8 Isolation and Culture of Microvessel and Large-Vessel Endothelial Cells: Their Use in Transport and Clinical Studies

2015 ◽  
pp. 204-245 ◽  
Author(s):  
Stuart K. Williams
Keyword(s):  
Endothelial Cells ◽  
Clinical Studies ◽  
Large Vessel

scholarly journals Monocyte chemotactic factor produced by large vessel endothelial cells in vitro.

1986 ◽  
Vol 6 (3) ◽  
pp. 254-258 ◽  
Author(s):  
J A Berliner ◽  
M Territo ◽  
L Almada ◽  
A Carter ◽  
E Shafonsky ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Chemotactic Factor ◽  
Large Vessel

scholarly journals Critical Role for GATA3 in Mediating Tie2 Expression and Function in Large Vessel Endothelial Cells

2009 ◽  
Vol 284 (42) ◽  
pp. 29109-29124 ◽  
Author(s):  
Haihua Song ◽  
Jun-ichi Suehiro ◽  
Yasuharu Kanki ◽  
Yoshiko Kawai ◽  
Kenji Inoue ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Critical Role ◽  
Large Vessel ◽  
And Function

Stimulation of proteoglycans by IGF I and II in microvessel and large vessel endothelial cells

1987 ◽  
Vol 253 (1) ◽  
pp. E21-E27
Author(s):  
R. S. Bar ◽  
B. L. Dake ◽  
S. Stueck
Keyword(s):  
Endothelial Cells ◽  
Pulmonary Artery ◽  
Vascular Endothelial Cells ◽  
Pulmonary Arteries ◽  
Large Vessel ◽  
Dependent Manner ◽  
Disease States ◽  
Igf I ◽  
Stimulation Of

Endothelial cells were cultured from bovine capillaries and pulmonary arteries, and the effect of insulinlike growth factor (IGF) I and II (multiplication-stimulating activity) and insulin on the synthesis of proteoglycans was determined. IGF I and II stimulated 35SO4 incorporation into proteoglycans in a dose-dependent manner in both microvessel and pulmonary artery endothelial cells with maximum threefold increases. In pulmonary artery cells, the IGFs caused a general stimulation of all classes of glycosaminoglycan-containing proteoglycans. In microvessel endothelial cells, the IGFs appeared to preferentially increase heparan sulfate-containing proteoglycans. Insulin, at concentrations up to 10(-6) M, had no effect on the synthesis of proteoglycans in either microvessel or pulmonary arterial endothelial cells. Thus, the IGFs stimulate the synthesis of proteoglycans in both microvessel and large vessel endothelial cells, a property that is not mimicked by insulin. Because vascular endothelial cells are bathed by IGFs in vivo, such IGF-mediated functions are likely to be significant in both the normal physiology of vascular endothelium and in disease states such as diabetes mellitus.

A PRACTICAL QUESTION BASED ON CROSS-PLATFORM MICROARRAY DATA NORMALIZATION: ARE BOEC MORE LIKE LARGE VESSEL OR MICROVASCULAR ENDOTHELIAL CELLS OR NEITHER OF THEM?

2007 ◽  
Vol 05 (04) ◽  
pp. 875-893 ◽  
Author(s):  
AIXIANG JIANG ◽  
WEI PAN ◽  
LIMING C. MILBAUER ◽  
YU SHYR ◽  
ROBERT P. HEBBEL
Keyword(s):  
Endothelial Cells ◽  
Microarray Data ◽  
Gene List ◽  
Normalization Method ◽  
Large Vessel ◽  
Microvascular Endothelial Cells ◽  
Support Vector ◽  
Cross Platform ◽  
Microvascular Endothelial ◽  
Discriminative Gene

Since the available microarray data of BOEC (human blood outgrowth endothelial cells), large vessel, and microvascular endothelial cells were from two different platforms, a working cross-platform normalization method was needed to make these data comparable. With six HUVEC (human umbilical vein endothelial cells) samples hybridized on two-channel cDNA arrays and six HUVEC samples on Affymetrix arrays, 64 possible combinations of a three-step normalization procedure were investigated to search for the best normalization method, which was selected, based on two criteria measuring the extent to which expression profiles of biological samples of the same cell type arrayed on two platforms were indistinguishable. Next, three discriminative gene lists between the large vessel and the microvascular endothelial cells were achieved by SAM (significant analysis of microarrays), PAM (prediction analysis for microarrays), and a combination of SAM and PAM lists. The final discriminative gene list was selected by SVM (support vector machine). Based on this discriminative gene list, SVM classification analysis with best tuning parameters and 10,000 times of validations showed that BOEC were far from large vessel cells, they either formed their own class, or fell into the microvascular class. Based on all the common genes between the two platforms, SVM analysis further confirmed this conclusion.

scholarly journals Capturing Endothelial Cells by Coronary Stents - From Histology to Clinical Outcomes

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Miloje Tomasevic ◽  
Jelena Rakocevic ◽  
Milan Dobric ◽  
Srđan Aleksandric ◽  
Milica Labudovic
Keyword(s):  
Coronary Artery Disease ◽  
Endothelial Cells ◽  
Coronary Artery ◽  
Clinical Outcomes ◽  
Clinical Studies ◽  
Vascular Endothelial Cells ◽  
Late Complication ◽  
Promising Therapeutic Approach ◽  
Artery Disease ◽  
Stent Surface

Abstract Introduction of drug-eluting stents (DES) in the therapy of patients with coronary artery disease resulted in the significant reduction of in-stent restenosis compared to bare-metal stent (BMS) treatment. However, the high incidence of late stent thrombosis with DES emerged as one of the safety concerns after their implantation. Enhancing stent endothelization by improved early healing and neointimal strut coverage emerged as possible solution for this late complication. Endothelial progenitor cells (EPC) capturing stents are designed to promote in situ endothelization with immobilized, antihuman, anti-CD34 antibodies attached to the luminal stent surface. Anti-CD34 antibodies target and capture EPC from circulation, which further differentiate into vascular endothelial cells and form functional endothelial layer on the stent surface. These cells are also capable of secreting pro-angiogenic factors that stimulate local endothelial cells to proliferate and migrate. Preclinical and clinical studies proved feasibility, efficacy and safety of EPC capturing stents in stable and high-risk patients with coronary artery disease. Rapid and extensive endothelization of EPC capturing stents translated into favorable profile of clinical outcomes, comparable to efficacy of BMSs and DESs. Therefore, we here present the most important results from the experimental and clinical studies that explored ECP capturing strategy to enhance endothelization, reduce the incidence of instent thrombosis and improve outcomes of patients with coronary artery disease, along with the future perspectives in this promising therapeutic approach.

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