The Pharmacology of Anticonvulsants: Studies of Epileptiform Activity in the Hippocampal Slice Preparation

2015 ◽  
pp. 184-188 ◽  
Author(s):  
David Ashton ◽  
Roland Willems ◽  
Erik De Prins ◽  
Albert Wauquier
Keyword(s):  
Hippocampal Slice ◽  
Epileptiform Activity ◽  
Slice Preparation

Folic acid has a disinhibitory action in the rat hippocampal slice preparation

1985 ◽  
Vol 346 (2) ◽  
pp. 281-286 ◽  
Author(s):  
Laura C. Otis ◽  
Daniel V. Madison ◽  
Roger A. Nicoll
Keyword(s):  
Folic Acid ◽  
Hippocampal Slice ◽  
Slice Preparation

The Hippocampal Slice Preparation

2019 ◽  
pp. 211-226
Author(s):  
Larry G. Stark ◽  
Timothy E. Albertson
Keyword(s):  
Hippocampal Slice ◽  
Slice Preparation

Epileptiform activity induced by low chloride medium in the CA1 subfield of the hippocampal slice

1990 ◽  
Vol 64 (6) ◽  
pp. 1747-1757 ◽  
Author(s):  
M. Avoli ◽  
C. Drapeau ◽  
P. Perreault ◽  
J. Louvel ◽  
R. Pumain
Keyword(s):  
Membrane Potential ◽  
Large Amplitude ◽  
Action Potentials ◽  
Hippocampal Slice ◽  
Epileptiform Activity ◽  
Field Potential ◽  
Pyramidal Cells ◽  
Input Resistance ◽  
Field Potentials ◽  
Maximal Amplitude

1. Extracellular and intracellular recordings and measurements of the extracellular concentration of free K+ ([K+]o) were performed in the CA1 subfield of the rat hippocampal slice during perfusion with artificial cerebrospinal fluid (ACSF) in which NaCl had been replaced with equimolar Na-isethionate or Na-methylsulfate (hereafter called low Cl- ACSF). 2. CAl pyramidal cells perfused with low Cl- ACSF generated intracellular epileptiform potentials in response to orthodromic, single-shock stimuli delivered in stratum (S.) radiatum. Low-intensity stimuli evoked a short-lasting epileptiform burst (SB) of action potentials that lasted 40–150 ms and was followed by a prolonged hyperpolarization. When the stimulus strength was increased, a long-lasting epileptiform burst (LB) appeared; it had a duration of 4–15 s and consisted of an early discharge of action potentials similar to the SB, followed by a prolonged, large-amplitude depolarizing plateau. The refractory period of the LB was longer than 20 s. SB and LB were also seen after stimulation of the alveus. 3. Variations of the membrane potential with injection of steady. DC current modified the shape of SB and LB. When microelectrodes filled with the lidocaine derivative QX-314 were used, the amplitudes of both SB and LB increased in a linear fashion during changes of the baseline membrane potential in the hyperpolarizing direction. The membrane input resistance, as measured by injecting brief square pulses of hyperpolarizing current, decreased by 65-80% during the long-lasting depolarizing plateau of LB. 4. A synchronous field potential and a transient increase in [K+]o accompanied the epileptiform responses. The extracellular counterpart of the SB was a burst of three to six population spikes and a small increase in [K+]o (less than or equal to 2 mM from a resting value of approximately 2.5 mM). The LB was associated with a large-amplitude, biphasic, negative field potential and a large increase in [K+]o (up to 12.4 mM above the resting value). Changes in [K+]o during the LB were largest at the border between S. oriens and S. pyramidale. This was also the site where the field potentials measured 2–5 s after the stimulus attained their maximal amplitude. Conversely, field potentials associated with the early component of the LB or with the SB displayed a maximal amplitude in the S. radiatum. 5. Spontaneous SBs and LBs were at times recorded in the CA1 and in the CA3 subfield.(ABSTRACT TRUNCATED AT 400 WORDS)

Dopaminergic and noradrenergic responses in the hippocampal slice preparation

1984 ◽  
Vol 23 (3) ◽  
pp. 303-307 ◽  
Author(s):  
M.G. Marciani ◽  
P. Calabresi ◽  
P. Stanzione ◽  
G. Bernardi
Keyword(s):  
Hippocampal Slice ◽  
Slice Preparation

Epileptiform activity in hippocampal slice cultures with normal inhibitory synaptic drive

1990 ◽  
Vol 108 (1-2) ◽  
pp. 76-80 ◽  
Author(s):  
Alfred T. Malouf ◽  
Carol A. Robbins ◽  
Philip A. Schwartzkroin
Keyword(s):  
Hippocampal Slice ◽  
Epileptiform Activity ◽  
Hippocampal Slice Cultures ◽  
Synaptic Drive
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