A Rationale for the Selection of Occlusion to Induce and Elicit Delayed Contact Hypersensitivity in the Guinea Pig

Author(s):  
E. V. Buehler
Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 767
Author(s):  
Fernando Benavides ◽  
Peter Sutovsky ◽  
Verónica López ◽  
Chelsey Kennedy ◽  
Luisa Echevarría

The guinea pig, as a livestock species, is still developing and growing throughout Peru and neighboring countries, as reflected by its increasing export since 2000. However, the selection of proven fertile males is tedious due to the absence of seminal parameter standards and the lack of safe semen collection techniques. Thus, pregnancy detection or live births are required for males’ selection. The purpose of this study was to describe the qualitative and quantitative semen parameters of fertile guinea pig males, to set reference values, and to validate a novel electroejaculation technique for the species. Semen was collected at weekly intervals from sixteen fertile males. Four transrectal electroejaculations were performed per male with 95% successful collections, yielding 39 viable semen samples. Seminal characteristics were as follows: pH 7.0 ± 0.13; ejaculate volume 0.67 ± 0.55 mL; sperm motility 90.81 ± 6.64%; sperm concentration 36.7 ± 28.41 × 106 sperm/mL; sperm count 20.09 ± 17.56 × 106 sperm/ejaculate; percentage of abnormal morphology 18.26 ± 8.52%; and percentage ubiquitinated spermatozoa 5.57 ± 6.28%. These values will serve as a reference to detect best breeding and infertile males rapidly. The described techniques are reproducible by commercial producers.


1994 ◽  
Vol 23 (2) ◽  
pp. 179-187
Author(s):  
D. A. EDWARDS ◽  
T. M. SORANNO ◽  
M. A. AMORUSO ◽  
R. V. HOUSE ◽  
A. C. TUMMEY ◽  
...  

1993 ◽  
Vol 28 (4) ◽  
pp. 235-242 ◽  
Author(s):  
Ryuichi Kashima ◽  
Yumiko Oyake ◽  
Joskin Okaua ◽  
Yuzo Ikeda

1982 ◽  
Vol 8 (4) ◽  
pp. 246-255 ◽  
Author(s):  
Shuichi Tsuchiya ◽  
Mitsuo Kondo ◽  
Kikuhiko Okamoto ◽  
Yoshio Takase

2012 ◽  
Vol 127 (2) ◽  
pp. 567-581 ◽  
Author(s):  
Jin Hyen Baek ◽  
Yipin Zhou ◽  
David R. Harris ◽  
Dominik J. Schaer ◽  
Andre F. Palmer ◽  
...  

1993 ◽  
Vol 29 (1) ◽  
pp. 26-32 ◽  
Author(s):  
Ryuichi Kashima ◽  
Yumiko Oyake ◽  
Joshini Okada ◽  
Yuzo Ikeda

1963 ◽  
Vol 158 (971) ◽  
pp. 253-260 ◽  

Anti- Salmonella pullorum and anti- Brucella abortus sera produced in hedgehog, rat, rabbit, guinea-pig and ox were fed to young suckling hedgehogs. Anti- Salmonella agglutinins are absorbed far more readily than anti- Brucella agglutinins, with the exception of those produced in the guinea-pig which have a comparable rate of entry via the gut to the circulation. Homologous anti- Salmonella agglutinins are absorbed more readily than those from other species. No detectable passage of agglutinin occurred from hedgehog anti- Brucella serum produced as a result of a single immunizing injection, whereas some passage occurred from hyperimmune hedgehog serum of similar titre. Thus the gut of the young hedgehog is capable of showing a selection between antibodies produced in the same species in response to the same antigen. The gut also exhibits selection between antibodies produced in the same species in response to different antigens. These facts, and the closely related information on the capacities of the gut of the young rat, are discussed in relation to the location of antibodies among the serum proteins and the species in which the antibodies were produced.


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