ◽  
Giampaolo Pizzolato ◽  
...  
Keyword(s):  
Cerebral Malaria ◽  
Brain Microvessels ◽  
Platelet Accumulation
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