Histochemical Analysis of the Lizard Cortex: An Acetylcholinesterase, Cytochrome Oxidase and NADPH-Diaphorase Study

2015 ◽  
pp. 77-84 ◽  
Author(s):  
Jos� Regidor ◽  
Lidia Poch
Author(s):  
C.D. Vargas ◽  
A.O. Sousa ◽  
F.L.R. Bittencourt ◽  
C.M. Santos ◽  
A. Pereira ◽  
...  

Author(s):  
W. A. Shannon ◽  
M. A. Matlib

Numerous studies have dealt with the cytochemical localization of cytochrome oxidase via cytochrome c. More recent studies have dealt with indicating initial foci of this reaction by altering incubation pH (1) or postosmication procedure (2,3). The following study is an attempt to locate such foci by altering membrane permeability. It is thought that such alterations within the limits of maintaining morphological integrity of the membranes will ease the entry of exogenous substrates resulting in a much quicker oxidation and subsequently a more precise definition of the oxidative reaction.The diaminobenzidine (DAB) method of Seligman et al. (4) was used. Minced pieces of rat liver were incubated for 1 hr following toluene treatment (5,6). Experimental variations consisted of incubating fixed or unfixed tissues treated with toluene and unfixed tissues treated with toluene and subsequently fixed.


1992 ◽  
Vol 1101 (2) ◽  
pp. 192-194
Author(s):  
G BABCOCK ◽  
C VAROTSIS ◽  
Y ZHANG

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