Intracellular and Plasma Branched Chain Amino Acid Interrelationship with Special Reference to Human Muscle Tissue

2015 ◽  
pp. 268-278
Author(s):  
P. F�rst
Keyword(s):  
Amino Acid ◽  
Special Reference ◽  
Muscle Tissue ◽  
Human Muscle ◽  
Branched Chain Amino Acid ◽  
Branched Chain

Effects of amino acids on Thiobacillus acidophilus I. Growth studies with special reference to valine

1978 ◽  
Vol 24 (10) ◽  
pp. 1158-1163
Author(s):  
Gérald Proteau ◽  
Marvin Silver
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Special Reference ◽  
Branched Chain Amino Acids ◽  
Heterotrophic Growth ◽  
Branched Chain Amino Acid ◽  
Crude Extracts ◽  
Optimum Ph ◽  
Thiobacillus Acidophilus ◽  
Branched Chain

The heterotrophic growth of Thiobacillus acidophilus was inhibited by branched-chain amino acids; valine, isoleucine, and leucine. The inhibition by valine and leucine were partially reversed by isoleucine, and the inhibition by isoleucine was partially reversed by valine. Inhibitions by methionine or threonine were partially reversed when both amino acids were present in the growth medium. Inhibition by tyrosine was increased by phenylalanine or tryptophan. Cystine completely inhibited growth. Other amino acids tested produced little or no inhibition.Acetohydroxy acid synthetase (AHAS) activity was demonstrated in crude extracts of T. acidophilus. In crude extracts the optimum pH was 8.5 with a shift to 9.0 in the presence of valine. Valine was the only branched-chain amino acid which inhibited the AHAS activity. The presence of only one peak of AHAS activity upon centrifugation in linear glycerol density gradients demonstrated that the AHAS activity sediments as one component.

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