Mechanisms of Lymphocyte Adhesion to Endothelial Cells

2015 ◽  
pp. 119-122
Author(s):  
Dorian O. Haskard ◽  
Druie Cavender ◽  
Morris Ziff
Keyword(s):  
Endothelial Cells ◽  
Lymphocyte Adhesion

Human lymphocyte adhesion to xenogeneic porcine endothelial cells: modulation by human TNF-α and involvement of VLA-4 and LFA-1

1996 ◽  
Vol 4 (4) ◽  
pp. 265-270 ◽  
Author(s):  
Beatrice Birmele ◽  
Gilles Thibault ◽  
Hubert Nivet ◽  
Yves Gruel ◽  
Pierre Bardos ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Lymphocyte ◽  
Lymphocyte Adhesion ◽  
Tnf Α ◽  
Porcine Endothelial Cells

Syn- capping of human T lymphocyte adhesion/activation molecules and their redistribution during interaction with endothelial cells

1993 ◽  
Vol 53 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Stephen J. Rosenman ◽  
Amir A. Ganji ◽  
Thomas F. Tedder ◽  
W. Michael Gallatin
Keyword(s):  
Endothelial Cells ◽  
T Lymphocyte ◽  
Lymphocyte Adhesion ◽  
Human T Lymphocyte

scholarly journals Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells.

1988 ◽  
Vol 107 (1) ◽  
pp. 321-331 ◽  
Author(s):  
M L Dustin ◽  
T A Springer
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Adhesion Molecule ◽  
Umbilical Vein ◽  
Peripheral Blood Lymphocytes ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Lymphocyte Function ◽  
Intercellular Adhesion Molecule 1 ◽  
Lymphocyte Adhesion

Intercellular adhesion molecule-1 (ICAM-1) on the surface of cultured umbilical vein and saphenous vein endothelial cells was upregulated between 2.5- and 40-fold by rIL-1, rTNF, LPS and rIFN gamma corresponding to up to 5 X 10(6) sites/cell. Endothelial cell ICAM-1 was a single band of 90 kD in SDS-PAGE. Purified endothelial cell ICAM-1 reconstituted into liposomes and bound to plastic was an excellent substrate for both JY B lymphoblastoid cell and T lymphoblast adhesion. Adhesion to endothelial cell ICAM-1 in planar membranes was blocked completely by monoclonal antibodies to lymphocyte function associated antigen-1 (LFA-1) or ICAM-1. Adhesion to artificial membranes was most sensitive to ICAM-1 density within the physiological range found on resting and stimulated endothelial cells. Adhesion of JY B lymphoblastoid cells, normal and genetically LFA-1 deficient T lymphoblasts and resting peripheral blood lymphocytes to endothelial cell monolayers was also assayed. In summary, LFA-1 dependent (60-90% of total adhesion) and LFA-1-independent basal adhesion was observed and the use of both adhesion pathways by different interacting cell pairs was increased by monokine or lipopolysaccharide stimulation of endothelial cells. The LFA-1-dependent adhesion could be further subdivided into an LFA-1/ICAM-1-dependent component which was increased by cytokines and a basal LFA-1-dependent, ICAM-1-independent component which did not appear to be affected by cytokines. We conclude that ICAM-1 is a regulated ligand for lymphocyte-endothelial cell adhesion, but at least two other major adhesion pathways exist.

Determination of T-lymphocyte adhesion to endothelial cells using a novel luminescence marker

1998 ◽  
Vol 3 (4) ◽  
pp. 245-252 ◽  
Author(s):  
C.C Termeer ◽  
J.M Weiss ◽  
H Dittmar ◽  
W Vanscheidt ◽  
E Schöpf ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
T Lymphocyte ◽  
Lymphocyte Adhesion

scholarly journals Lymphocyte adhesion to high endothelium is mediated by two beta 1 integrin receptors for fibronectin, alpha 4 beta 1 and alpha 5 beta 1

1992 ◽  
Vol 101 (4) ◽  
pp. 885-894 ◽  
Author(s):  
Z. Szekanecz ◽  
M.J. Humphries ◽  
A. Ager
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Peripheral Blood ◽  
Human Lymphocytes ◽  
In Vitro Assays ◽  
Lymphoid Tissues ◽  
Integrin Receptors ◽  
Lymphocyte Adhesion ◽  
Selective Expression ◽  
Beta 1 Integrin

Using a rat model we have previously proposed a role for fibronectin as an adhesive ligand on high endothelial cells (HEC) for recirculating lymphocytes. Lymphocyte adhesion to high endothelial cells was blocked by CS1 peptide (from the type III connecting segment of fibronectin) and RGD-containing peptides using two different in vitro assays of lymphocyte-HEC recognition, the frozen section assay and cultured HEC. In order to study the receptors utilised by lymphocytes to bind to HEC we have developed a xenogeneic model in which the adhesion of human lymphocytes to HEC cultured from rat lymph nodes is measured. The basic properties of lymphocyte-HEC interaction were retained using human lymphocytes. CS1 peptide and RGD-containing peptides gave similar profiles of inhibition of lymphocyte adhesion as found previously using rat cells. FACS analysis showed that the majority of peripheral blood lymphocytes expressed two beta 1 integrin receptors, alpha 4 beta 1 and alpha 5 beta 1, which are known to recognise distinct adhesion domains in fibronectin. A subpopulation of lymphocytes also expressed alpha 3 beta 1, which, like alpha 5 beta 1, has been reported to be an RGD-dependent adhesion receptor for the central cell binding domain of fibronectin. Anti-alpha 4 and anti-alpha 5 subunit monoclonal antibodies maximally inhibited adhesion to HEC by 60% and 65%, respectively. Monoclonal antibodies to the common beta 1 subunit gave slightly higher inhibition at 70%. These results suggest that lymphocytes employ one or both of two different beta 1 integrin fibronectin receptors to bind to HEC. The simultaneous or alternate engagement of two fibronectin receptors on the lymphocyte surface by immobilised fibronectin in the endothelial layer may contribute to the stabilisation of adhesive contacts or to the subsequent transendothelial migration of lymphocytes. In contrast to lymphocytes, peripheral blood neutrophils did not express any members of the beta 1 integrin family. The selective expression of beta 1 integrins by lymphocytes and not neutrophils contrasted with the widespread distribution of the other homing-associated adhesion molecules, LECAM-1, CD44 and LFA-1, on these two cell types. It is thus possible that the selective expression of beta 1 integrins regulates the constitutive migration of lymphocytes but not neutrophils into organised lymphoid tissues.

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