Generation, Selection, Properties and Quality Control of Murine Monoclonal Antibodies

1988 ◽  
pp. 20-37
Keyword(s):  
Quality Control ◽  
Monoclonal Antibodies ◽  
Murine Monoclonal Antibodies

scholarly journals Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wenbo Jiang ◽  
Julius Wong ◽  
Hyon-Xhi Tan ◽  
Hannah G. Kelly ◽  
Paul G. Whitney ◽  
...  
Keyword(s):  
Animal Model ◽  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Cross Reactivity ◽  
Tfh Cells ◽  
Follicular Helper ◽  
Lymph Node Cells ◽  
Human Viruses ◽  
Humoral Responses ◽  
Murine Monoclonal Antibodies

AbstractThe ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre‐clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret‐reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2.

Generation and Characterization of Murine Monoclonal Antibodies anti-PBP2a of Methicillin-resistantStaphylococcus aureus

2015 ◽  
Vol 34 (4) ◽  
pp. 257-262 ◽  
Author(s):  
José Procópio M. Senna ◽  
Maria da Glória M. Teixeira ◽  
Marta de A. Santiago ◽  
Nádia M. Batoréu ◽  
Napoleão Valadares ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Murine Monoclonal Antibodies

Characterization of Murine Monoclonal Antibodies to HIV-1 Induced by Synthetic Peptides

1988 ◽  
Vol 4 (5) ◽  
pp. 331-342 ◽  
Author(s):  
PAUL J. DURDA ◽  
BARBARA LEECE ◽  
ANNA JENOSKI ◽  
HARVEY RABIN ◽  
AMANDA FISHER ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Murine Monoclonal Antibodies ◽  
Hiv 1

Characterization of Novel Murine Monoclonal Antibodies Directed Against the Extracellular Domain of Human HER2 Tyrosine Kinase Receptor

Hybridoma ◽  
2011 ◽  
Vol 30 (4) ◽  
pp. 347-353 ◽  
Author(s):  
Tohid Kazemi ◽  
Fathollah Tahmasebi ◽  
Ali Ahmad Bayat ◽  
Neda Mohajer ◽  
Jalal Khoshnoodi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tyrosine Kinase ◽  
Extracellular Domain ◽  
Tyrosine Kinase Receptor ◽  
Murine Monoclonal Antibodies
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