Influence of Thyroid-Stimulating Hormone, Epidermal Growth Factor, and Insulin-Like Growth Factor I on Growth of Thyroid Cells in vitro

2015 ◽  
pp. 88-97 ◽  
Author(s):  
H. Schatz ◽  
R. Freiberger ◽  
C. Richter ◽  
F. Wiss ◽  
K. Weber
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Thyroid Stimulating Hormone ◽  
Insulin Like Growth Factor ◽  
Thyroid Cells ◽  
Factor I ◽  
Epidermal Growth ◽  
Growth Factor I ◽  
Stimulating Hormone

scholarly journals Stimulatory effect of insulin-like growth factor I and epidermal growth factor on the maturation of rabbit oocytes in vitro

Reproduction ◽  
1996 ◽  
Vol 107 (1) ◽  
pp. 109-117 ◽  
Author(s):  
P. L. Lorenzo ◽  
P. G. Rebollar ◽  
M. J. Illera ◽  
J. C. Illera ◽  
M. Illera ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Epidermal Growth ◽  
Growth Factor I

Effects of insulin-like growth factor-I, epidermal growth factor and cysteamine on the in vitro maturation and development of oocytes collected from 6- to 8-week-old Merino lambs

2008 ◽  
Vol 20 (5) ◽  
pp. 570 ◽  
Author(s):  
Jennifer M. Kelly ◽  
David O. Kleemann ◽  
W. M. Chis Maxwell ◽  
Simon K. Walker
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Embryo Development ◽  
Insulin Like Growth Factor ◽  
Embryo Viability ◽  
Factor I ◽  
Igf I ◽  
Epidermal Growth ◽  
Growth Factor I

To improve the viability of embryos produced in vitro from lamb oocytes, maturation medium was supplemented with insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), cysteamine, and combinations thereof. Experiment 1 examined the effects of IGF-I supplementation and duration of oocyte maturation on nuclear maturation and embryo development while Experiments 2 and 3 examined the effects of cysteamine and EGF supplementation respectively on embryo development. In Experiment 4, embryo development was examined after maturation with various combinations of supplements. IGF-I supplementation increased cleavage rate (P < 0.05) but its effect on the rate of blastocyst production from original oocytes was variable. Supplementation with IGF-I increased (P < 0.01) the proportion of oocytes at Metaphase II (MII) after 18 h of maturation but not at later times. EGF either alone or combined with IGF-I significantly (P < 0.05) increased cleavage rates compared with other treatment groups but EGF consistently failed to improve blastocyst production rates. Cysteamine improved hatching rates but only when supplemented alone. Maturation of lamb oocytes for 22 h in medium supplemented with 100 ng mL–1 IGF-I and 100 μm cysteamine resulted in the production of 16.0 lambs per donor lamb after embryos were transferred to recipient ewes. It is concluded that EGF and, to a lesser extent, IGF-I, whilst beneficial to initial cleavage, can adversely influence subsequent embryo development. Improvements in embryo viability may more likely be obtained by addressing issues that influence fetal oocyte quality than by modifying in vitro methodology.

Role of epidermal growth factor and insulin-like growth factor-I on porcine oocyte maturation and embryonic development in vitro

1997 ◽  
Vol 9 (6) ◽  
pp. 571 ◽  
Author(s):  
Christopher G. Grupen ◽  
Hiroshi Nagashima ◽  
Mark B. Nottle
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Embryonic Development ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Maturation Medium ◽  
Igf I ◽  
Epidermal Growth ◽  
Growth Factor I

The effects of epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) on the in vitro maturation of porcine oocytes were examined. Oocytes obtained from the ovaries of slaughtered prepubertal gilts were matured in modified Medium 199 supplemented with 25% porcine follicular ßuid and gonadotropins, and fertilized in vitro. Oocytes were either xed 16 h later to assess fertilization or cultured for 7 days to assess embryonic development. In Experiment 1, the addition of EGF to maturation medium increased the percentage of meiotically mature oocytes (88% v. 70%; P < 0· 001) but did not affect the proportion of fertilized or cleaved oocytes. Blastocysts derived from oocytes matured in medium supplemented with 10 ng mL-1 EGF had a greater number of cells compared with those of control blastocysts (51·1 ± 5· 1 v. 36·0 ± 3·1; P < 0· 02). In Experiment 2, the addition of IGF-I to maturation medium had no effect on meiotic maturation, fertilization or embryonic development. Our ndings demonstrate that EGF plays an important role in both the meiotic and cytoplasmic maturation of porcine oocytes in vitro.

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