Growth Patterns of Nasopharyngeal Carcinoma Xenografts in Nude Mice

2015 ◽  
pp. 216-219
Author(s):  
Liu Taifu ◽  
Wang Jinghua ◽  
B. Tribukait ◽  
Cao Shilong
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Growth Patterns

scholarly journals Effects of different combined regimens of cisplatin, metformin, and quercetin on nasopharyngeal carcinoma cells and subcutaneous xenografts

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhongwei Chen ◽  
Zhen Zeng ◽  
Shanshan Zhu ◽  
Ying Zeng ◽  
Qihuang Lin ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Drug Combination ◽  
Drug Application ◽  
Inhibitory Effects ◽  
Single Drug ◽  
Combined Application ◽  
Subcutaneous Xenograft ◽  
Recurrent Nasopharyngeal Carcinoma ◽  
Combination Regimens

AbstractCisplatin, metformin, and quercetin are all reliable anticancer drugs. However, it is unclear how effective their different combination regimens are on the growth of nasopharyngeal carcinoma cell line Sune-1 and subcutaneous xenograft in nude mice. This study evaluated the effects of single-drug, two-drug, and three-drug simultaneous or sequential combined application of these drugs on the growth of Sune-1 cells and subcutaneous xenograft tumors in nude mice. The results showed that the different combination regimens of cisplatin, metformin and quercetin all had significant inhibitory effects on the proliferation of Sune-1 cells and the growth of subcutaneous xenografts in nude mice (P < 0.01), and the inhibition rate of the three drugs simultaneous combined application was significant Higher than the two-drug combination or single-drug application (P < 0.05), the contribution level of each drug in the three-drug combination application from high to low were cisplatin > metformin > quercetin. In summary, our results indicate that the simultaneous combination of cisplatin, metformin, and quercetin may synergistically inhibit the growth of Sune-1 cells and subcutaneous xenografts in nude mice through their different anticancer mechanisms, which may be clinically refractory and provide reference for chemotherapy of patients with recurrent nasopharyngeal carcinoma.

scholarly journals Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery

2022 ◽  
Vol 8 (1) ◽  
Author(s):  
Changyu Zhu ◽  
Xiaolei Jiang ◽  
Hua Xiao ◽  
Jianmei Guan
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Extracellular Vesicles ◽  
Nude Mice ◽  
Peripheral Blood ◽  
Cell Apoptosis ◽  
Expression Patterns ◽  
Carcinoma Cells ◽  
Luciferase Reporter ◽  
Relationship Of ◽  
Dual Luciferase Reporter Assay

AbstractRadioresistance prevails as one of the largest obstacles in the clinical treatment of nasopharyngeal carcinoma (NPC). Meanwhile, tumor-derived extracellular vesicles (TEVs) possess the ability to manipulate radioresistance in NPC. However, its mechanism remains to be further explored. Therefore, the current study set out to explore the mechanism of microRNA (miR)-142-5p delivered by TEVs in regard to the radiosensitivity of NPC. Firstly, peripheral blood samples were collected from patients with radioresistance and radiosensitivity, followed by RT-qPCR detection of miR-142-5p expression. A dual-luciferase reporter assay was carried out to elucidate the targeting relationship of miR-142-5p with HGF and EGF. In addition, radiotherapy-resistant NPC cell models were established by screening NPC cells with gradient increasing radiation exposure, and co-incubated with EVs isolated from miR-142-5p mimic-transfected NPC cells, followed by overexpression of HGF and EGF. Moreover, cell viability was detected by means of MTS, cell proliferation with a colony formation assay, cell apoptosis with flow cytometry, and expression patterns of related genes with the help of Western blot analysis. NPC xenotransplantation models in nude mice were also established by subcutaneous injection of 5-8FR cells to determine apoptosis, tumorigenicity, and radiosensitivity in nude mice. It was found that miR-142-5p was poorly expressed in peripheral blood from NPC patients with radioresistance. Mechanistic experimentation illustrated that miR-142-5p inversely targeted HGF and EGF to inactivate the HGF/c-Met and EGF/EGFR pathways, respectively. NPC cell apoptosis was observed to be augmented, while their radioresistance and proliferation were restricted by EVs-miR-142-5p or HGF silencing, or EGF silencing. Furthermore, EVs-miR-142-5p inhibited growth and radioresistance and accelerated the apoptosis of radiotherapy-resistant NPC cells in nude mice by inhibiting the HGF/c-Met and EGF/EGFR pathways. Collectively, our findings indicated that TEVs might inhibit the HGF/c-Met and EGF/EGFR pathways by delivering miR-142-5p into radiotherapy-resistant NPC cells to enhance radiosensitivity in NPC.

