Preliminary Investigations of Tissue Specificity, Species Specificity, and Strategies for Identifying Chemicals Causing DNA Adducts in Human Placenta

2015 ◽  
pp. 86-103 ◽  
Author(s):  
Richard B. Everson ◽  
Erika Randerath ◽  
Tommie A. Avitts ◽  
Herman A. J. Schut ◽  
Kurt Randerath
Keyword(s):  
Dna Adducts ◽  
Human Placenta ◽  
Tissue Specificity ◽  
Species Specificity

Derivative fluorescence spectral analysis of polycyclic aromatic hydrocarbon-DNA adducts in human placenta

1989 ◽  
Vol 2 (2) ◽  
pp. 104-108 ◽  
Author(s):  
A. Weston ◽  
D. K. Manchester ◽  
M. C. Poirier ◽  
J. S. Choi ◽  
G. E. Trivers ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Dna Adducts ◽  
Human Placenta ◽  
Polycyclic Aromatic

DNA adducts in human placenta exposed to ambient environment and passive cigarette smoke during pregnancy

2007 ◽  
Vol 79 (4) ◽  
pp. 289-294 ◽  
Author(s):  
Mrinal K. Sanyal ◽  
Derya Mercan ◽  
Kathleen Belanger ◽  
Regina M. Santella
Keyword(s):  
Cigarette Smoke ◽  
Dna Adducts ◽  
Human Placenta ◽  
Ambient Environment

scholarly journals Detection of benzo[a]pyrene diol epoxide-DNA adducts in human placenta.

1988 ◽  
Vol 85 (23) ◽  
pp. 9243-9247 ◽  
Author(s):  
D. K. Manchester ◽  
A. Weston ◽  
J. S. Choi ◽  
G. E. Trivers ◽  
P. V. Fennessey ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Human Placenta ◽  
Diol Epoxide

P11 Catalytic activity, genotype of detoxifying enzymes and DNA-adducts in human placenta as risk factors for newborns

2004 ◽  
Vol 2 (1) ◽  
pp. 43
Author(s):  
N. Teplyuk ◽  
L. Sazonova ◽  
M. Obolenskaya ◽  
R. Divo ◽  
M. Poirier ◽  
...  
Keyword(s):  
Risk Factors ◽  
Catalytic Activity ◽  
Dna Adducts ◽  
Human Placenta ◽  
Detoxifying Enzymes

Detection of benzo[a]pyrene-DNA adducts in human placenta and umbilical cord blood

1997 ◽  
Vol 16 (12) ◽  
pp. 716-721 ◽  
Author(s):  
JP Arnould ◽  
P. Verhoest ◽  
V. Bach ◽  
JP Libert ◽  
J. Belegaud
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Dna Adducts ◽  
Umbilical Cord Blood ◽  
Human Placenta ◽  
Tobacco Consumption ◽  
Maternal Blood ◽  
Favourable Effect ◽  
Foetal Development ◽  
Polycyclic Aromatic

Placenta constitutes a vital organ of exchange between mother and foetus. In addition to this favourable effect for foetal development, placenta indirectly may allow trans fer of several maternal blood xenobiotics. Human placenta and umbilical cord blood are interesting models for investigating maternal environment and the metabolism, the bioactivation and the transfer of carcinogens such as polycyclic aromatic hydrocarbons. We used them to assess the effect of a woman's smoking on the foetus. Few studies cover this subject. In pregnant women who have continued to smoke, benzo[a]pyrene compound of cigar ette smoke is metabolically activated to diol-epoxide derivative: benzo[a]pyrene-trans-7,8-dihydrodiol-9,10- epoxide, ultimate carcinogen (BPDE-I). This derivative is covalently fixed on DNA and gives BPDE-I-DNA adducts. By a competitive immunoassay technique, we determined BDPE-I-DNA adducts in 20 samples of placenta and umbilical cord blood from women who smoked (n=15) and who did not (n=10). Tobacco consumption was checked by urinary cotinine determination. In the group of smokers levels of adducts were found in 13 specimens of placenta (from 10 to 60 finol/50 μg of DNA) and 12 umbilical cord blood (from 10 to 22.15 fmol/50 μg of DNA) samples. These results indicate that a mother's tobacco consumption is linked to the accumulation of BPDE-I-DNA adducts in the placenta, which are seen in smaller quantities in the umbilical cord blood, probably because of the metabolic capacity of the placenta and the transfer of B[a]P from the mother to the foetus.

Detection of smoking-related covalent DNA adducts in human placenta

Science ◽  
1986 ◽  
Vol 231 (4733) ◽  
pp. 54-57 ◽  
Author(s):  
R. Everson ◽  
E Randerath ◽  
R. Santella ◽  
R. Cefalo ◽  
T. Avitts ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Human Placenta ◽  
Detection Of Smoking

DNA adducts in human placenta in relation to tobacco smoke exposure and plasma antioxidant status

1997 ◽  
Vol 123 (3) ◽  
pp. 141-151 ◽  
Author(s):  
Helmut Daube ◽  
Gerhard Scherer ◽  
Kirsten Riedel ◽  
Thomas Ruppert ◽  
Anthony R. Tricker ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Tobacco Smoke ◽  
Human Placenta ◽  
Antioxidant Status ◽  
Smoke Exposure ◽  
Tobacco Smoke Exposure ◽  
Plasma Antioxidant

Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples

1991 ◽  
Vol 37 (4) ◽  
pp. 532-535 ◽  
Author(s):  
T Vo-Dinh ◽  
J P Alarie ◽  
R W Johnson ◽  
M J Sepaniak ◽  
R M Santella
Keyword(s):  
Dna Adducts ◽  
Human Placenta ◽  
Fiber Optic ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Mild Acid Hydrolysis ◽  
Base Pairs ◽  
Fiber Optic Technology ◽  
Antigen Antibody ◽  
Mild Acid

Abstract This paper presents the application of a fiber-optic antibody-based fluoroimmunosensor (FIS) for measuring DNA adducts of benzo[alpha]pyrene in human placenta samples. The FIS combines the excellent specificity of the antigen-antibody reaction, the high sensitivity of laser excitation, and the versatility of fiber-optic technology. The FIS was used to detect benzo[alpha]pyrene tetrol (BPT) after release from DNA by mild acid hydrolysis. These placenta samples contained one molecule of adduct per 10(7) base pairs. The limit of detection of the FIS for BPT is about 14 amol (14 x 10(-18) mol).

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