Quantitation of Carcinogen-DNA Adducts with Monoclonal Antibodies

2015 ◽  
pp. 63-75 ◽  
Author(s):  
Regina M. Santella ◽  
Francis Gasparo ◽  
Ling-Ling Hsieh
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Adducts

scholarly journals Development of Aflatoxin B1-Lysine Adduct Monoclonal Antibody for Human Exposure Studies

2001 ◽  
Vol 67 (6) ◽  
pp. 2712-2717 ◽  
Author(s):  
Jia-Sheng Wang ◽  
Salahaddin Abubaker ◽  
Xia He ◽  
Guiju Sun ◽  
Paul T. Strickland ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
High Risk ◽  
Bovine Serum Albumin ◽  
Dna Adducts ◽  
Human Exposure ◽  
Limit Of Detection ◽  
Serum Samples ◽  
Bovine Serum Albumin Conjugate ◽  
Aflatoxin B

ABSTRACT Mouse monoclonal antibodies were developed against a synthetic aflatoxin B1 (AFB)-lysine–cationized bovine serum albumin conjugate. The isotype of one of these antibodies, IIA4B3, has been classified as immunoglobulin G1(λ). The affinity and specificity of IIA4B3 were further characterized by a competitive radioimmunoassay. The affinities of IIA4B3 for AFB and its associated adducts and metabolites are ranked as follows: AFB-lysine > 8,9-dihydro-8-(2,6-diamino-4-oxo-3,4-dihydropyrimid-5-yl formamido)-9-hydroxy-AFB > AFB = 8,9-dihydro-8-(N 7-guanyl)-9-hydroxy-AFB > aflatoxin M1 > aflatoxin Q1. IIA4B3 had about a 10-fold higher affinity for binding to AFB-lysine adduct than to AFB when 3H-AFB–lysine was used as the tracer. The concentration for 50% inhibition for AFB-lysine was 0.610 pmol; that for AFB was 6.85 pmol. IIA4B3 had affinities at least sevenfold and twofold higher than those of 2B11, a previously developed antibody against parent AFB, for the major aflatoxin-DNA adducts 8,9-dihydro-8-(N 7-guanyl)-9-hydroxy-AFB and 8,9-dihydro-8-(2,6-diamino-4-oxo-3,4-dihydropyrimid-5-yl formamido)-9-hydroxy-AFB, respectively. An analytical method based on a competitive radioimmunoassay with IIA4B3 and3H-AFB–lysine was validated with a limit of detection of 10 fmol of AFB-lysine adduct. The method has been applied to the measurement of AFB-albumin adduct levels in human serum samples collected from the residents of areas at high risk for liver cancer.

Monoclonal antibodies and rabbit antisera recognizing 4-aminobiphenyl—DNA adducts and application to immunoaffinity chromatography

1992 ◽  
Vol 13 (6) ◽  
pp. 917-922 ◽  
Author(s):  
John D. Groopman ◽  
Paul L. Skipper ◽  
Paul R. Donahue ◽  
Laura J. Trudel ◽  
Michael Wildschutte ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Adducts ◽  
Immunoaffinity Chromatography

A Monofunctional Derivative of Melphalan:  Preparation, DNA Alkylation Products, and Determination of the Specificity of Monoclonal Antibodies That Recognize Melphalan−DNA Adducts

1998 ◽  
Vol 11 (10) ◽  
pp. 1162-1168 ◽  
Author(s):  
Michael J. Tilby ◽  
Hazel McCartney ◽  
Katherine A. Gould ◽  
C. Caroline O'Hare ◽  
John A. Hartley ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Adducts ◽  
Dna Alkylation

scholarly journals Detection of Acrolein-Derived Cyclic DNA Adducts in Human Cells by Monoclonal Antibodies

2012 ◽  
Vol 25 (12) ◽  
pp. 2788-2795 ◽  
Author(s):  
Jishen Pan ◽  
Bisola Awoyemi ◽  
Zhuoli Xuan ◽  
Priya Vohra ◽  
Hsiang-Tsui Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Adducts ◽  
Human Cells

Abstract 120: Detection of acrolein-derived cyclic DNA adducts in human cells by monoclonal antibodies.

2013 ◽  
Author(s):  
Jishen Pan ◽  
Bisola Awoyemi ◽  
Zhuoli Xuan ◽  
Priya Vohra ◽  
Hsiang-Tsui Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Adducts ◽  
Human Cells

In situ detection of acetylaminofluorene–DNA adducts in human cells using monoclonal antibodies

DNA Repair ◽  
2004 ◽  
Vol 3 (11) ◽  
pp. 1475-1482 ◽  
Author(s):  
Taka-aki Iwamoto ◽  
Nobuhiko Kobayashi ◽  
Kyoko Imoto ◽  
Aya Yamamoto ◽  
Yu Nakamura ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Adducts ◽  
Human Cells ◽  
In Situ Detection

The binding efficiency of polyclonal and monoclonal antibodies to DNA modified with benzo[a]pyrene diol epoxide is dependent on the level of modification. Implications for quantitation of benzo[a]pyrene-DNA adducts in vivo

1987 ◽  
Vol 8 (9) ◽  
pp. 1263-1269 ◽  
Author(s):  
Frederik Jan Van Schooten ◽  
Erik Kriek ◽  
Marie-José S.T. Steenwinkel ◽  
Hub P.J.M. Noteborn ◽  
Michel J.X. Hillebrand ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dna Adducts ◽  
Diol Epoxide ◽  
Antibodies To Dna
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