Islet Transplantation in the Cohen Diabetic Rat

2015 ◽  
pp. 184-200
Author(s):  
K. Federlin ◽  
S. Wiegand ◽  
R. G. Bretzel ◽  
D. Fritsch ◽  
R. Kaiser ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Diabetic Rat

scholarly journals Islet Transplantation Attenuating Testicular Injury in Type 1 Diabetic Rats Is Associated with Suppression of Oxidative Stress and Inflammation via Nrf-2/HO-1 and NF-κB Pathways

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Xiandong Zhu ◽  
Feixia Guo ◽  
Hengjie Tang ◽  
Chongchu Huang ◽  
Gangyin Xie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Islet Transplantation ◽  
Group Versus ◽  
Myocardial Damage ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Testicular Damage ◽  
Sod Activity ◽  
Respective Treatment ◽  
Testicular Injury

Testicular structural and functional impairment is a serious complication in male diabetes mellitus (DM) patients that leads to impaired fertility in adulthood. In contrast to other endocrine therapies, islet transplantation (IT) can effectively prevent and even reverse diabetic nephropathy and myocardial damage. However, whether IT can alleviate diabetes-induced testicular injury remains unclear. In this study, we sought to investigate the effect of IT on diabetes-induced testicular damage. A diabetic rat model was established by streptozotocin injection. DM, IT, and insulin treatment (INS) groups were compared after 4 weeks of respective treatment. We confirmed that IT could effectively attenuate diabetes-induced testicular damage and recover sperm counts more extensively compared with INS in diabetic rats. In addition, significantly higher levels of superoxide dismutase (SOD) activity and lower contents of malondialdehyde (MDA) were detected in the testes of the IT group versus diabetic rats. Mechanism studies revealed that IT significantly activates the expression of Nrf-2, HO-1, and NQO-1 and inhibits upregulation of the NF-κB expression in response to DM, while INS only exhibit slight impact on the protein expression. Therefore, we speculate that IT may prevent the progression of testicular damage by downregulating oxidative stress and inhibiting inflammation via Nrf-2/HO-1 and NF-κB pathways.

ASSESSMENT OF PROTEINURIA AND NEUROPATHY IN THE NONIMMUNOSUPPRESSED BB DIABETIC RAT AFTER ABDOMINAL INTRATESTICULAR ISLET TRANSPLANTATION

1993 ◽  
Vol 56 (3) ◽  
pp. 680-686 ◽  
Author(s):  
FREDERICK T. MURRAY ◽  
ANNETTE BEYER-MEARS ◽  
RICHARD D. JOHNSON ◽  
ANDERS A. F. SIMA ◽  
DON F. CAMERON ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Diabetic Rat

scholarly journals Mesenteric lymph nodes as alternative site for pancreatic islet transplantation in a diabetic rat model

BMC Surgery ◽  
2019 ◽  
Vol 18 (S1) ◽  
Author(s):  
Massimiliano Veroux ◽  
Rita Bottino ◽  
Roberta Santini ◽  
Suzanne Bertera ◽  
Daniela Corona ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Islet Transplantation ◽  
Pancreatic Islet ◽  
Rat Model ◽  
Diabetic Rat ◽  
Pancreatic Islet Transplantation ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Alternative Site ◽  
Diabetic Rat Model

Islet transplantation improves glucose oxidation and mechanical function in diabetic rat hearts

1993 ◽  
Vol 71 (12) ◽  
pp. 896-903 ◽  
Author(s):  
G. D. Lopaschuk ◽  
J. R. T. Lakey ◽  
R. Barr ◽  
R. Wambolt ◽  
A. B. R. Thomson ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Glucose Oxidation ◽  
Heart Function ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Mechanical Function ◽  
Oxidation Rates ◽  
Rat Hearts ◽  
Low Glucose ◽  
Developed Pressure

