27 Circadian Rhythm in Number of Erythroid and Granulocytic Colony Forming Units in Culture (ECFU-C and GCFU-C) in Bone Marrow of BDF1 Male Mice

2015 ◽  
pp. 160-164
Author(s):  
P Bartlett ◽  
E Haus ◽  
T Tuason ◽  
L Sackett-Lundeen ◽  
D Lakatua
Keyword(s):  
Bone Marrow ◽  
Circadian Rhythm ◽  
Male Mice ◽  
Colony Forming Units ◽  
Granulocytic Colony

Preliminary Study on the Anti-radiation Effect of Jen-Sheng-Yang-Yung-Tang

1993 ◽  
Vol 21 (02) ◽  
pp. 187-195 ◽  
Author(s):  
Hsue-yin Hsu ◽  
Yau-hui Ho ◽  
Shi-Iong Lian ◽  
Chun-ching Lin
Keyword(s):  
Bone Marrow ◽  
Radiation Effect ◽  
Bone Marrow Cells ◽  
X Rays ◽  
Male Mice ◽  
X Ray ◽  
Colony Forming Units ◽  
Before And After ◽  
Preliminary Study ◽  
Different Doses

Six to seven week old male mice of ICR strain were exposed to different doses of x-rays to determine if Jen-Sheng-Yang-Yung-Tang could be a modifier in the elimination of radiation damage. Colony forming units of bone marrow cells in the spleen (CFUs) were measured before and after x-ray irradiation with intraperitoneal injection of 10 mg/20 g or 20 mg/20 g body weight of Jen-Sheng-Yang-Yung-Tang, once a day for seven consecutive days. The recovery of CFUs and hemocytes counts by 4 Gy irradiation with Jen-Sheng-Yang-Yung-Tang administration was faster for a concentration of 20 mg/20 g than 10 mg/20 g. The measurement of 10-day CFUs showed an increase of radiotolerance in the treatment of 20 mg/20 g administration before x-ray irradiation. The injection of Jen-Sheng-Yang-Yung-Tang accelerated the recovery of hemocyte counts in mice irradiated with 4 Gy x-ray; the effect was especially profound for leukocytes with 20 mg/20 g Jen-Sheng-Yang-Yung-Tang administration after irradiation.

scholarly journals Effect of aqueous extract of Rosemary officinalis on cytotoxicity of CCL4 induced albino male mice

2018 ◽  
Vol 12 (1) ◽  
pp. 124-131
Author(s):  
Ruqaya M. Ibrahim
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Aqueous Extract ◽  
Bone Marrow Cells ◽  
Post Treatment ◽  
Rosemary Extract ◽  
Mutagenic Action ◽  
Male Mice ◽  
Micronucleus Formation ◽  
Marrow Cells

This study focused the line on the effect of aqueous extract of Rosemary officinalis, as well as, effect of toxic compound CCL4, on micronucleus formation and mitotic index assay in albino male mice. This work started at September 2017 at Biotechnology Research center \Al-Nahrain University, by using 20 albino male mice. The result indicated that aqueous extract of rosemary caused significant increased in mitotic index and decrease micronucleus formation for two doses tested 50,100 mg/kg in comparison with negative and positive controls, also the results revealed that CCL4 showed significant mutagenic action on biological system of treated mice by increased frequency of micronucleus formation and decreased the percentage of mitotic index in bone marrow cells. Pre-and post –treatment between aqueous extract and CCL4 were also made. The results of pre and post treatment with rosemary extract were also caused a significant decreased in micronucleus formation and increase the percentage of mitotic index for two doses 50,100 mg/kg  in comparison with its corresponding controls which caused increased in the frequencies of micronucleus formation and decrease the percentage of mitotic index in bone marrow cells. Conclusions: Rosemary officinalis enhanced immunity, reduced mutagenic effects against cytotoxicity of CCL4.

scholarly journals Major Histocompatibility Complex Restriction Between Hematopoietic Stem Cells and Stromal Cells In Vivo

Blood ◽  
1997 ◽  
Vol 89 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Futoshi Hashimoto ◽  
Kikuya Sugiura ◽  
Kyoichi Inoue ◽  
Susumu Ikehara
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Major Histocompatibility Complex ◽  
Hematopoietic Stem Cells ◽  
Stromal Cells ◽  
Graft Failure ◽  
Major Histocompatibility ◽  
Hematopoietic Stem ◽  
Histocompatibility Complex ◽  
Colony Forming Units

