Inhibition of Thickening of Glomerular Capillary Basement Membrane in Spontaneously Diabetic Rats

2015 ◽  
pp. 433-444
Author(s):  
Yoshio Goto ◽  
Masaei Kakizaki ◽  
Takayoshi Toyota ◽  
Shin-ichi Oikawa ◽  
Noboru Oikawa ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Diabetic Rats ◽  
Capillary Basement Membrane ◽  
Glomerular Capillary ◽  
Spontaneously Diabetic Rats

scholarly journals ELECTRON MICROSCOPY OF THE AVIAN RENAL GLOMERULUS

1957 ◽  
Vol 3 (2) ◽  
pp. 183-192 ◽  
Author(s):  
R. K. F. Pak Poy ◽  
J. S. Robertson
Keyword(s):  
Electron Microscopy ◽  
Basement Membrane ◽  
Thin Sheet ◽  
Cell Mass ◽  
Central Cell ◽  
Dense Layer ◽  
Central Mass ◽  
Capillary Basement Membrane ◽  
Glomerular Capillary ◽  
Glomerular Capillaries

Electron microscopy of sections of chicken glomeruli shows them to possess a large central cell mass, occupying the hilum and the centre of the glomerulus, and continuous with the adventitia of the afferent and efferent arterioles. The glomerular capillaries form a much simpler system than in mammals and are spread over the surface of the central cell mass. Between the capillaries the mass is limited externally by the major component of the glomerular capillary basement membrane, which continues over the surface of the mass from one capillary to the next. Projections of the central cell mass characteristically form the support for glomerular capillaries, and smaller knobs of the central mass may project actually into the lumen of the capillaries, but always carry a layer of endothelial cytoplasm before them. They are never in direct contact with blood. The basement membrane of the glomerular capillary loop has a central dense layer and two lateral less dense layers as in mammals. The central dense layer is continuous with similar appearing dense material in the intercellular spaces of the adventitiae of the arterioles, and also with that of the central cell mass. The two less dense layers can also be traced into direct continuity with the less dense regions of this intercellular substance. The endothelial cytoplasm is spread as a thin sheet over the inner surface of the capillary basement membrane, and shows scattered "pores" resembling those described in mammals. Epithelial cells with interlacing pedicels are at least as prominent as those in mammals. Bowman's capsular membrane also possesses three layers similar to but less wide than those of the capillary basement membrane, and all three layers can be traced into continuity with the dark and light regions of the intercellular material of the adventitial cells of the arterioles, and beyond them with that of the central cell mass. At the hilum Bowman's capsular membrane also fuses with the capillary basement membrane.

The KKAy Mouse: A Model for the Rapid Development of Glomerular Capillary Basement Membrane Thickening

1987 ◽  
Vol 24 (6) ◽  
pp. 297-303 ◽  
Author(s):  
Arthur R. Diani ◽  
Gerianne A. Sawada ◽  
Neng Y. Zhang ◽  
Beatrice M. Wyse ◽  
Christina L. Connell ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Rapid Development ◽  
Capillary Basement Membrane ◽  
Glomerular Capillary ◽  
Basement Membrane Thickening

scholarly journals Lack of Nitric Oxide Results in Increased Glomerular Capillary Basement Membrane Thickness

2009 ◽  
Vol 15 (S2) ◽  
pp. 852-853
Author(s):  
A Ellis ◽  
M Grant
Keyword(s):  
Nitric Oxide ◽  
Basement Membrane ◽  
Membrane Thickness ◽  
Basement Membrane Thickness ◽  
Capillary Basement Membrane ◽  
Glomerular Capillary

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

scholarly journals Basement membrane-specific chondroitin sulfate proteoglycan is abnormally associated with the glomerular capillary basement membrane of diabetic rats.

1994 ◽  
Vol 42 (4) ◽  
pp. 473-484 ◽  
Author(s):  
K J McCarthy ◽  
D R Abrahamson ◽  
K R Bynum ◽  
P L St John ◽  
J R Couchman
Keyword(s):  
Diabetes Mellitus ◽  
Basement Membrane ◽  
Chondroitin Sulfate ◽  
Matrix Protein ◽  
Core Protein ◽  
Chondroitin Sulfate Proteoglycan ◽  
Extracellular Matrix Protein ◽  
Sulfate Proteoglycan ◽  
Capillary Basement Membrane ◽  
Glomerular Capillary

We have previously reported the production of monoclonal antibodies (MAb) recognizing the core protein of a basement membrane-specific chondroitin sulfate proteoglycan (BM-CSPG). Using immunohistochemical techniques, we have shown that BM-CSPG is present in almost every basement membrane, one exception being the normal glomerular capillary basement membrane (GBM), where it is absent. In the present study of mature kidneys we examined the distribution of BM-CSPG in streptozocin-induced diabetes mellitus in rats. We found BM-CSPG atypically associated with the GBM of diabetic animals as early as 1 month after induction of diabetes mellitus. Immunoelectron microscopy (IEM) of affected capillary loops showed BM-CSPG present in the subendothelial matrix in areas of GBM thickening and absent in areas where the GBM appears to be of normal thickness. Moreover, the association of BM-CSPG with regions of the pericapillary GBM affects the morphology of the capillary endothelial cells within these areas, directly displacing the cell body from the GBM proper and causing loss of fenestrae. These new data on BM-CSPG distribution reflect abnormal glomerular extracellular matrix protein biosynthesis/turnover in diabetes and suggest that BM-CSPG in the GBM might in turn affect normal capillary structure and/or function.

scholarly journals Thickening of glomerular basement membrane in spontaneously diabetic rats

Diabetologia ◽  
1978 ◽  
Vol 15 (4) ◽  
pp. 309-312 ◽  
Author(s):  
S. Yagihashi ◽  
Y. Goto ◽  
M. Kakizaki ◽  
N. Kaseda
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Diabetic Rats ◽  
Spontaneously Diabetic Rats

Alterations in glomerular permeability in streptozotocin-induced diabetic rats

1996 ◽  
Vol 86 (2) ◽  
pp. 57-62 ◽  
Author(s):  
RB Boyd ◽  
VW Thompson ◽  
J Atkin
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Diabetic Rats ◽  
Capillary Wall ◽  
Diabetic Rat ◽  
Electron Microscopic ◽  
Glomerular Permeability ◽  
Glomerular Capillary Wall ◽  
Capillary Lumen ◽  
Glomerular Capillary

The alteration in glomerular basement membrane permeability associated with microangiopathy in streptozotocin-induced diabetic rats was studied by determining the movement across the glomerular basement membrane of anionic ferritin probes injected into rats at different points in the development of the disease. Visualization of the concentration gradient of anionic ferritin and changes in ultrastructure was accomplished by electron microscopic examination of renal tissue prepared from both control and diabetic rats. In all control rats, the anionic ferritin did not leave the glomerular capillary lumen, nor were there any changes in the normal morphology of the glomerular capillary wall. In the diabetic animals, the concentration of anionic ferritin shifted from the capillary lumen in the abluminal direction. Distinct morphologic changes, such as widening of endothelial intercellular junctions, focal detachment of podocyte foot processes, and extensive thickening of the glomerular basement membrane, were noted in the diabetic rat, and these changes appear to correlate with the observed increase in permselectivity of anionic ferritin across the glomerular capillary wall.

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