Genetics of Exopolysaccharide Production by Mucoid Pseudomonas aeruginosa

2015 ◽  
pp. 13-22 ◽  
Author(s):  
Dennis E. Ohman ◽  
Joanna B. Goldberg ◽  
Jo Anne L. Flynn ◽  
Sharon K. Powell
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Exopolysaccharide Production

scholarly journals Composition of Alginate Synthesized during the Growth Cycle of Pseudomonas aeruginosa

1987 ◽  
Vol 40 (4) ◽  
pp. 435 ◽  
Author(s):  
Geoffrey Annison ◽  
lain Couperwhite
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Magnetic Resonance ◽  
Cystic Fibrosis Patient ◽  
Batch Culture ◽  
Mole Fraction ◽  
Proton Magnetic Resonance ◽  
Growth Cycle ◽  
Exponential Phase ◽  
Exopolysaccharide Production

A mucoid P. aeruginosa isolated from the sputum of a cystic fibrosis patient was grown in batch culture on a complex medium. During the growth cycle the amount of alginate produced was estimated and its composition was determined by proton magnetic resonance (IH-n.m.r) spectroscopy. Exopolysaccharide production occurred mainly during the exponential phase of growth. The alginate samples isolated varied little in composition and were characterized by being highly acetylated, high mannuronate (0.83 - 0.93 mole fraction) polymers. Guluronate was present only within heteropolymeric regions of the polysaccharides which all displayed a complete absence of polyguluronate. Ca2 + ion supplementation of the medium was not observed to increase the levels of guluronate in the algi nates produced.

scholarly journals Regulation of Exopolysaccharide Production by ProE, a Cyclic-Di-GMP Phosphodiesterase in Pseudomonas aeruginosa PAO1

2020 ◽  
Vol 11 ◽  
Author(s):  
Qishun Feng ◽  
Stephen Dela Ahator ◽  
Tian Zhou ◽  
Zhiqing Liu ◽  
Qiqi Lin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Exopolysaccharide Production ◽  
Pseudomonas Aeruginosa Pao1

scholarly journals Coordination of Swarming Motility, Biosurfactant Synthesis, and Biofilm Matrix Exopolysaccharide Production in Pseudomonas aeruginosa

2014 ◽  
Vol 80 (21) ◽  
pp. 6724-6732 ◽  
Author(s):  
Shiwei Wang ◽  
Shan Yu ◽  
Zhenyin Zhang ◽  
Qing Wei ◽  
Lu Yan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Bacterial Survival ◽  
Swarming Motility ◽  
Content Type ◽  
Exopolysaccharide Production ◽  
Complex Process ◽  
Exopolysaccharide Biosynthesis ◽  
Biofilm Matrix

ABSTRACTBiofilm formation is a complex process in which many factors are involved. Bacterial swarming motility and exopolysaccharides both contribute to biofilm formation, yet it is unclear how bacteria coordinate swarming motility and exopolysaccharide production. Psl and Pel are two key biofilm matrix exopolysaccharides inPseudomonas aeruginosa. This opportunistic pathogen has three types of motility, swimming, twitching, and swarming. In this study, we found that elevated Psl and/or Pel production reduced the swarming motility ofP. aeruginosabut had little effect on swimming and twitching. The reduction was due to decreased rhamnolipid production with no relation to the transcription ofrhlAB, two key genes involved in the biosynthesis of rhamnolipids. Rhamnolipid-negativerhlRandrhlABmutants synthesized more Psl, whereas exopolysaccharide-deficient strains exhibited a hyperswarming phenotype. These results suggest that competition for common sugar precursors catalyzed by AlgC could be a tactic forP. aeruginosato balance the synthesis of exopolysaccharides and rhamnolipids and to control bacterial motility and biofilm formation inversely because the biosynthesis of rhamnolipids, Psl, and Pel requires AlgC to provide the sugar precursors and an additionalalgCgene enhances the biosynthesis of Psl and rhamnolipids. In addition, our data indicate that the increase in RhlI/RhlR expression attenuated Psl production. This implied that the quorum-sensing signals could regulate exopolysaccharide biosynthesis indirectly in bacterial communities. In summary, this study represents a mechanism that bacteria utilize to coordinate swarming motility, biosurfactant synthesis, and biofilm matrix exopolysaccharide production, which is critical for biofilm formation and bacterial survival in the environment.

Sign in / Sign up

Export Citation Format

Share Document