Early monitoring of radiotherapeutic effects of nasopharyngeal carcinoma xenografts in nude mice using 18F-FDG PET-CT imaging

2010 ◽  
Vol 29 (4) ◽  
pp. 374-378 ◽  
Author(s):  
Jian-Wei Yuan ◽  
Yan-Lin Fen ◽  
Wei-Jun Xian ◽  
Xiao-Hong He ◽  
Bai-Hong Yuan ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Fdg Pet ◽  
Ct Imaging ◽  
Pet Ct ◽  
18F Fdg ◽  
Fdg Pet Ct

Diffusion kurtosis imaging and tumour microstructure for monitoring response to radiotherapy in human nasopharyngeal carcinoma xenografts

2020 ◽  
Vol 50 (5) ◽  
pp. 548-555
Author(s):  
Xiang Zheng ◽  
Yunbin Chen ◽  
Dechun Zheng ◽  
Youping Xiao ◽  
Jiayou Chen ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Tumour Size ◽  
Diffusion Kurtosis Imaging ◽  
X Ray ◽  
Human Nasopharyngeal Carcinoma ◽  
Xenograft Models ◽  
D Values ◽  
Diffusion Kurtosis ◽  
Fractional Irradiation

Abstract Objective To investigate the correlations and feasibility of diffusion kurtosis imaging (DKI) parameters and tumour histopathology after radiotherapy in human nasopharyngeal carcinoma (NPC) xenografts on nude mice. Materials and Methods Seventy-two nude mice were used for the construction of CNE-1 (radio-insensitive) and CNE-2 (radio-sensitive) NPC xenograft models, followed by fraction irradiation at different doses of X-ray. The nude mice were randomly divided into six groups in each cell line models according to the dose of X-ray they have received and with six mice in each group. DKI scan was performed after radiation. DKI parameters, tumour histopathology and AQP-1 biomarkers were detected. One-way ANOVA and Pearson’s correlation analysis were used in statistical analysis. Results In CNE-1 and CNE-2 NPC xenografts, D values were increased (P &lt; 0.01 and P &lt; 0.001), while K values (P &lt; 0.01 and P &lt; 0.001) and tumour size (P &lt; 0.001 and P &lt; 0.001) were reduced during fraction irradiation. Additionally, cell density (CD) and AQP-1 expressions were decreased, and necrosis ratio (NR) was increased in CNE-2 xenografts after fraction irradiation (P &lt; 0.001). The changes in D values were negatively correlated with tumour size (r = −0.856, P &lt; 0.001), CD (r = −0.918, P &lt; 0.001), AQP-1 mRNA (r = −0.856, P &lt; 0.001) and protein (r = −0.381, P = 0.022) expressions while positively correlated with NR (r = 0.908, P &lt; 0.001) in CNE-2 xenografts. The changes in K values were positively correlated with tumour size (r = 0.964, P &lt; 0.001), CD (r = 0.888, P &lt; 0.001), AQP-1 mRNA (r = 0.955, P &lt; 0.001) and protein (r = 0.330, P = 0.049) expression levels while negatively correlated with NR (r = −0.930, P &lt; 0.001). However, in CNE-1 xenografts, there were no correlation between DKI parameters and the expression of AQP-1. Conclusion Changes in D and K parameters after fractional irradiation are closely related with NPC cellular and pathological characteristics, especially size reduction and necrosis induction. These parameters exhibit potential abilities of monitoring the response to fractional irradiation in radio-sensitive NPC xenografts.

