Mechanism of Cytotoxic Activity of Tumor Necrosis Factor and Changes in Serum Factors following Administration of TNF

2015 ◽  
pp. 59-70
Author(s):  
Nobuko Satomi ◽  
Akiko Sakurai ◽  
Ruriko Haranaka ◽  
Katsuyuki Haranaka
Keyword(s):  
Tumor Necrosis Factor ◽  
Cytotoxic Activity ◽  
Tumor Necrosis ◽  
Serum Factors ◽  
Necrosis Factor

Tumor Necrosis Factor Mutants with Selective Cytotoxic Activity

1999 ◽  
Vol 23 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Nadia Berkova ◽  
Andre Lemay ◽  
Vyatcheslav Korobko ◽  
Ludmila Shingarova ◽  
Lubov Sagaidak ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Cytotoxic Activity ◽  
Tumor Necrosis ◽  
Necrosis Factor

The Induction of Nitric Oxide by Interleukin-12 and Tumor Necrosis Factor- in Human Natural Killer Cells: Relationship With the Regulation of Lytic Activity

Blood ◽  
1998 ◽  
Vol 92 (6) ◽  
pp. 2093-2102 ◽  
Author(s):  
Ombretta Salvucci ◽  
Jean Pierre Kolb ◽  
Bernard Dugas ◽  
Nathalie Dugas ◽  
Salem Chouaib
Keyword(s):  
Nitric Oxide ◽  
Tumor Necrosis Factor ◽  
Cytotoxic Activity ◽  
Natural Killer ◽  
Tumor Necrosis ◽  
Nk Cell ◽  
Lytic Activity ◽  
Interleukin 12 ◽  
Target Cells ◽  
Necrosis Factor

Abstract We have investigated the interleukin-12 (IL-12) and tumor necrosis factor- (TNF)-induced regulation of human natural killer (NK) cell function and their relationship with nitric oxide (NO) generation. We demonstrate that both cytokines were efficient to trigger the transcription of the inducible nitric oxide synthase (iNOS) mRNA, as detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Western blot analysis and intracytoplasmic fluorescence showed that iNOS protein was also induced by both cytokines. However, our data indicate that NO does not play a significant role in the effector phase of the cytotoxic activity mediated by NK-stimulated cells, inasmuch as the lytic activity was not affected in the presence of specific NO synthase inhibitors. When aminoguanidine (AMG), an inhibitor of iNOS, was added during the afferent phase of NK stimulation with IL-12 and TNF, a subsequent increase in the lytic potential of the effector cells towards the NK-sensitive target cells (K562) and lymphokine-activated killer (LAK) target cells (Daudi) was observed. Conversely, the addition of chemical NO donors during the afferent step resulted in a dose-dependent inhibition of the NK and LAK cytotoxicity. Our data suggest that the enhancement of NK-cell cytotoxic activity resulting from iNOS inhibition may be correlated, at least in part, to an increase in interferon-γ production and granzyme B expression. © 1998 by The American Society of Hematology.

The Induction of Nitric Oxide by Interleukin-12 and Tumor Necrosis Factor- in Human Natural Killer Cells: Relationship With the Regulation of Lytic Activity

Blood ◽  
1998 ◽  
Vol 92 (6) ◽  
pp. 2093-2102 ◽  
Author(s):  
Ombretta Salvucci ◽  
Jean Pierre Kolb ◽  
Bernard Dugas ◽  
Nathalie Dugas ◽  
Salem Chouaib
Keyword(s):  
Nitric Oxide ◽  
Tumor Necrosis Factor ◽  
Cytotoxic Activity ◽  
Natural Killer ◽  
Tumor Necrosis ◽  
Nk Cell ◽  
Lytic Activity ◽  
Interleukin 12 ◽  
Target Cells ◽  
Necrosis Factor

We have investigated the interleukin-12 (IL-12) and tumor necrosis factor- (TNF)-induced regulation of human natural killer (NK) cell function and their relationship with nitric oxide (NO) generation. We demonstrate that both cytokines were efficient to trigger the transcription of the inducible nitric oxide synthase (iNOS) mRNA, as detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Western blot analysis and intracytoplasmic fluorescence showed that iNOS protein was also induced by both cytokines. However, our data indicate that NO does not play a significant role in the effector phase of the cytotoxic activity mediated by NK-stimulated cells, inasmuch as the lytic activity was not affected in the presence of specific NO synthase inhibitors. When aminoguanidine (AMG), an inhibitor of iNOS, was added during the afferent phase of NK stimulation with IL-12 and TNF, a subsequent increase in the lytic potential of the effector cells towards the NK-sensitive target cells (K562) and lymphokine-activated killer (LAK) target cells (Daudi) was observed. Conversely, the addition of chemical NO donors during the afferent step resulted in a dose-dependent inhibition of the NK and LAK cytotoxicity. Our data suggest that the enhancement of NK-cell cytotoxic activity resulting from iNOS inhibition may be correlated, at least in part, to an increase in interferon-γ production and granzyme B expression. © 1998 by The American Society of Hematology.

Inhibitory effect of suramin on receptor binding and cytotoxic activity of tumor necrosis factor α

1992 ◽  
Vol 14 (4) ◽  
pp. 637-642 ◽  
Author(s):  
Laura Grazioli ◽  
Rachele Alzani ◽  
Marina Ciomei ◽  
Mariangela Mariani ◽  
Alessandra Restivo ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Cytotoxic Activity ◽  
Receptor Binding ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Inhibitory Effect ◽  
Factor Α ◽  
Necrosis Factor
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