Removal, Preparation, and Storage of Human Plasma for Radioimmunological Detection of Prostaglandins*

2015 ◽  
pp. 62-67 ◽  
Author(s):  
H. Sinzinger ◽  
S. Reiter ◽  
B. A. Peskar
Keyword(s):  
Human Plasma ◽  
And Storage

Investigations on the Nature of Hemophilia A

1963 ◽  
Vol 09 (01) ◽  
pp. 030-052 ◽  
Author(s):  
Eberhard Mammen
Keyword(s):  
Factor Viii ◽  
Human Plasma ◽  
Barium Sulfate ◽  
Freeze Drying ◽  
Room Temperature ◽  
Hemophilia A ◽  
Normal Human Plasma ◽  
Normal Human ◽  
And Storage ◽  
Prothrombin Activation

SummaryIn this paper an inhibitor is described that is found in hemophilic plasma and serum different from any till now described inhibitor. The inhibitor only inhibits prothrombin activation in the “intrinsic clotting systems”. This inhibitor is probably not present in normal human plasma or serum. It is destroyed by ether and freeze drying, is labile to acid and storage at room temperature. It is stable upon dialysis and has not been adsorbed on barium sulfate, aluminum hydroxide or kaolin. It precipitates at 50% v/v saturation with alcohol. The nature of this inhibitor seems to be a protein or lipoprotein.Factor VIII was isolated from hemophilic plasma. The amount isolated was the same as from normal plasma and the activity properties were not different. Hemophiliacs have normal amounts of factor VIII.

Stability of ceftolozane in human plasma and dried blood spots: Implications for transport and storage

2020 ◽  
Vol 103 ◽  
pp. 106692
Author(s):  
Jens Martens-Lobenhoffer ◽  
Matthias Hinderhofer ◽  
Uwe Tröger ◽  
Stefanie M. Bode-Böger
Keyword(s):  
Human Plasma ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
And Storage

Insoluble human plasma protein immunoadsorbents. Large-scale production and storage

1977 ◽  
Vol 74 (3) ◽  
pp. 237-245 ◽  
Author(s):  
Samuel T. Nerenberg ◽  
Rameshwar Prasad ◽  
Nancy Biskup ◽  
Linda Pedersen (Demarco)
Keyword(s):  
Human Plasma ◽  
Plasma Protein ◽  
Large Scale ◽  
Scale Production ◽  
Human Plasma Protein ◽  
Large Scale Production ◽  
And Storage

scholarly journals Collection and Storage of Human Plasma for Measurement of Oxylipins

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 137
Author(s):  
Kristen J. Polinski ◽  
Michael Armstrong ◽  
Jonathan Manke ◽  
Jennifer Seifert ◽  
Tessa Crume ◽  
...  
Keyword(s):  
Human Plasma ◽  
Ethylenediaminetetraacetic Acid ◽  
Small Subset ◽  
Omega 3 ◽  
Sample Collection ◽  
Resolvin D1 ◽  
Heparin Plasma ◽  
Liquid Chromatography Tandem Mass ◽  
Processing And Storage ◽  
And Storage

Oxylipins derived from omega-3 and -6 fatty acids are actively involved in inflammatory and immune processes and play important roles in human disease. However, as the interest in oxylipins increases, questions remain regarding which molecules are detectable in plasma, the best methods of collecting samples, and if molecules are stable during collection and storage. We thereby built upon existing studies by examining the stability of an expanded panel of 90 oxylipins, including specialized pro-resolving lipid mediators (SPMs), in human plasma (n = 5 subjects) during sample collection, processing, and storage at −80 °C. Oxylipins were quantified using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Blood samples collected in ethylenediaminetetraacetic acid (EDTA) or heparin followed by up to 2 h at room temperature prior to processing showed no significant differences in oxylipin concentrations compared to immediately processed samples, including the SPMs lipoxin A4 and resolvin D1. The majority of molecules, including SPMs, remained stable following storage for up to 1 year. However, in support of previous findings, changes were seen in a small subset of oxylipins including 12-HETE, TXB2, 14-HDHA, and 18-HEPE. Overall, this study showed that accurate measurements of most oxylipins can be obtained from stored EDTA or heparin plasma samples using LC/MS/MS.

Lyophilized, Reconstituted Human Plasma-, A Better Substrate Than Purified Fibrinogen For Thrombin Activity Assay

1981 ◽  
Author(s):  
F Brosstad ◽  
H C Godal
Keyword(s):  
Human Plasma ◽  
Freeze Drying ◽  
Storage Stability ◽  
Precise Determination ◽  
Gel Point ◽  
Activity Assay ◽  
Clotting Time ◽  
Original Volume ◽  
And Storage

The clotting time of purified fibriiwgen/thrcinbin mixtures is regularly used for assay and standardization of thrcmbin activity. However, considerable batch-tobatch variations as well as difficulties in obtaining precise determination of the visual gel point are nharasteristics of purified fibrinogen preparations, making them suboptimal for these purposes. In contrast, thrombin (1 NIH U) clotting time of pooled, human citrated plasma varies only insignificantly from batch to batch,and the readability of the gel point and storage stability at -20°C are excellent . For a more general use of pooled plasma as substrate for thrcnibin assay a lyophilized specimen would obviously be more practicable. To study this, pooled citrated hunan plasma was either lyophilized directly, or subsequent to dialysis against 0.2M citrated buffer pH 7.4 and reconstituted to original volume with water after storage at either +20°C or -20°C for varying periods of time.Conclusion: Only plasma dialysed prior to freeze-drying shewed a pH and a thrombin clotting time identical to that of unprocessed plasma. Such lyophilized plasma can be kept at rocm temperature for at least 1 year (our present limit of observation) wihtout any sign of deterioration, and represent a premising alternative to presently used thrombin substrates.

