Early Receptor Potential in Rod and in Cone Dominated Eyes

2015 ◽  
pp. 59-67
Author(s):  
J. Tanabe ◽  
E. R. Lapp
Keyword(s):  
Receptor Potential ◽  
Early Receptor Potential

scholarly journals Electrophysiological measurement of the number of rhodopsin molecules in single Limulus photoreceptors.

1977 ◽  
Vol 70 (5) ◽  
pp. 621-633 ◽  
Author(s):  
J E Lisman ◽  
H Bering
Keyword(s):  
Relative Intensity ◽  
Surface Density ◽  
Receptor Potential ◽  
Absolute Intensity ◽  
Charge Movement ◽  
Half Time ◽  
Quantal Response ◽  
Early Receptor Potential ◽  
Electrophysiological Measurement ◽  
Electrophysiological Methods

Two partly independent electrophysiological methods are described for measuring the number of rhodopsin molecules (R) in single ventral photoreceptors. Method 1 is based on measurements of the relative intensity required to elicit a quantal response and the relative intensity required to half-saturate the early receptor potential (ERP). Method 2 is based on measurements of the absolute intensity required to elicit a quantal response. Both methods give values of R approximately equal to 10(9). From these and other measurements, estimates are derived for the surface density of rhodopsin (8,000/micrometer2), the charge movement during the ERP per isomerized rhodopsin (20 X 10(-21) C), and the half-time for thermal isomerization of rhodopsin (36yr).

scholarly journals Enigma of early receptor potential in fly eyes

1989 ◽  
Vol 29 (12) ◽  
pp. 1663-1670 ◽  
Author(s):  
S. Gagné ◽  
J.G.H Roebroek ◽  
D.G. Stavenga
Keyword(s):  
Receptor Potential ◽  
Early Receptor Potential

The early receptor potential (ERP)

1987 ◽  
Vol 66 (1) ◽  
pp. 35-74 ◽  
Author(s):  
Winfried M�ller ◽  
Helmut T�pke
Keyword(s):  
Receptor Potential ◽  
Early Receptor Potential

scholarly journals Adaptation in the Ventral Eye of Limulus is Functionally Independent of the Photochemical Cycle, Membrane Potential, and Membrane Resistance

1973 ◽  
Vol 61 (3) ◽  
pp. 273-289 ◽  
Author(s):  
A. Fein ◽  
R. D. DeVoe
Keyword(s):  
Membrane Potential ◽  
Time Constant ◽  
Dark Adaptation ◽  
Light Adaptation ◽  
Receptor Potential ◽  
Membrane Resistance ◽  
Initial Time ◽  
Early Receptor Potential ◽  
Time Courses ◽  
Sensitivity Changes

The early receptor potential (ERP), membrane potential, membrane resistance, and sensitivity were measured during light and/or dark adaptation in the ventral eye of Limulus. After a bright flash, the ERP amplitude recovered with a time constant of 100 ms, whereas the sensitivity recovered with an initial time constant of 20 s. When a strong adapting light was turned off, the recovery of membrane potential and of membrane resistance had time-courses similar to each other, and both recovered more rapidly than the sensitivity. The receptor depolarization was compared during dark adaptation after strong illumination and during light adaptation with weaker illumination; at equal sensitivities the cell was more depolarized during light adaptation than during dark adaptation. Finally, the waveforms of responses to flashes were compared during dark adaptation after strong illumination and during light adaptation with weaker illumination. At equal sensitivities (equal amplitude responses for identical flashes), the responses during light adaptation had faster time-courses than the responses during dark adaptation. Thus neither the photochemical cycle nor the membrane potential nor the membrane resistance is related to sensitivity changes during dark adaptation in the photoreceptors of the ventral eye. By elimination, these results imply that there are (unknown) intermediate process(es) responsible for adaptation interposed between the photochemical cycle and the electrical properties of the photoreceptor.

scholarly journals Properties of the pH-sensitive site that controls the lambda max of Limulus metarhodopsin.

1981 ◽  
Vol 77 (2) ◽  
pp. 191-203 ◽  
Author(s):  
J E Lisman ◽  
S Schulman ◽  
Y Sheline ◽  
P K Brown
Keyword(s):  
Intracellular Ph ◽  
Receptor Potential ◽  
Ph Sensitive ◽  
Phenol Red ◽  
Intracellular Recordings ◽  
Ph Buffering ◽  
Intracellular Injection ◽  
Early Receptor Potential ◽  
Sensitive Site ◽  
Secondary Result

A pH-sensitive site controls the lambda max of Limulus metarhodopsin. The properties of this site were examined using intracellular recordings of the early receptor potential (ERP) as a pigment assay. ERPs recorded over a range of extracellular pHs indicate that the apparent pK of the site is in the range of 8.3-8.6. Several lines of evidence indicate that the site responds directly to changes in extracellular pH (pHo) rather than to changes in intracellular pH(pHi) that follow as a secondary result of changing pHo : (a) the effect of changing pHo was rapid (less than 60 s); (b) when pHo was raised, the simultaneous rise in pHi, as measured with phenol red, was relatively small; (c) raising pHi by intracellular injection of pH 10 glycine buffer did not affect the site; and (d) the effect of changing pH0 could not be blocked by increasing the intracellular pH buffering capacity. It is concluded that the pH-sensitive site on metarhodopsin is on the extracellular surface of the plasma membrane.

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