In poorly controlled diabetes an impairment of glucose use can contribute to a depression in mechanical function of rat hearts. In this study we determined the effects of islet transplantation on glucose use and heart function in streptozotocin-induced diabetic rats. Myocardial function, glycolysis, and glucose oxidation were measured in isolated working hearts obtained from control, diabetic, and islet-transplanted diabetic Wistar–Furth rats. Islets (1200) were transplanted beneath the kidney capsule 2 weeks after a single i.v. dose of streptozotocin (55 mg/kg). The study consisted of three groups: (i) islet-transplanted diabetic rats, (ii) untreated diabetic controls, and (iii) normal controls. Following 11 weeks of monitoring, working hearts were perfused at a 11.5-mmHg (1 mmHg = 133.3 Pa) preload and 80-mmHg afterload, with buffer containing 11 mM [5-3H, 14C(U)]glucose, 1.2 mM palmitate, and 100 μU/mL insulin. In untreated diabetic rat hearts, glucose oxidation rates were markedly depressed compared with control hearts (30.4 ± 4 and 510 ± 68 nmol∙g−1 dry wt.∙min−1, respectively). Low glucose oxidation rates in diabetic rats were significantly improved in islet-transplanted animals (234 ± 39 nmol∙g−1 dry wt.∙min−1). The low glucose oxidation rates in untreated diabetic rat hearts were accompanied by an impaired mechanical function compared with control hearts, which was improved by islet transplantation (heart rate × developed pressure × 10−3 was 10.6 ± 0.9, 14.8 ± 1.3, and 14.8 ± 1.5 beats∙mmHg∙min−1, respectively). In the presence of insulin, steady-state rates of glycolysis were only slightly depressed in untreated diabetic rat hearts compared with control (1944 ± 436 and 2720 ± 265 nmol∙g−1 dry wt.∙min−1, respectively). However, during a reduction of coronary flow to 0.5 mL∙min−1, glycolytic rates accelerated in control and islet-transplanted rat hearts, but not in untreated diabetic rat hearts. These data show that the decrease in glucose use that occurs in untreated diabetic rats under both aerobic and ischemic conditions can be significantly alleviated by islet transplantation. The increase in glucose oxidation in aerobic hearts supports our previous studies, which suggest that increasing glucose oxidation can improve function in diabetic rat hearts.Key words: glucose oxidation, glycolysis, diabetes, islet transplantation.

Reduction of marginal mass required for successful islet transplantation in a diabetic rat model using adipose tissue–derived mesenchymal stromal cells

Cytotherapy ◽  
2018 ◽  
Vol 20 (9) ◽  
pp. 1124-1142 ◽  
Author(s):  
Mona Navaei-Nigjeh ◽  
Milad Moloudizargari ◽  
Maryam Baeeri ◽  
Mahdi Gholami ◽  
Nasrin Lotfibakhshaiesh ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Islet Transplantation ◽  
Mesenchymal Stromal Cells ◽  
Rat Model ◽  
Stromal Cells ◽  
Diabetic Rat ◽  
Diabetic Rat Model

scholarly journals The effect of insulin treatment and of islet transplantation on the resistance artery function in the STZ-induced diabetic rat

1996 ◽  
Vol 119 (3) ◽  
pp. 495-504 ◽  
Author(s):  
Katherine M. Heygate ◽  
Joanne Davies ◽  
Michael Holmes ◽  
Roger F.L. James ◽  
Herbert Thurston
Keyword(s):  
Islet Transplantation ◽  
Insulin Treatment ◽  
Diabetic Rat ◽  
Resistance Artery

Troglitazone prevents falty changes of the liver in obese diabetic rat

2001 ◽  
Vol 120 (5) ◽  
pp. A207-A208
Author(s):  
D JIA ◽  
T AKIYAMA ◽  
K FUKUMITSU ◽  
M YAMAMOTO ◽  
S ABE ◽  
...  
Keyword(s):  
Diabetic Rat

Effects of empagliflozin on oxidative stress and endothelial dysfunction in STZ-induced Type 1 diabetic rat

2014 ◽  
Vol 9 (S 01) ◽  
Author(s):  
M Oelze ◽  
S Kröller-Schön ◽  
M Mader ◽  
E Zinßius ◽  
P Stamm ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Diabetic Rat
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