Molecular Studies of a Positive Regulator of Toxin A Synthesis in Pseudomonas aeruginosa

2015 ◽  
pp. 279-289 ◽  
Author(s):  
Michael S. Hindahl ◽  
Dara W. Frank ◽  
Barbara H. Iglewski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Toxin A ◽  
Positive Regulator ◽  
Molecular Studies

Toxoids of Pseudomonas aeruginosa Toxin A: Production by Chemical and Genetic Means

1983 ◽  
Vol 5 (Supplement_5) ◽  
pp. S992-S997 ◽  
Author(s):  
Stanley J. Cryz ◽  
Barbara H. Iglewski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Toxin A

Corneal Infections in Mice with Toxin A and Elastase Mutants of Pseudomonas aeruginosa

1980 ◽  
Vol 142 (4) ◽  
pp. 547-555 ◽  
Author(s):  
D. E. Ohman ◽  
R. P. Burns ◽  
B. H. Iglewski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Toxin A

scholarly journals Functional Analysis of PvdS, an Iron Starvation Sigma Factor of Pseudomonas aeruginosa

2000 ◽  
Vol 182 (6) ◽  
pp. 1481-1491 ◽  
Author(s):  
Livia Leoni ◽  
Nicola Orsi ◽  
Victor de Lorenzo ◽  
Paolo Visca
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sigma Factor ◽  
Sigma Factors ◽  
Gene Copy ◽  
Wild Type ◽  
Iron Starvation ◽  
E Coli ◽  
Positive Regulator ◽  
Functional Features

ABSTRACT In Pseudomonas aeruginosa, iron modulates gene expression through a cascade of negative and positive regulatory proteins. The master regulator Fur is involved in iron-dependent repression of several genes. One of these genes, pvdS, was predicted to encode a putative sigma factor responsible for the transcription of a subset of genes of the Fur regulon. PvdS appears to belong to a structurally and functionally distinct subgroup of the extracytoplasmic function family of alternative sigma factors. Members of this subgroup, also including PbrA from Pseudomonas fluorescens, PfrI and PupI from Pseudomonas putida, and FecI from Escherichia coli, are controlled by the Fur repressor, and they activate transcription of genes for the biosynthesis or the uptake of siderophores. Evidence is provided that the PvdS protein of P. aeruginosa is endowed with biochemical properties of eubacterial sigma factors, as it spontaneously forms 1:1 complexes with the core fraction of RNA polymerase (RNAP, α2ββ′ subunits), thereby promoting in vitro binding of the PvdS-RNAP holoenzyme to the promoter region of the pvdA gene. These functional features of PvdS are consistent with the presence of structural domains predicted to be involved in core RNAP binding, promoter recognition, and open complex formation. The activity of pyoverdin biosynthetic (pvd) promoters was significantly lower in E. coli overexpressing the multicopy pvdS gene than in wild-type P. aeruginosa PAO1 carrying the single gene copy, andpvd::lacZ transcriptional fusions were silent in both pfrI (the pvdS homologue) and pfrA (a positive regulator of pseudobactin biosynthetic genes) mutants of P. putida WCS358, while they are expressed at PAO1 levels in wild-type WCS358. Moreover, the PvdS-RNAP holoenzyme purified from E. coli lacked the ability to generate in vitro transcripts from the pvdA promoter. These observations suggest that at least one additional positive regulator could be required for full activity of the PvdS-dependent transcription complex both in vivo and in vitro. This is consistent with the presence of a putative activator binding site (the iron starvation box) at variable distance from the transcription initiation sites of promoters controlled by the iron starvation sigma factors PvdS, PfrI, and PbrA of fluorescent pseudomonads.

scholarly journals The effect of Pseudomonas aeruginosa cytotoxin and toxin A on human polymorphonuclear leukocytes

1987 ◽  
Vol 24 (4) ◽  
pp. 315-324 ◽  
Author(s):  
M. B. Bishop ◽  
A. L. Baltch ◽  
L. A. Hill ◽  
R. P. Smith ◽  
F. Lutz ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Polymorphonuclear Leukocytes ◽  
Toxin A ◽  
Human Polymorphonuclear Leukocytes

scholarly journals Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats.

1982 ◽  
Vol 36 (3) ◽  
pp. 1223-1228 ◽  
Author(s):  
D E Woods ◽  
S J Cryz ◽  
R L Friedman ◽  
B H Iglewski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Toxin A ◽  
Lung Infections

scholarly journals Safety and immunogenicity of a Pseudomonas aeruginosa O-polysaccharide toxin A conjugate vaccine in humans.

1987 ◽  
Vol 80 (1) ◽  
pp. 51-56 ◽  
Author(s):  
S J Cryz ◽  
E Fürer ◽  
A S Cross ◽  
A Wegmann ◽  
R Germanier ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Conjugate Vaccine ◽  
Toxin A
Sign in / Sign up

Export Citation Format

Share Document