Graft failure is a mortal complication in allogeneic bone marrow transplantation (BMT); T cells and natural killer cells are responsible for graft rejection. However, we have recently demonstrated that the recruitment of donor-derived stromal cells prevents graft failure in allogeneic BMT. This finding prompted us to examine whether a major histocompatibility complex (MHC) restriction exists between hematopoietic stem cells (HSCs) and stromal cells. We transplanted bone marrow cells (BMCs) and bones obtained from various mouse strains and analyzed the cells that accumulated in the engrafted bones. Statistically significant cell accumulation was found in the engrafted bone, which had the same H-2 phenotype as that of the BMCs, whereas only few cells were detected in the engrafted bones of the third-party H-2 phenotypes during the 4 to 6 weeks after BMT. Moreover, the BMCs obtained from the MHC-compatible bone showed significant numbers of both colony-forming units in culture (CFU-C) and spleen colony-forming units (CFU-S). These findings strongly suggest that an MHC restriction exists between HSCs and stromal cells.

scholarly journals Hematopoietic Recovery after Transplantation CD117+B220- (lacZ+) Bone Marrow Cells in Lethally Irradiated Mice

2008 ◽  
Vol 51 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Miroslav Hodek ◽  
Jiřina Vávrová ◽  
Zuzana Šinkorová ◽  
Jaroslav Mokrý ◽  
Stanislav Filip
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Repair Process ◽  
Whole Body ◽  
Lacz Gene ◽  
Colony Forming Units ◽  
The Difference ◽  
Macrophage Colony ◽  
Marrow Cells

Experiments presented here were aimed at the description of hematopoiesis repair and in vivo homing of transplanted separated CD117+B220–bone marrow cells after whole-body lethal irradiation at LD 9Gy. ROSA 26 mice were used as donors of marrow cells for transplantation [B6;129S/Gt (ROSA)26Sor] and were tagged with lacZ gene, and F2 hybrid mice [B6129SF2/J] were used as recipients of bone marrow transplanted cells. Hematopoiesis repair was provided by transplantation, both suspension of whole bone marrow cells (5x106) and isolated CD117+B220–cells (5x104). Mice survived up to thirty days after irradiation. We demonstrated that transplantation of suspension of whole bone marrow cells led to faster recovery of CFU-GM (Granulocyte-macrophage colony forming units) in bone marrow and in the spleen too. It is not clear what the share of residential and transplanted cells is in the repair process. Our results demonstrate that sufficient hematopoietic repair occurs after transplantation of CD117+B220–(lacZ+) in lethally irradiated mice, and the difference in CFU-GM numbers in the bone marrow and spleen found on day 8 posttransplant has no influence on the survival of lethally irradiated mice (30 days follow-up).

scholarly journals Chronic myelodysplastic syndrome (preleukemia) with the Philadelphia chromosome

Blood ◽  
1980 ◽  
Vol 56 (2) ◽  
pp. 262-264 ◽  
Author(s):  
DG Roth ◽  
CM Richman ◽  
JD Rowley
Keyword(s):  
Bone Marrow ◽  
Myelodysplastic Syndrome ◽  
Bone Marrow Cells ◽  
Philadelphia Chromosome ◽  
Laboratory Findings ◽  
Chronic Granulocytic Leukemia ◽  
Hematologic Disorder ◽  
Erythroid Hyperplasia ◽  
Granulocytic Colony

Abstract A patient with severe anemia, reticulocytopenia, and erythroid hyperplasia of the bone marrow developed fatal acute nonlymphocytic leukemia after 3 yr. A Philadelphia chromosome with the typical 9/22 translocation t(9q +;22q-) was identified by banding techniques in a small number of bone marrow cells throughout the preleukemic phase of the illness (14%--38% of metaphases) and during the acute transformation (50%). Granulocytic colony formation in vitro was abnormal in the preleukemic phase. The diagnosis of chronic granulocytic leukemia was excluded on the basis of clinical and laboratory findings. The identification of the Ph1 chromosome in this form of chronic myelodysplastic syndrome (preleukemia) provides a new example of a hematologic disorder predisposing to acute leukemia in which this chromosomal abnormality occurs.

Microwave Irradiation and the Circadian Rhythm of Bone Marrow Cell Mitosis*

1974 ◽  
Vol 9 (1) ◽  
pp. 31-37 ◽  
Author(s):  
P. Czerski ◽  
Ewa Paprocka-Słonka ◽  
Anna Stolarska
Keyword(s):  
Bone Marrow ◽  
Circadian Rhythm ◽  
Microwave Irradiation ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Cell Mitosis
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