Human plasma stability during handling and storage: impact on NMR metabolomics

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1168-1177 ◽  
Author(s):  
Joana Pinto ◽  
M. Rosário M. Domingues ◽  
Eulália Galhano ◽  
Cristina Pita ◽  
Maria do Céu Almeida ◽  
...  
Keyword(s):  
Human Plasma ◽  
Room Temperature ◽  
Storage Conditions ◽  
Plasma Composition ◽  
Long Term Storage ◽  
Short And Long Term ◽  
The Stability ◽  
And Storage ◽  
Term Storage

The stability of human plasma composition was investigated by NMR, considering different collection tubes, time at room temperature (RT), short- and long-term storage conditions and up to 5 consecutive freeze–thaw cycles.

scholarly journals Effects of anticoagulants and storage conditions on clinical oxylipid levels in human plasma

2018 ◽  
Vol 1863 (12) ◽  
pp. 1511-1522 ◽  
Author(s):  
Hulda S. Jonasdottir ◽  
Hilde Brouwers ◽  
René E.M. Toes ◽  
Andreea Ioan-Facsinay ◽  
Martin Giera
Keyword(s):  
Human Plasma ◽  
Storage Conditions ◽  
And Storage

scholarly journals Study of the Stability of Factor VIII Activity in Human Plasma for Fractionation When Modeling Deviations in the Storage and Transportation Temperature Conditions

2020 ◽  
Vol 20 (3) ◽  
pp. 202-207
Author(s):  
A. A. Gorodkov ◽  
A. L. Poptsov ◽  
A. L. Khokhryakov
Keyword(s):  
Factor Viii ◽  
Human Plasma ◽  
Temperature Regime ◽  
Factor Viii Activity ◽  
Clotting Factors ◽  
Short Term ◽  
Temperature Conditions ◽  
Plasma For Fractionation ◽  
The Stability ◽  
And Storage

The European Pharmacopoeia requires that the transportation and storage of human plasma for fractionation should be carried out at –20 °C or below, while allowing for some deviations in the temperature regime. The current Russian regulatory documentation requires the transportation and storage of plasma intended for the production of labile protein preparations (blood clotting factors) at –30 °C or lower. However, acceptable deviations from the temperature regime are not specified, which creates certain difficulties in their assessment by an authorised person during plasma batch release. The main tool in risk assessment is in-process control of factor VIII activity in plasma stored at inadequate temperature, which entails significant financial costs. The aim of the study was to assess stability of factor VIII activity in human plasma for fractionation when modeling deviations in the storage and transportation temperature regime and to assess the possibility of amending the regulatory documentation requirements. Materials and methods: only full individual doses of plasma obtained by apheresis were used in the experiments. The tests were performed under simulated high temperature conditions with accurate continuous recording of temperature by a measuring system. An automatic coagulation analyser was used to determine factor VIII activity. Quantitative evaluation of the results was carried out by comparing factor VIII activity in the plasma before freezing and in the tested plasma. Statistical processing of data was performed by descriptive statistics methods using Microsoft Excel 2007 applications. Results: no significant effect of short-term deviations in the storage temperature on the stability of factor VIII activity in human plasma for fractionation was observed. Conclusions: the obtained data can be used as a rationale for introducing changes in the official requirements for the storage and transportation temperature regime for human plasma for fractionation, as well as for including details of acceptable short-term deviations of the storage and transportation temperature regime in the regulatory documentation.

Optimal collection and storage conditions for catecholamine measurements in human plasma and urine

1993 ◽  
Vol 39 (12) ◽  
pp. 2503-2508 ◽  
Author(s):  
F Boomsma ◽  
G Alberts ◽  
L van Eijk ◽  
A J Man in 't Veld ◽  
M A Schalekamp
Keyword(s):  
Clinical Trials ◽  
Sample Preparation ◽  
Human Plasma ◽  
Storage Conditions ◽  
Urine Samples ◽  
Sodium Metabisulfite ◽  
Optimal Conditions ◽  
Plasma And Urine Samples ◽  
And Storage

Abstract Improvements in methodologies for measuring concentrations of catecholamines (CA) have led to an increasing use of these compounds as markers in the screening of patients and in long-term clinical trials. Because of the associated logistical problems, we have investigated the unresolved question of optimal conditions for sample preparation and for storage of plasma and urine samples. Results show that blood should be centrifuged within 1 h after collection; the use of a refrigerated centrifuge is not necessary. Once plasma is prepared, CA are stable for 1 day at 20 degrees C, 2 days at 4 degrees C, 1 month at -20 degrees C (or 6 months with added glutathione), and up to 1 year at -70 degrees C. CA are stable at 4 degrees C for 1 month in unpreserved urine and for 4 months in urine preserved with EDTA and sodium metabisulfite. In acidified urine, CA were nearly unchanged after 1 year at 4 and -20 degrees C.

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