scholarly journals microRNA-613 exerts anti-angiogenic effect on nasopharyngeal carcinoma cells through inactivating the AKT signaling pathway by down-regulating FN1

2019 ◽  
Vol 39 (7) ◽  
Author(s):  
Ru Gao ◽  
Qiaolei Feng ◽  
Guolin Tan
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Signaling Pathway ◽  
Nude Mice ◽  
B Cell Lymphoma ◽  
Underlying Mechanism ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Angiogenic Effect ◽  
Regulatory Effects ◽  
Unclear Etiology

Abstract Background: Nasopharyngeal carcinoma (NPC) is a disease highly sensitive to radiotherapy with the unclear etiology. However, the specific effects of microRNA-613 (miR-613) on NPC still remain elusive. Therefore, the present study probes into the underlying mechanism of miR-613 in NPC via AKT signaling pathway by regulating Fibronectin 1 (FN1). Methods: First, microarray analysis was used to screen differentially expressed genes (DEGs) and regulatory miRs associated with NPC. Next, miR-613 and FN1 expression in NPC cells was determined, followed by verification of target relationship between miR-613 and FN1. With NPC cells exposed to miR-613 mimic, si-FN1 and LY294002 (inhibitor of AKT signaling pathway), the regulatory effects of miR-613 on proliferation, apoptosis, invasion, migration and angiogenesis of NPC cells were detected with ratio of B-cell lymphoma 2/Bcl-2-associated X protein (Bcl-2/Bax), Cleaved-caspase3, matrix metallopeptidase 2 (MMP-2), MMP-9, vascular endothelial growth factor (VEGF), and cell adhesion molecule-1 (CD31) expression measured. Then, tumorigenesis and MVD were determined after Xenograft in nude mice. Results: FN1 modulated by miR-613 was critical for NPC via the AKT signaling pathway. NPC cells exhibited down-regulated miR-613 and up-regulated FN1. Besides, miR-613 was verified to target FN1. Moreover, overexpressed miR-613, silenced FN1 or LY294002 treatment suppressed proliferation, invasion, migration, and angiogenesis in NPC cells, which was indicated by reduced expression of AKT, mTOR, MMP-2, MMP-9, VEGF, and CD31 as well as decreased ratio of Bcl-2/Bax and increased expression of Cleaved-caspase3. Furthermore, cell apoptosis was promoted and tumorigenesis and MVD in nude mice were inhibited with overexpression of miR-613, silenced FN1 or LY294002 treatment. Conclusion: Taken together, miR-613 inhibits angiogenesis in NPC cells through inactivating FN1-dependent AKT signaling pathway.

scholarly journals Contrast-enhanced Ultrasound in evaluating of angiogenesis and tumor staging of nasopharyngeal carcinoma in nude mice

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0221638 ◽  
Author(s):  
ShouJun Liang ◽  
Yong Gao ◽  
YaoLi Liu ◽  
ChengCheng Qiu ◽  
YanHao Chen ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Tumor Staging ◽  
Contrast Enhanced Ultrasound ◽  
Contrast Enhanced

DNA flow cytometric analysis of human nasopharyngeal carcinoma in nude mice

1989 ◽  
Vol 16 (2) ◽  
pp. 343-345 ◽  
Author(s):  
Cao Shilong ◽  
Liu Taifu ◽  
Wang Zhenhua ◽  
Zhou Jue ◽  
Huang Kangmei ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Human Nasopharyngeal Carcinoma
Sign in / Sign up

Export Citation Format

Share Document