Urinary Enzyme Measurements as Sensitive Indicators of Chronic Cadmium Nephrotoxicity

2015 ◽  
pp. 142-147 ◽  
Author(s):  
E. Bomhard ◽  
D. Maruhn ◽  
D. Paar ◽  
K. Wehling
Keyword(s):  
Urinary Enzyme

The diagnostic value of urinary enzyme measurements in hypertension

1983 ◽  
Vol 133 (3) ◽  
pp. 317-325 ◽  
Author(s):  
Ian D.A. Johnston ◽  
Norman F. Jones ◽  
John E. Scoble ◽  
Chun-Ting Yuen ◽  
Robert G. Price
Keyword(s):  
Diagnostic Value ◽  
Urinary Enzyme

ELECTROPHORETIC EVIDENCE FOR ISOZYMES OF ARYLAMIDASE ("AMINOPEPTIDASE") IN THE RAT. I. RENAL, SERUM AND URINARY ENZYMES SPLITTING dl-ALANYL-2-NAPHTHYLAMIDE AND l-LEUCYL-2-NAPHTHYLAMIDE

1964 ◽  
Vol 12 (12) ◽  
pp. 869-874 ◽  
Author(s):  
BENITO MONIS
Keyword(s):  
Present Report ◽  
Molecular Forms ◽  
Supernatant Fraction ◽  
Blood Levels ◽  
Rat Tissues ◽  
Bilateral Nephrectomy ◽  
Rat Urine ◽  
Urinary Enzyme ◽  
Renal Ablation ◽  
Starting Point

Bilateral nephrectomy elicited no changes of serum blood levels of arylamidase assayed with two chromogenic substrates (l-leucyl-2-naphthylamide and dl-alanyl-2-naphthylamide) two days after the operation. Since rat urine contains similar enzymes it was postulated that the urinary enzyme is of renal origin and distinct from serum arylamidase. For this purpose, electrophoretic studies were undertaken. Starch block (for quantitative determinations) and starch gel for zymograms of arylamidase were used. It was shown that both procedures demonstrated the identity of urinary and renal arylamidase, which was distinct from the serum enzyme. The renal and urinary enzyme showed two distinct isozymes: one remaining at the origin and the other migrating somewhat less than the isozyme in serum. It is postulated that the isozyme remaining at the origin corresponds to a membrane-bound form, whereas the electrophoretically-mobile isozyme is found in the cellular supernatant fraction. The arylamidase from serum was represented by a single enzyme which migrated farthest towards the anode. Histochemical procedures for the demonstration of arylamidase activity in tissues at the light microscopic level permit the localization of enzyme(s) that can hydrolyze synthetic chromogenic naphthylamides containing l-leucyl and dl-alanyl groups (1). Rat kidneys have a high concentration of histochemically demonstrable arylamidase in the proximal convoluted tubules (2). Blood levels of arylamidase in the normal adult rat vary within a narrow range (3). The observation of arylamidase activity in rat urine raised several questions that led to the studies which are the basis of the present report. The relationship of serum and urinary arylamidase was the starting point of this investigation. It was speculated that if the urinary enzyme had its origin in the serum, bilateral nephrectomy should alter blood levels of the enzyme. If no such changes occurred, this would suggest that the urinary enzyme was released from the proximal convoluted tubule. In fact, total renal ablation led to no significant variation of serum arylamidase activity. It was therefore postulated that urinary arylamidase originated in the kidneys and that both are distinct from serum arylamidase. To test this hypothesis, zone electrophoretic studies were undertaken using two different supporting media. It will be shown that distinct molecular forms or isozymes of arylamidase can be separated from rat tissues and fluids by differential electrophoretic mobility. The electrophoretic identity of urinary and renal arylamidase, both of which are distinct from the serum arylamidase, is demonstrated in this study.

Effect of Amikacin and Gentamicin on Urinary Enzyme Excretion

1983 ◽  
Vol 17 (10) ◽  
pp. 752-753
Author(s):  
Juan Jimenez-Alonso ◽  
Luciano Barrios ◽  
Rafael Bejarano ◽  
Laura Jaimez ◽  
Francisco Perez-Jimenez ◽  
...  
Keyword(s):  
Urinary Enzyme ◽  
Urinary Enzyme Excretion

Urinary enzyme excretion by renal-transplant recipients in relation to interval after transplantation.

1982 ◽  
Vol 28 (8) ◽  
pp. 1762-1764 ◽  
Author(s):  
K Jung ◽  
J Diego ◽  
D Scholz ◽  
K Schröder ◽  
V Strobelt
Keyword(s):  
Renal Transplant ◽  
Reference Group ◽  
Transplant Rejection ◽  
Early Period ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Renal Disorder ◽  
Urinary Enzyme ◽  
Daily Dose ◽  
Urinary Enzyme Excretion

Abstract We determined the urinary excretion of the enzymes aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1), gamma-glutamyltransferase (EC 2.3.2.2), and N-acetyl-beta-glucosaminidase (EC 3.2.1.30) in two groups of renal-transplant recipients at different times after transplantation (1.8 months and 52 months, respectively). Both groups of patients showed a higher rate of enzyme excretion than did a reference group of healthy persons. More aminopeptidase and N-acetyl-beta-glucosaminidase were excreted during the early period after transplantation than later. The time-dependence of urinary enzyme excretion was confirmed in six renal-transplant recipients studied during the course of 15 months after transplantation. There was a general correlation between the extent of urinary enzyme excretion and both the time after transplantation and the daily dose of prednisolone. Therefore, it is necessary to take into account this influence on the extent of urinary enzyme in renal-transplant recipients if urinary enzyme excretion is used as an indicator of renal disorder and especially as an early predictor of transplant rejection.

Urinary enzyme measurements as sensitive indicators of nephrotoxicity in chronic toxicological studies

1983 ◽  
Vol 18 ◽  
pp. 36
Author(s):  
E. Bombard ◽  
D. Naruhn ◽  
D. Paar ◽  
K. Wehling
Keyword(s):  
Urinary Enzyme ◽  
Toxicological Studies
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